Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,639,565 A→G 7.3% intergenic (‑155/‑156) czcR‑III ← / → PP_1439 response regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,639,5650AG 7.3% 153.7 / 2.7 55intergenic (‑155/‑156)czcR‑III/PP_1439response regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base G (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

CTCGGTATGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGG  >  NC_002947/1639417‑1639712
                                                                                                                                                    |                                                                                                                                                   
cTCGGTATGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGa                                                                                                                                                     <  6:78868/149‑1 (MQ=255)
    gTATGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTa                                                                                                                                                  >  8:79232/1‑148 (MQ=255)
         ttGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCcg                                                                                                                                            >  7:9795/1‑149 (MQ=255)
                 ggACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCGTg                                                                                                                                      >  6:376216/1‑147 (MQ=255)
                 ggACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTg                                                                                                                                      >  5:253532/1‑147 (MQ=255)
                 ggACGATTCTTGACCCTGTAGTGACATCAGAGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCATGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTTTCTTTTCGGGCCTCTTCGCGAGTTAACCCGCGCCTg                                                                                                                                      >  8:59874/1‑147 (MQ=255)
                         cTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACgg                                                                                                                             <  8:122719/148‑1 (MQ=255)
                             aCCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGttt                                                                                                                         <  6:253532/148‑1 (MQ=255)
                             aCCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTa                                                                                                                        <  5:330605/149‑1 (MQ=255)
                              cccTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAc                                                                                                                       <  8:144000/149‑1 (MQ=255)
                               ccTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc                                                                                                                      <  4:316576/149‑1 (MQ=255)
                                   tAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCcgc                                                                                                                   <  4:223080/148‑1 (MQ=255)
                                     gTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGaa                                                                                                                <  3:335525/149‑1 (MQ=25)
                                     gTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGa                                                                                                                 <  6:246591/148‑1 (MQ=255)
                                      tGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCcgcg                                                                                                                  >  7:5335/1‑146 (MQ=255)
                                        aCATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAagag                                                                                                             <  8:333641/149‑1 (MQ=255)
                                          aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTcgc                                                                                                                   >  2:346908/1‑141 (MQ=25)
                                          aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTcgc                                                                                                                   <  1:346908/141‑1 (MQ=25)
                                          aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                           >  7:59634/1‑149 (MQ=255)
                                           tCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAGGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGcc                                                                                                          >  7:370100/1‑149 (MQ=255)
                                             aGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGccc                                                                                                         >  7:347253/1‑148 (MQ=255)
                                             aGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGccc                                                                                                         <  4:104718/148‑1 (MQ=255)
                                                    aGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCCAACAGTc                                                                                                 >  8:148645/1‑149 (MQ=255)
                                                     gcgcCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTcc                                                                                                <  7:79232/149‑1 (MQ=255)
                                                      cgcCCAAATGTTCCGCCATGAAAAACGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGCCCTCTTCGCGAGTAAACCCGCGCCTGTAGGACCGGTTTTACCCGCTAAGAGGCCCGAACAGTCca                                                                                               <  8:370100/149‑1 (MQ=255)
                                                        cccaAATGTTACGGCATGAAAAAAGCCCTGCGGTTCATATACCGCATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCTAGTAAACCCGCGCCTGTAGTAACGGGTTTACCCGCTAAGTGGCCCGAACAGTCcaca                                                                                             <  8:347253/149‑1 (MQ=255)
                                                                 tACGGCATGAAAAAAGCCCGGCGGTGCAGAAAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCTAAGAGGCCCGAACAGTCCACACAAGGCca                                                                                     <  4:113923/148‑1 (MQ=255)
                                                                     gCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTg                                                                                >  6:86297/1‑149 (MQ=255)
                                                                       aTGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCa                                                                              <  5:354117/149‑1 (MQ=255)
                                                                       aTGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGACTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGGTTACCCGCGAAGAGGCCCGATCAGTGCACACAAGGCCACATTg                                                                                >  8:200474/1‑147 (MQ=255)
                                                                                    tGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCca                                                                                     >  3:164561/1‑129 (MQ=255)
                                                                                      cGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCg                                                               >  7:215146/1‑149 (MQ=255)
                                                                                           cataTAACGGATTTTTAATTCTGCTGTAATCTGACTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTAACCCGCGAAGAGTCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCa                                                          <  7:200474/146‑1 (MQ=38)
                                                                                                 aCGGATTTGTAATTCTGCTGTTTTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAATAGTCCACACAAGGCCACATTGCAGGACTGTAATCCTTGCCTCACCTTg                                                     <  8:59634/148‑1 (MQ=255)
                                                                                                   ggATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACgg                                                                                                                             >  4:247167/1‑74 (MQ=255)
                                                                                                   ggATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACgg                                                                                                                             <  3:247167/74‑1 (MQ=255)
                                                                                                     aTTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGt                                                 >  5:161120/1‑148 (MQ=255)
                                                                                                         gTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTagc                                             >  6:279718/1‑148 (MQ=255)
                                                                                                          tAATCCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGATAAAACCCGCGCCTGTAGCAACGGGTTTACCCGCGAAGAGGTCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTagcca                                           <  8:9795/149‑1 (MQ=255)
                                                                                                            aTTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTagccagc                                         >  4:280005/1‑149 (MQ=255)
                                                                                                                tgctgTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTc                                     >  1:79463/1‑149 (MQ=255)
                                                                                                                       aTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAgg                               <  5:86297/148‑1 (MQ=255)
                                                                                                                             ctgtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCGGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGGCACATTGCAGGACTGTAATCCCCGGCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCg                        >  1:167274/1‑149 (MQ=255)
                                                                                                                              tgtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgt                       <  1:340559/149‑1 (MQ=255)
                                                                                                                              tgtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgt                       <  8:5335/149‑1 (MQ=255)
                                                                                                                              tgtctgtTCGGGCCTCTTCGCGACTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgt                       >  3:120470/1‑149 (MQ=255)
                                                                                                                                tctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGCTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                     <  8:215146/149‑1 (MQ=255)
                                                                                                                                  tgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGGCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCt                   >  5:140272/1‑149 (MQ=255)
                                                                                                                                  tgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCt                   <  6:161120/149‑1 (MQ=255)
                                                                                                                                       gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATc               <  2:79463/148‑1 (MQ=255)
                                                                                                                                       gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCg              <  5:376216/149‑1 (MQ=255)
                                                                                                                                         gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATgg                           >  4:34748/1‑134 (MQ=255)
                                                                                                                                         gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATgg                           <  3:34748/134‑1 (MQ=255)
                                                                                                                                           ctctTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGtta          >  8:182757/1‑149 (MQ=25)
                                                                                                                                            tctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGcc                                                                                      <  4:204171/72‑1 (MQ=255)
                                                                                                                                            tctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGcc                                                                                      >  3:204171/1‑72 (MQ=255)
                                                                                                                                                    aGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAgg  >  2:195271/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTCGGTATGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGG  >  NC_002947/1639417‑1639712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑