| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,703,250 | G→C | 18.0% | intergenic (+99/‑68) | lysS → / → PP_1497 | lysine‑‑tRNA ligase/TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,703,250 | 0 | G | C | 18.0% | 107.3 / 23.4 | 56 | intergenic (+99/‑68) | lysS/PP_1497 | lysine‑‑tRNA ligase/TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (25/21); new base C (6/4); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01 | |||||||||||
CGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCC > NC_002947/1703102‑1703393 | cGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAgg > 3:47246/1‑149 (MQ=255) ctCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGAGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTg < 4:234369/148‑1 (MQ=255) aTCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTCGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCtttg < 5:137866/149‑3 (MQ=38) tCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGt < 3:110128/149‑1 (MQ=255) gccGCGACGTGATCCTGAGCGCGCACATGCGTCCGCAGGACTGAGTGAGCTGCACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGATGCTT‑‑TATGTTGTCAGTACCGGCCTGTTCGCGGGTATAGCCGCTCCTAC‑AAGGCCtttgt < 4:170807/148‑4 (MQ=255) cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTGCTGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAg < 7:203881/149‑1 (MQ=38) cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTa < 5:86254/148‑1 (MQ=255) cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCTTTGTAg < 6:240309/149‑1 (MQ=255) gcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAAACCGCTCCGACAAAGGGCTTGTAgg > 7:320444/1‑149 (MQ=255) gcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAACCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAg > 7:371843/1‑148 (MQ=255) cgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGa < 6:318630/149‑1 (MQ=255) gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCgg < 3:174884/149‑1 (MQ=255) ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTAccc < 5:149603/148‑1 (MQ=255) ttCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCcg < 7:20794/149‑1 (MQ=255) ccGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCcgcg > 8:291590/1‑148 (MQ=255) gCACATGCGTCCGCAGGCCTGAGTGAGCGGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGACGCTT‑‑TATGTGGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAg < 5:273197/149‑1 (MQ=255) gTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTGGTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCCCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGc > 1:314539/1‑147 (MQ=255) gTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAg > 1:220018/1‑148 (MQ=255) agCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACAcccc > 5:387472/1‑148 (MQ=255) gCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCAAAAAAGGCCGTGTAGGAGCGGGTTTACCCGCGAAGGGGGCAGCACTGACGAAAcccccc > 8:206639/1‑147 (MQ=38) tGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTAccc < 6:84399/115‑1 (MQ=255) tGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTAccc > 5:84399/1‑115 (MQ=255) gccgccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGGCTTGTAGGCGCGGGTTTTCCCGCGAAGGGGGCCGCACGGACGGACCACCCCCATGAGGGAtg > 8:232850/1‑148 (MQ=37) gccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGt < 3:280421/99‑1 (MQ=255) gccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGt > 4:280421/1‑99 (MQ=255) tCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGt < 6:387472/147‑1 (MQ=255) ggcTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGc < 7:291590/149‑1 (MQ=255) tttttCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCAAGAGGTATACCCCGTGACAccccccct > 3:158561/1‑147 (MQ=255) gCCTTATGCTT‑‑TTTGTTGTCCTTACCGGCCTTTTCGCGGGTAAACCCGCTCATAAAATGGCTTTTTTGGTGGGGTTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTg < 4:158561/146‑1 (MQ=255) gCCTGAAGGTT‑‑TATGTTGTCAGTCCCGGCCTCTTCGCGGTTAAACCCGCTCCTACAATGGCCTTGTTGTTGCGGTTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGAc < 2:314539/148‑1 (MQ=255) gAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTTGGAGCGGGTTTACCAGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGGATCCCCCGTGCCACCCGCCATGCCTGACCACgg > 1:48222/1‑149 (MQ=38) tATGTTGTCAGTCCCGGGGTGTTCGCGGGTAAACCCGCTCCTACAAAGGCGTTGTTGGCGCGGGTTAACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCGCCGTGACACCCGCCATGCCTGACCAAGGcgccgc < 2:48222/148‑1 (MQ=37) aTGTTGTCAGTACCGGCCTCTTCGCGGTTAAACCCGCTCCTACAAAGGCCTTGTCGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCgccgcc < 8:371843/148‑1 (MQ=255) gtCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATACCCCCTGACACCCGCCATGCCGGACCAAGGCGCCGCCGGCATc > 6:181209/1‑149 (MQ=255) tCAGTCCCGGCCTCTTCGCGGTAAAACCCGCTCCTACAACGGCCTTGTTGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCg < 2:220018/149‑1 (MQ=38) cAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGc > 4:271547/1‑149 (MQ=255) cGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCGTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCGCACTGCCGAAACACCCCCATGAGGTTTGCCCCGGGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCAccgc > 1:68086/1‑149 (MQ=37) gCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAACCACCCCCATGAGGTATCCCCCGTGACACCCGCCAGGCCTGACCAAGGCGCCGCCGGCATCGCCAccgccg > 5:397382/1‑149 (MQ=255) ctctTCGCGGGTAAACCCCCACCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCAAGAGGTATCACCCCTGACACCCGCCATGCCAGGCCAACGCGCCGCCGGCAACGCCAccccccgc > 4:54028/1‑146 (MQ=25) ttCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAACCACCCCCATGAGGTATCCCCCGTGACCCCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGcc > 3:287191/1‑149 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGcc > 6:342135/1‑149 (MQ=255) cgGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGa > 3:301583/1‑147 (MQ=255) gggTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGCCCAAGGCGCCGCCGGCATCGCCCCCGCCGGCGCCCAAAg > 3:243729/1‑149 (MQ=255) aaCCGGCTCCTACAATGGCCTTGTCGGAGGGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAATCGTGc < 7:355516/149‑1 (MQ=255) aCCCGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAACACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGc > 7:307643/1‑148 (MQ=255) cccGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGcc < 5:32109/137‑1 (MQ=255) cccGCTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGcc > 6:32109/1‑137 (MQ=255) ccccTCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCACCGTGACACCCGCCATGCCTGACAAAGGCGCCGCCGGCATCGCCACCGCCGTCCCCGAAACCGTGCaa > 2:345878/4‑148 (MQ=37) tCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAcc > 5:129974/1‑148 (MQ=255) tCCTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAcc > 3:184728/1‑148 (MQ=255) ccTACAAAGGCCTTGTAGGCGGGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCaa < 2:68086/149‑1 (MQ=255) cTACATTGGCCTTTTAGTTGCGGTTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGGCGTCGCCGAAAGCGTGCAATACCaa < 5:334780/148‑1 (MQ=255) cTACAAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAg > 6:114706/1‑149 (MQ=255) tACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAgg > 2:329961/1‑149 (MQ=255) aCAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCACCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACAAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCCAGGc > 3:354532/1‑149 (MQ=255) cAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGcc > 3:137052/1‑149 (MQ=255) cAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCAATGCCTGAACAATGATCCTCCGGAATCGCCACCGCCGTCGCAGCAAGCGTTCAATACCAATGcc > 5:220690/1‑149 (MQ=38) | CGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTT‑‑TATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCC > NC_002947/1703102‑1703393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |