Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,625,208 A→C 19.7% intergenic (+31/+26) PP_1425 → / ← nadB hypothetical protein/L‑aspartate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,625,2080AC19.7% 84.1 / 18.0 50intergenic (+31/+26)PP_1425/nadBhypothetical protein/L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base A (24/15);  new base C (5/5);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

GCTGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCA  >  NC_002947/1625063‑1625352
                                                                                                                                                 |                                                                                                                                                
gCTGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGcc                                                                                                                                               >  2:225966/1‑149 (MQ=255)
      ggAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCTCGCCCGCGaa                                                                                                                                         <  3:326430/149‑1 (MQ=255)
         aaGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCTCGGGCTCGCCCGCGAAgaa                                                                                                                                      <  8:75875/149‑3 (MQ=255)
            ccAGTGTATGCCTCAGGATGCGCTCGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                         <  3:294253/143‑1 (MQ=255)
             ctgtgtATTCCTCAGGATGCGCTAGGGGCTGACGAGCAGCGGCGCTTGCGGGTGCGGTTTAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGCCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCacg                                                                                                                                  <  2:266566/147‑1 (MQ=255)
             cAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGAGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCac                                                                                                                                   >  2:11643/1‑148 (MQ=255)
                       cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGatat                                                                                                                        >  7:120921/1‑149 (MQ=255)
                       cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGata                                                                                                                         >  2:395522/1‑148 (MQ=255)
                        tCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGatat                                                                                                                        >  4:367773/1‑148 (MQ=255)
                        tCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGatat                                                                                                                        >  7:5046/1‑148 (MQ=255)
                         ccggATGCGCTAGGGGCGGACGAGCATCGGCGCTTGCGGGTGCGGTTGACGGTCCGCCGGCGGCGGTGGGCTGCGGGGAGCCGGGCATAGAGCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCa                                                                                                                      <  7:120683/147‑1 (MQ=255)
                            gATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACCCCGATATCagac                                                                                                                   >  5:68045/1‑148 (MQ=255)
                                cgcTGGGGGCTGACGAGCATCGGCGCTTGGGGGTGCGGTTGAAGTTCCGCGGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACGGGGTTAGCTTCTGCGCGGGCGCGCCCGCGAAGGCCACGACGCCGATATCAGAGCggg                                                                                                               <  3:367773/149‑1 (MQ=255)
                                cgcTCGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTTAATTTCAGGCGGCGGCGCTGGGCTGGTGTGAGCGGGGCATATATCGAGACCGGGTTAGCTTCTTCGCGGGCTAGCCCGGGAAGGCCACGACGCCGATATCAGAGCgg                                                                                                                <  6:195514/148‑1 (MQ=255)
                                        gCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGAt                                                                                                       <  8:5046/149‑1 (MQ=255)
                                          tGACGAGCATCGGAGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATgg                                                                                                     >  3:8856/1‑149 (MQ=255)
                                            aCGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGgtg                                                                                                   >  4:285716/1‑149 (MQ=255)
                                              gAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTc                                                                                                 >  7:82288/1‑149 (MQ=255)
                                                    cggcggTTGCGGGTGGGGTTGAAGTTCCGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGCGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTg                                                                                             <  7:356416/147‑1 (MQ=255)
                                                       cgtttGCGGGGGCGGTTGAAGTTCAGCCGGGGGCGGGGGGCTGCGGTGAGCCGGGAATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTc                                                                                        <  4:370314/146‑1 (MQ=255)
                                                             cGGGTGCGGTTGAAGTTCAGCGGGCGGGGGGGGGCGGGGGTGAGCCGGGAATATATCGACCCCGGGTTAGCTTCTTCGGGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCa                                                                                   <  8:120921/148‑1 (MQ=255)
                                                              gggTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGCCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAg                                                                                  >  8:155071/1‑148 (MQ=255)
                                                                        gAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGa                                                                        <  1:225966/148‑1 (MQ=255)
                                                                           gTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCa                                                                                                                                    >  3:92504/1‑85 (MQ=255)
                                                                           gTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCa                                                                                                                                    <  4:92504/85‑1 (MQ=255)
                                                                                gcgggcggcGGGGGTCTGGGGTGCGCCGGTCTTATTTCGACACGGGTTTAGTTTCTTGGGGGGCTCGCCGGGGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                               <  1:395522/146‑1 (MQ=255)
                                                                                 ccGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgtg                                                              >  1:346199/1‑149 (MQ=255)
                                                                                                       aGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTc                                        >  5:371477/1‑149 (MQ=255)
                                                                                                          cGGGCATAGATCGAGCCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCtt                                     <  3:285716/149‑1 (MQ=255)
                                                                                                            ggCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgc                                   >  3:7042/1‑149 (MQ=255)
                                                                                                             gCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgc                                   >  4:97800/1‑148 (MQ=255)
                                                                                                                 aGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTg                               >  6:134987/1‑148 (MQ=255)
                                                                                                                   aTCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCAt                            >  6:377617/1‑149 (MQ=255)
                                                                                                                   aTCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCAt                            >  2:7872/1‑149 (MQ=255)
                                                                                                                      gagaCCGGGTTAGCTTCTTCGCGGGCGCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGc                         >  1:392775/1‑149 (MQ=255)
                                                                                                                      gagaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATgg                          <  5:377617/148‑1 (MQ=255)
                                                                                                                       agaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCt                        <  6:371477/149‑1 (MQ=255)
                                                                                                                          ccGGCTTAGCTTCTGGGCGGGCGTGCCCGCGAAGGCGACGTCGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGTCCTCGTCCAGCATCCCTGGATAATCCTGTGTGTAATCGAACGCTCGGCTTTCGTTGCGCTGCATGGCTGAc                     <  6:68045/149‑1 (MQ=255)
                                                                                                                           cGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGa                      >  1:89760/1‑147 (MQ=255)
                                                                                                                               ttAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGat                >  8:90592/1‑149 (MQ=255)
                                                                                                                                 aGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatca              >  1:41167/1‑149 (MQ=255)
                                                                                                                                    ttcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGCCAGGatcat             >  4:111846/1‑147 (MQ=255)
                                                                                                                                     ccttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAg          >  1:345394/2‑149 (MQ=255)
                                                                                                                                      cttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGAtcatca           <  1:7872/147‑1 (MQ=255)
                                                                                                                                       ttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATgg                          >  6:373717/1‑131 (MQ=255)
                                                                                                                                       ttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATgg                          <  5:373717/131‑1 (MQ=255)
                                                                                                                                       ttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCt        >  4:134926/1‑149 (MQ=255)
                                                                                                                                       ttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCt        >  2:315359/1‑149 (MQ=255)
                                                                                                                                           cgGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGc    >  2:38534/1‑149 (MQ=255)
                                                                                                                                             ggCGAGGCCGGGCAGGCCACGACGCCGATATCAGAGCGGGGTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTTGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCa  <  8:82288/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCTGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCA  >  NC_002947/1625063‑1625352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑