Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,567,255 A→T 5.9% intergenic (+59/‑262) pcaR → / → pcaK pca regulon transcriptional regulator/4‑hydroxybenzoate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,567,2550AT 5.9% 210.7 / 2.5 68intergenic (+59/‑262)pcaR/pcaKpca regulon transcriptional regulator/4‑hydroxybenzoate transporter
Reads supporting (aligned to +/- strand):  ref base A (32/32);  new base T (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GGGCGGGTGACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCGCACCC  >  NC_002947/1567107‑1567402
                                                                                                                                                    |                                                                                                                                                   
gggcgggTGACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCGGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGa                                                                                                                                                     <  1:318770/149‑1 (MQ=255)
 ggcgggTGACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGa                                                                                                                                                     >  6:54184/1‑148 (MQ=255)
     ggTGACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAAc                                                                                                                                                <  7:274650/149‑1 (MQ=255)
        gACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGca                                                                                                                                             <  3:337801/149‑1 (MQ=255)
         aCCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGca                                                                                                                                             >  6:287780/1‑148 (MQ=255)
           ccGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGTTTATCGCACAg                                                                                                                                          <  6:226332/149‑1 (MQ=255)
           ccGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAACCGCACAg                                                                                                                                          >  8:135955/1‑149 (MQ=255)
           ccGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAg                                                                                                                                          >  5:330100/1‑149 (MQ=255)
            cGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAgt                                                                                                                                         >  2:99145/1‑149 (MQ=255)
               aGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACAAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTc                                                                                                                                      <  8:223373/149‑1 (MQ=255)
                   aaCTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGc                                                                                                                                  >  3:90502/1‑149 (MQ=255)
                        gAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGt                                                                                                                             >  2:71673/1‑149 (MQ=255)
                             gcGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTAGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAAtt                                                                                                                        <  1:182709/149‑1 (MQ=255)
                                gTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCg                                                                                                                     >  5:380986/1‑149 (MQ=255)
                                     tGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCAcc                                                                                                                >  4:167222/1‑149 (MQ=255)
                                      gCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCt                                                                                                               >  3:142261/1‑149 (MQ=255)
                                          aTTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTcggc                                                                                                           <  4:97976/149‑1 (MQ=255)
                                                   gCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGa                                                                                                    <  4:142261/147‑1 (MQ=255)
                                                   gCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGAt                                                                                                   >  8:14768/1‑148 (MQ=255)
                                                     agccagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTaa                                                                                                >  8:356543/1‑149 (MQ=255)
                                                     agccagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTaa                                                                                                >  4:98867/1‑149 (MQ=255)
                                                      gccagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAAt                                                                                               >  2:343874/1‑149 (MQ=255)
                                                      gccagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAAt                                                                                               <  5:54184/149‑1 (MQ=255)
                                                      gccagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAAt                                                                                               >  8:31645/1‑149 (MQ=255)
                                                       ccagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTcgg                                                                                                            >  7:204289/1‑135 (MQ=255)
                                                       ccagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTcgg                                                                                                            <  8:204289/135‑1 (MQ=255)
                                                        cagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGt                                                                                             <  5:207493/149‑1 (MQ=255)
                                                        cagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGt                                                                                             <  4:355524/149‑1 (MQ=255)
                                                           ccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGtctc                                                                                          <  1:198688/149‑1 (MQ=255)
                                                             gggACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGATCAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGtctctc                                                                                        <  4:308590/149‑1 (MQ=255)
                                                                   tttGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACg                                                                                  >  5:311118/1‑149 (MQ=255)
                                                                       ccaccaGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCg                                                                              >  8:308407/1‑149 (MQ=255)
                                                                         accaGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGc                                                                            >  6:62334/1‑149 (MQ=255)
                                                                             gttgttTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGc                                                                        <  3:364941/149‑1 (MQ=255)
                                                                              ttgttTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGcc                                                                       >  8:322037/1‑149 (MQ=255)
                                                                                 tttGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgac                                                                    >  1:42832/1‑149 (MQ=255)
                                                                                 tttGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgac                                                                    >  3:166490/1‑149 (MQ=255)
                                                                                 tttGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccga                                                                     <  3:98867/148‑1 (MQ=255)
                                                                                  ttGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgacc                                                                   >  4:320944/1‑149 (MQ=255)
                                                                                     gTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTGGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgaccga                                                                 >  2:232336/1‑148 (MQ=255)
                                                                                     gTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgaccgtt                                                                <  5:62334/149‑3 (MQ=255)
                                                                                          gaCCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAg                                                            >  5:134139/1‑148 (MQ=255)
                                                                                           aCCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGaa                                                          <  1:343874/149‑1 (MQ=255)
                                                                                            ccGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACTGTCGGCTAGCCGACCGAACAAGaa                                                          <  7:31645/148‑1 (MQ=255)
                                                                                              gCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTGGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGaaaaa                                                       <  1:232336/149‑1 (MQ=255)
                                                                                              gCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGaaaaa                                                       <  7:308407/149‑1 (MQ=255)
                                                                                                aGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAaca                                                     <  4:119800/149‑1 (MQ=255)
                                                                                                aGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAaca                                                     <  7:135955/149‑1 (MQ=255)
                                                                                                 gggAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAacac                                                    <  7:239625/149‑1 (MQ=255)
                                                                                                  ggAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACt                                                   <  3:320944/149‑1 (MQ=255)
                                                                                                      cACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTagag                                               <  6:330100/149‑1 (MQ=255)
                                                                                                      cACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTagag                                               <  6:380986/149‑1 (MQ=255)
                                                                                                        cTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAgg                                              >  6:69139/1‑148 (MQ=255)
                                                                                                               gCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGcactcac                                       >  5:189632/1‑148 (MQ=255)
                                                                                                                        cAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGTGTGAATTGCGTCACCTCGGCCTGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATa                              <  1:71673/148‑1 (MQ=255)
                                                                                                                         aGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACt                            <  2:42832/149‑1 (MQ=255)
                                                                                                                          gAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACt                            >  3:272929/1‑148 (MQ=255)
                                                                                                                          gAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACt                            <  4:272929/148‑1 (MQ=255)
                                                                                                                          gAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTg                           >  7:102657/1‑149 (MQ=255)
                                                                                                                              aGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCAc                       >  1:352632/1‑149 (MQ=255)
                                                                                                                               ggCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCAcc                      <  7:322037/149‑1 (MQ=255)
                                                                                                                                  aaaaCCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCg                   >  1:392407/1‑149 (MQ=255)
                                                                                                                                     aCCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCgctg                <  5:69139/149‑1 (MQ=255)
                                                                                                                                      cacgacacTGTGCGTTTATCGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCgctgc               >  2:148152/8‑149 (MQ=255)
                                                                                                                                            acTGTGCGTTTATCGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTa                                                  <  5:327912/107‑1 (MQ=255)
                                                                                                                                            acTGTGCGTTTATCGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTa                                                  >  6:327912/2‑108 (MQ=255)
                                                                                                                                             cTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGcc        <  4:90502/149‑1 (MQ=255)
                                                                                                                                             cTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGc         <  2:392407/148‑1 (MQ=255)
                                                                                                                                                   gATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACGTCCGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCCCACAAAAAAAACACAAGAGGCACTCACTCATGAATACTCTCACCTCGCTGCTGCGGCCGCcccc  >  6:77878/1‑149 (MQ=255)
                                                                                                                                                   gATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCGCAccc  >  3:386630/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGGCGGGTGACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGTGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCGCACCC  >  NC_002947/1567107‑1567402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑