Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,594,061 T→C 6.5% intergenic (‑259/+42) PP_1399 ← / ← kgtP hypothetical protein/alpha‑ketoglutarate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,594,0610TC 6.5% 215.3 / 3.8 77intergenic (‑259/+42)PP_1399/kgtPhypothetical protein/alpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base T (30/41);  new base C (2/3);  total (33/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCT  >  NC_002947/1593913‑1594206
                                                                                                                                                    |                                                                                                                                                  
tGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCt                                                                                                                                                    >  8:226770/1‑149 (MQ=255)
   aTTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGTAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCa                                                                                                                                                 >  8:309037/1‑149 (MQ=255)
    tttGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCAt                                                                                                                                                <  5:84118/149‑1 (MQ=255)
    tttGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCAt                                                                                                                                                >  6:102669/1‑149 (MQ=255)
        ggCGCGTATGTCGTGCTTCAGGCCGCAAGACTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCaa                                                                                                                                            <  2:369193/149‑1 (MQ=255)
              tATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGgc                                                                                                                                      <  4:351585/149‑1 (MQ=255)
               aTGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGgcg                                                                                                                                     >  4:294648/1‑149 (MQ=255)
               aTGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTAGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAATGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGgcg                                                                                                                                     >  8:152674/1‑149 (MQ=255)
                  tCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGcc                                                                                                                                   <  3:142495/148‑1 (MQ=255)
                    gTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCt                                                                                                                                >  3:314472/1‑149 (MQ=255)
                     tGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCtt                                                                                                                               <  1:56268/149‑1 (MQ=255)
                       cTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGCCCCttt                                                                                                                              <  5:102669/148‑1 (MQ=255)
                        ttCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                              >  5:99112/1‑147 (MQ=255)
                        ttCAGGCCGCAAGGCTGGCGTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGGCTGAATAGAGACGGGGCGCTCCATGCAATGGGGCGCCCCTTTTa                                                                                                                            <  5:133228/149‑1 (MQ=255)
                           aGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTa                                                                                                                         >  7:26986/1‑149 (MQ=255)
                            ggCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTAc                                                                                                                        <  8:136300/149‑1 (MQ=255)
                              ccGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGTAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGCCCCttttctattca                                                                                                                      <  7:309037/149‑8 (MQ=38)
                              ccGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGATTAGAAAAGGGGCGCCCCATTGCATGGAGCGCCCCttttctattca                                                                                                                      <  1:275706/149‑8 (MQ=38)
                               cGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgc                                                                                                                     >  2:81330/1‑149 (MQ=255)
                                gCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgcc                                                                                                                    <  3:294648/149‑1 (MQ=255)
                                gCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgc                                                                                                                     >  6:320841/1‑148 (MQ=255)
                                 cAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgcca                                                                                                                   <  8:318003/149‑1 (MQ=255)
                                  aaGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGTGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                              >  2:148895/1‑137 (MQ=255)
                                  aaGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGTGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                              <  1:148895/137‑1 (MQ=255)
                                         gctTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCg                                                                                                           >  8:18115/1‑149 (MQ=255)
                                               gAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACt                                                                                                      >  2:55521/1‑148 (MQ=255)
                                                  cGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGt                                                                                                  <  2:362755/149‑1 (MQ=255)
                                                   gTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTg                                                                                                 <  7:226770/149‑1 (MQ=255)
                                                       gcgcgGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCt                                                                                                                                <  3:250149/114‑1 (MQ=255)
                                                       gcgcgGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCt                                                                                                                                >  4:250149/1‑114 (MQ=255)
                                                         gcgGGCGCCAACGTTCTGCCATCCATTGCAGCTTGATAATGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATc                                                                                           <  5:97322/149‑1 (MQ=255)
                                                            ggCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCcgc                                                                                        >  8:324179/1‑149 (MQ=255)
                                                                           ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGTAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCGAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCttt                                                                            <  4:176752/146‑2 (MQ=255)
                                                                           ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTgg                                                                           <  1:55521/147‑1 (MQ=255)
                                                                           ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGgtg                                                                         >  1:156388/1‑149 (MQ=255)
                                                                           ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGgtg                                                                         >  8:236378/1‑149 (MQ=255)
                                                                           ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGgtg                                                                         >  1:163612/1‑149 (MQ=255)
                                                                           ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGgt                                                                          <  4:326511/148‑1 (MQ=255)
                                                                              gccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCt                                                                      <  7:236378/149‑1 (MQ=255)
                                                                                 aTTGCAGCTTGATAAGGTGGAATTAATAGGTCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑Tc                                                                  <  6:2355/149‑1 (MQ=255)
                                                                                 aTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑Tc                                                                  <  1:154737/149‑1 (MQ=255)
                                                                                 aTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑Tc                                                                  <  6:7450/149‑1 (MQ=255)
                                                                                 aTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑Tc                                                                  <  5:320841/149‑1 (MQ=255)
                                                                                     cAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATg                                                               <  1:22687/148‑1 (MQ=255)
                                                                                      agattGCTAAGGTGGAATTAATCTGGCTTTAAGAAGGAAAAGGCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCGTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATgg                                                              <  4:248963/145‑1 (MQ=255)
                                                                                      aGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGt                                                             <  7:18115/149‑1 (MQ=255)
                                                                                           gATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTcgc                                                          <  6:99112/147‑1 (MQ=255)
                                                                                             tAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTaa                                                      <  5:138664/149‑1 (MQ=255)
                                                                                             tAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTa                                                       >  6:200535/1‑148 (MQ=255)
                                                                                                ggTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAAc                                                    >  3:155209/1‑148 (MQ=255)
                                                                                                 gTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtgct                                                                                 <  7:201456/119‑1 (MQ=255)
                                                                                                 gTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCGCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtgct                                                                                 >  8:201456/1‑119 (MQ=255)
                                                                                                       ttAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCagcagc                                             >  7:104639/1‑148 (MQ=255)
                                                                                                              ggCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGc                                     >  4:30744/1‑149 (MQ=255)
                                                                                                                cGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGa                                   <  7:324179/149‑1 (MQ=255)
                                                                                                                  ttAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATCGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTCGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATa                                 <  8:285839/149‑1 (MQ=255)
                                                                                                                    aagaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATa                                 >  4:144593/1‑147 (MQ=255)
                                                                                                                      gaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAgg                             <  3:30744/149‑1 (MQ=255)
                                                                                                                      gaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAgg                             >  7:345803/1‑149 (MQ=255)
                                                                                                                      gaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAgg                             <  5:386256/149‑1 (MQ=255)
                                                                                                                         ggAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCATCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCgg                          >  3:198112/1‑149 (MQ=255)
                                                                                                                         ggAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCgg                          <  8:26986/149‑1 (MQ=255)
                                                                                                                         ggAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCgg                          >  8:115369/1‑149 (MQ=255)
                                                                                                                         ggAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCgg                          >  2:80872/1‑149 (MQ=255)
                                                                                                                            aaaGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGa                       <  7:115369/149‑1 (MQ=255)
                                                                                                                             aattCTGAATACTAAACGGGCGCTCCATGCAATGGGGCGCCCCGTTTATTACTGCCAGGCCATCGGTACTCACTTGTAATCCGCGATTGCTGCTTGGTGTCTTCTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGa                       <  1:80872/146‑1 (MQ=255)
                                                                                                                             aaGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGAc                      <  5:200535/149‑1 (MQ=255)
                                                                                                                              aGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACg                     <  3:144593/149‑1 (MQ=255)
                                                                                                                                   gAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc                  >  3:171670/1‑147 (MQ=255)
                                                                                                                                   gAATAGAAAAGGCGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTTCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAcaa                >  4:153226/1‑147 (MQ=255)
                                                                                                                                    aaTAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCa                <  4:171670/148‑1 (MQ=255)
                                                                                                                                        gAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAg            >  3:207805/1‑148 (MQ=255)
                                                                                                                                        gAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAg            >  2:305428/1‑148 (MQ=255)
                                                                                                                                         aaaaGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGaa          <  4:314472/149‑1 (MQ=255)
                                                                                                                                                gcgcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGctc   >  2:72555/1‑149 (MQ=255)
                                                                                                                                                 cgcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCATCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGctct  <  1:81330/149‑1 (MQ=255)
                                                                                                                                                 cgcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGctc   >  1:110538/1‑148 (MQ=255)
                                                                                                                                                 cgcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGctc   <  2:110538/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTT‑TCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCT  >  NC_002947/1593913‑1594206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑