Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,596,814 T→A 5.0% E66V (GAA→GTA) ‡ dctD‑III ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,596,8140TA 5.0% 254.0 / 2.2 79E66V (GAA→GTA) ‡dctD‑IIIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (34/41);  new base A (2/2);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

CGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCTCAGCCATTGCTCGA  >  NC_002947/1596667‑1596958
                                                                                                                                                    |                                                                                                                                                
cgcAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTc                                                                                                                                                 >  6:104436/1‑149 (MQ=255)
   aGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAg                                                                                                                                              <  8:160450/149‑1 (MQ=255)
      cTGCACAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCaa                                                                                                                                           <  4:373393/149‑1 (MQ=255)
         cccAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAAt                                                                                                                                          <  5:104436/147‑1 (MQ=255)
         cccAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTg                                                                                                                                        >  4:80492/1‑149 (MQ=255)
          ccAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATTGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGGAGGCGTTCGAGCAATTGc                                                                                                                                       <  6:200012/149‑1 (MQ=255)
          ccAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGc                                                                                                                                       <  3:349689/149‑1 (MQ=255)
           cAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGACGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTg                                                                                                                                        >  8:366431/1‑147 (MQ=255)
            aGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAg                                                                                                                                     <  3:204159/149‑1 (MQ=255)
             gCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAgg                                                                                                                                    <  3:196513/149‑1 (MQ=255)
                 gTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGccat                                                                                                                                <  5:221778/149‑1 (MQ=255)
                  tCTTGCGGCGTGAAGTGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCCGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGccatc                                                                                                                               <  1:311005/149‑1 (MQ=255)
                  tCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGACCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGccatc                                                                                                                               >  7:371687/1‑149 (MQ=255)
                    ttGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGccatcca                                                                                                                             >  3:283647/1‑149 (MQ=255)
                       cggcgTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGc                                                                                                                          <  6:329647/149‑1 (MQ=255)
                       cggcgTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGA‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGc                                                                                                                          >  4:111644/1‑149 (MQ=255)
                          cgTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCg                                                                                                                       <  3:111644/149‑1 (MQ=255)
                             gAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCAATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATAACGGGCAGTTCGGGGACGTACGCCTGCAGGCGTTCGACCAATTGCAGGCCTACCATGCCCgg                                                                                                                      >  1:267270/1‑148 (MQ=38)
                              aaGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTc                                                                                                                                                 <  8:187432/119‑1 (MQ=255)
                              aaGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGAAGGTCGGGGTCGTTCGCCTGCAGGCGTTc                                                                                                                                                 >  7:187432/1‑119 (MQ=255)
                               aGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATg                                                                                                                  >  5:376331/1‑149 (MQ=255)
                               aGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATg                                                                                                                  >  3:323275/1‑149 (MQ=255)
                                gggTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTACAGGCCATCCATGCCCGGCATgc                                                                                                                 <  2:186898/149‑1 (MQ=255)
                                 ggTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATgcg                                                                                                                >  8:231730/1‑149 (MQ=255)
                                  gTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATgcg                                                                                                                >  3:298690/1‑148 (MQ=255)
                                         cAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGc                                                                                                        >  6:288205/1‑149 (MQ=255)
                                            gAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCtga                                                                                                     <  6:298951/149‑1 (MQ=255)
                                            gAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCtga                                                                                                     >  4:167673/1‑149 (MQ=255)
                                              aGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCtgatg                                                                                                   <  4:283647/149‑1 (MQ=255)
                                                  aTAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCa                                                                                               <  7:7224/149‑1 (MQ=255)
                                                    aGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCCTCCATGCCCGGCATGCGCACATCGCTGATGATCAcc                                                                                             >  3:299168/1‑149 (MQ=255)
                                                    aGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCAcc                                                                                             >  2:14288/1‑149 (MQ=255)
                                                      gCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCAccc                                                                                            >  3:110502/1‑148 (MQ=255)
                                                       ccccGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCa                                                                                          <  2:267270/149‑1 (MQ=255)
                                                        cccGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAg                                                                                         >  7:47596/1‑149 (MQ=255)
                                                        cccGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAg                                                                                         >  7:185558/1‑149 (MQ=255)
                                                        cccGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAg                                                                                         >  7:106452/1‑149 (MQ=255)
                                                         ccGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAgg                                                                                        <  3:252305/149‑1 (MQ=255)
                                                                 c‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGtgct                                                                                >  6:41054/1‑149 (MQ=255)
                                                                   aTGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGACATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGtgctg                                                                               >  6:311219/1‑149 (MQ=255)
                                                                    tGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGtgctgc                                                                              <  5:288205/149‑1 (MQ=255)
                                                                     ggCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCAt                                                                                                                   <  1:343042/111‑1 (MQ=255)
                                                                     ggCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCAt                                                                                                                   >  2:343042/1‑111 (MQ=255)
                                                                             ccGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGgtgt                                                                     <  1:232257/149‑1 (MQ=255)
                                                                              cGCCATCGGTACATCCCCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGtgtg                                                                    <  6:248964/149‑1 (MQ=255)
                                                                                 cATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCa                                                                 <  3:80492/149‑1 (MQ=255)
                                                                                   tCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAgg                                                               <  4:110502/149‑1 (MQ=255)
                                                                                   tCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAgg                                                               >  6:274991/1‑149 (MQ=255)
                                                                                          aTCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCtt                                                        >  3:65105/1‑149 (MQ=255)
                                                                                           tCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTc                                                       >  1:233318/1‑149 (MQ=255)
                                                                                                gTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCTCGGCATGCGCACATCGCTGATGATCACCCCTGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGgcgc                                                  <  5:224160/149‑1 (MQ=255)
                                                                                                 tGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGcc                                                                  <  1:145367/132‑1 (MQ=255)
                                                                                                 tGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGcc                                                                  >  2:145367/1‑132 (MQ=255)
                                                                                                   aCCGGGCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCgcga                                                <  2:337942/148‑1 (MQ=255)
                                                                                                    ccGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCgcgagc                                              <  8:47596/149‑1 (MQ=255)
                                                                                                     cGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGACATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCgcgagcg                                             <  5:311219/149‑1 (MQ=255)
                                                                                                       gTCAGCAGAATTACGGGCAGGCCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGaa                                           <  7:366431/149‑1 (MQ=255)
                                                                                                        tCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGaac                                          <  8:185558/149‑1 (MQ=255)
                                                                                                          agcagAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGaacaa                                        <  6:376331/149‑1 (MQ=255)
                                                                                                             agAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTc                                     <  4:298690/149‑1 (MQ=255)
                                                                                                             agAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTc                                     >  4:223282/1‑149 (MQ=255)
                                                                                                                      ggCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGaa                            <  8:371687/149‑1 (MQ=255)
                                                                                                                      ggCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCCGaa                            <  5:351732/149‑1 (MQ=255)
                                                                                                                       gCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCgcgagcg                                             >  2:21585/1‑131 (MQ=255)
                                                                                                                       gCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCgcgagcg                                             <  1:21585/131‑1 (MQ=255)
                                                                                                                       gCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAg                           >  2:123864/1‑149 (MQ=255)
                                                                                                                         aGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGc                          >  4:258266/1‑148 (MQ=255)
                                                                                                                           gTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGGGCGAGCGAACAACTCCACGCTGAAGc                          >  6:101087/1‑146 (MQ=255)
                                                                                                                              ggggTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGGGCGAGCGAACAACTCCACGCTGAAGCCAGAc                     <  5:101087/148‑1 (MQ=255)
                                                                                                                              ggggTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGAc                     <  3:167673/148‑1 (MQ=255)
                                                                                                                               gggTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAg                   <  1:14288/149‑1 (MQ=255)
                                                                                                                                  tcgaacGCCTGCAGGCGAACGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCt                >  1:193284/6‑149 (MQ=255)
                                                                                                                                  tcgaacGCCTGCAGGCGAACGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCt                >  1:187235/6‑149 (MQ=255)
                                                                                                                                          cTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCTCAGCCAtt        >  6:290127/1‑149 (MQ=255)
                                                                                                                                          cTGCAGGCGAACGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCTCAGCCAtt        <  2:193284/149‑1 (MQ=255)
                                                                                                                                          cTGCAGGCGAACGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCTCAGCCAtt        <  2:187235/149‑1 (MQ=255)
                                                                                                                                             cAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCTCAGCCATTg       <  5:274991/147‑1 (MQ=255)
                                                                                                                                             cAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCTCAGCCATTGCt     <  8:106452/149‑1 (MQ=255)
                                                                                                                                                  gTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCTCAGCCATTGCTCGa  >  3:48729/1‑147 (MQ=255)
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CGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGC‑ATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCTGCAGGCGTTCGAGCAATTGCAGGCCATCCATGCCCGGCATGCGCACATCGCTGATGATCACCCCAGGGAAGTGCTGCGGCAGGTGTGCCAGGCACGCTTCGGCGCGAGCGAACAACTCCACGCTGAAGCCAGACAGGCTCAGCCATTGCTCGA  >  NC_002947/1596667‑1596958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑