Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,639,581 T→C 8.6% intergenic (‑171/‑140) czcR‑III ← / → PP_1439 response regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,639,5810TC 8.6% 149.2 / 5.3 58intergenic (‑171/‑140)czcR‑III/PP_1439response regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/32);  new base C (3/2);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.07e-01

CTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAAC  >  NC_002947/1639442‑1639726
                                                                                                                                           |                                                                                                                                                 
cTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACgg                                                                                                                                           <  8:122719/148‑1 (MQ=255)
    aCCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGttt                                                                                                                                       <  6:253532/148‑1 (MQ=255)
    aCCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTa                                                                                                                                      <  5:330605/149‑1 (MQ=255)
     cccTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAc                                                                                                                                     <  8:144000/149‑1 (MQ=255)
      ccTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAcc                                                                                                                                    <  4:316576/149‑1 (MQ=255)
          tAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCcgc                                                                                                                                 <  4:223080/148‑1 (MQ=255)
            gTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGaa                                                                                                                              <  3:335525/149‑1 (MQ=25)
            gTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGa                                                                                                                               <  6:246591/148‑1 (MQ=255)
             tGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCcgcg                                                                                                                                >  7:5335/1‑146 (MQ=255)
               aCATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAagag                                                                                                                           <  8:333641/149‑1 (MQ=255)
                 aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTcgc                                                                                                                                 >  2:346908/1‑141 (MQ=25)
                 aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTcgc                                                                                                                                 <  1:346908/141‑1 (MQ=25)
                 aTCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                                         >  7:59634/1‑149 (MQ=255)
                  tCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAGGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGcc                                                                                                                        >  7:370100/1‑149 (MQ=255)
                    aGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGccc                                                                                                                       <  4:104718/148‑1 (MQ=255)
                    aGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGccc                                                                                                                       >  7:347253/1‑148 (MQ=255)
                           aGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCCAACAGTc                                                                                                               >  8:148645/1‑149 (MQ=255)
                            gcgcCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTcc                                                                                                              <  7:79232/149‑1 (MQ=255)
                             cgcCCAAATGTTCCGCCATGAAAAACGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGCCCTCTTCGCGAGTAAACCCGCGCCTGTAGGACCGGTTTTACCCGCTAAGAGGCCCGAACAGTCca                                                                                                             <  8:370100/149‑1 (MQ=255)
                               cccaAATGTTACGGCATGAAAAAAGCCCTGCGGTTCATATACCGCATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCTAGTAAACCCGCGCCTGTAGTAACGGGTTTACCCGCTAAGTGGCCCGAACAGTCcaca                                                                                                           <  8:347253/149‑1 (MQ=255)
                                        tACGGCATGAAAAAAGCCCGGCGGTGCAGAAAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCTAAGAGGCCCGAACAGTCCACACAAGGCca                                                                                                   <  4:113923/148‑1 (MQ=255)
                                            gCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTg                                                                                              >  6:86297/1‑149 (MQ=255)
                                              aTGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCa                                                                                            <  5:354117/149‑1 (MQ=255)
                                              aTGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGACTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGGTTACCCGCGAAGAGGCCCGATCAGTGCACACAAGGCCACATTg                                                                                              >  8:200474/1‑147 (MQ=255)
                                                           tGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCca                                                                                                   >  3:164561/1‑129 (MQ=255)
                                                             cGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCg                                                                             >  7:215146/1‑149 (MQ=255)
                                                                  cataTAACGGATTTTTAATTCTGCTGTAATCTGACTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTAACCCGCGAAGAGTCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCa                                                                        <  7:200474/146‑1 (MQ=38)
                                                                        aCGGATTTGTAATTCTGCTGTTTTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAATAGTCCACACAAGGCCACATTGCAGGACTGTAATCCTTGCCTCACCTTg                                                                   <  8:59634/148‑1 (MQ=255)
                                                                          ggATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACgg                                                                                                                                           >  4:247167/1‑74 (MQ=255)
                                                                          ggATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACgg                                                                                                                                           <  3:247167/74‑1 (MQ=255)
                                                                            aTTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGt                                                               >  5:161120/1‑148 (MQ=255)
                                                                                gTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTagc                                                           >  6:279718/1‑148 (MQ=255)
                                                                                 tAATCCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGATAAAACCCGCGCCTGTAGCAACGGGTTTACCCGCGAAGAGGTCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTagcca                                                         <  8:9795/149‑1 (MQ=255)
                                                                                   aTTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTagccagc                                                       >  4:280005/1‑149 (MQ=255)
                                                                                       tgctgTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTc                                                   >  1:79463/1‑149 (MQ=255)
                                                                                              aTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAgg                                             <  5:86297/148‑1 (MQ=255)
                                                                                                    ctgtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCGGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGGCACATTGCAGGACTGTAATCCCCGGCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCg                                      >  1:167274/1‑149 (MQ=255)
                                                                                                     tgtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgt                                     <  1:340559/149‑1 (MQ=255)
                                                                                                     tgtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgt                                     <  8:5335/149‑1 (MQ=255)
                                                                                                     tgtctgtTCGGGCCTCTTCGCGACTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgt                                     >  3:120470/1‑149 (MQ=255)
                                                                                                       tctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGCTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                                   <  8:215146/149‑1 (MQ=255)
                                                                                                         tgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGGCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCt                                 >  5:140272/1‑149 (MQ=255)
                                                                                                         tgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCt                                 <  6:161120/149‑1 (MQ=255)
                                                                                                              gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATc                             <  2:79463/148‑1 (MQ=255)
                                                                                                              gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCg                            <  5:376216/149‑1 (MQ=255)
                                                                                                                gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATgg                                         >  4:34748/1‑134 (MQ=255)
                                                                                                                gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATgg                                         <  3:34748/134‑1 (MQ=255)
                                                                                                                  ctctTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGtta                        >  8:182757/1‑149 (MQ=25)
                                                                                                                   tctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGcc                                                                                                    <  4:204171/72‑1 (MQ=255)
                                                                                                                   tctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGcc                                                                                                    >  3:204171/1‑72 (MQ=255)
                                                                                                                           aGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAgg                >  2:195271/1‑148 (MQ=255)
                                                                                                                            gTAAACCCGTTCCTACAGGCGCGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACATTGCCGTTAGCCAGCCCTCGCTAGGATGTCCGTGTCTGATCGGTTATTACGAgga               >  7:162097/1‑147 (MQ=37)
                                                                                                                               accccGCGCCTGTAGGAAGGGGTGTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAac           <  8:249434/147‑1 (MQ=255)
                                                                                                                                   cccgcCTGTAGCAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCTCCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGa       <  7:148645/147‑1 (MQ=255)
                                                                                                                                    gcgcCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAgg                <  3:12825/139‑1 (MQ=255)
                                                                                                                                    gcgcCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAgg                >  4:12825/1‑139 (MQ=255)
                                                                                                                                      gcCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCATCCCTCGCTAGGATGGCCGTGTCTGATCGTTTATTACGAGGCAAACACGATGa    <  7:59874/149‑1 (MQ=255)
                                                                                                                                        cTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAAc  <  3:280005/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
CTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAAC  >  NC_002947/1639442‑1639726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑