Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,704,637	T→A	6.1%	M1* (TTG→TAG) †	PP_1499 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,704,637	0	T	A	6.1%	223.9 / 3.0	76	M1* (TTG→TAG) †	PP_1499	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/39);  new base A (3/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGACAA  >  NC_002947/1704496‑1704784                                                                                                                                              |                                                                                                                                                     cAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCcgcg                                                                                                                                               <  8:109635/149‑1 (MQ=255) cAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCcgc                                                                                                                                                >  3:134149/1‑148 (MQ=255)  aaCGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCTAAGTGGCGCGGGTCCAGCGCTCGGGCAAATGACCGTGGTGGCGTCTTCGCGGGCTTGCCCGCGa                                                                                                                                              <  5:270970/149‑1 (MQ=255)  aaCGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCcgcg                                                                                                                                               >  3:63818/1‑148 (MQ=255)    cggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCcgcg                                                                                                                                               >  6:175313/1‑146 (MQ=255)     ggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGCCCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGaa                                                                                                                                             >  7:197277/1‑147 (MQ=255)     ggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGaa                                                                                                                                             >  7:276826/1‑147 (MQ=255)       ggacggcgTGCTGATCGACAGCCGGGCGGCGGGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGTAGTGGCGCGGTTCCTGCGCTGGGGCAAATGACCGTGTTGGCCTCTTCGCGGGCAAGCCCGCGAAGAgg                                                                                                                                         <  5:45063/148‑1 (MQ=255)       gcacggctTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAgg                                                                                                                                         >  4:94223/3‑149 (MQ=255)                 cTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAgg                                                                                                                               >  5:372151/1‑149 (MQ=255)                     tCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTc                                                                                                                           <  2:268877/149‑1 (MQ=255)                       gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgt                                                                                                                         >  4:356532/1‑149 (MQ=255)                       gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgt                                                                                                                         <  4:63818/149‑1 (MQ=255)                          aGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgtg                                                                                                                        >  3:292614/1‑147 (MQ=255)                             ccGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTa                                                                                                                   <  7:34051/149‑1 (MQ=255)                             ccGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTa                                                                                                                   <  3:51071/149‑1 (MQ=255)                              cggcggCGCGGGGGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGGTCGTCGACGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAg                                                                                                                  <  7:157597/149‑1 (MQ=255)                                gcggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGaa                                                                                                                <  4:292614/149‑1 (MQ=255)                                    cccgGGCGCCGGCCAACCTGCCCGACCCGGCCAACACCGCCCTCCTCGTCGAAGTCGCGCGGCTCCAGCGCTCGGGCAAATGACCGGGCTGGCGCCTTCGCGGGCGTGCCCGCGAAGAGGCCGATACAGGTTTCGTGTACTAGAAAGGa                                                                                                            <  8:357346/147‑1 (MQ=255)                                     gcgGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCACGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAg                                                                                                           >  2:309867/1‑149 (MQ=255)                                       gggTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                              >  2:38649/1‑144 (MQ=255)                                       gggTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                              <  1:38649/144‑1 (MQ=255)                                            cTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCg                                                                                                     <  6:95015/148‑1 (MQ=255)                                              ggCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                                  >  8:308971/1‑149 (MQ=255)                                               gCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGt                                                                                                   <  6:297382/147‑1 (MQ=255)                                               gCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                                  <  5:175313/148‑1 (MQ=255)                                                 cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                               <  7:197172/149‑1 (MQ=255)                                                 cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                               <  4:134149/149‑1 (MQ=255)                                                 cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                               <  2:201167/149‑1 (MQ=255)                                                 cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                               >  8:15343/1‑149 (MQ=255)                                                 cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGAGCAAATGACCGTGTTGGGGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                               <  8:276826/149‑1 (MQ=255)                                                   aCCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGGTCCCGGTGGACg                                                                                              >  3:184359/1‑148 (MQ=255)                                                    ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGaa                                                                                            <  7:308971/149‑1 (MQ=255)                                                    ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGaa                                                                                            >  2:82000/1‑149 (MQ=255)                                                     cTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGaa                                                                                            >  7:136326/1‑148 (MQ=255)                                                      tGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGAagac                                                                                                                                          >  6:109653/1‑100 (MQ=255)                                                      tGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGAagac                                                                                                                                          <  5:109653/101‑2 (MQ=255)                                                          cgacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAg                                                                                     <  3:81376/149‑1 (MQ=255)                                                            acCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCa                                                                                   <  3:94223/149‑1 (MQ=255)                                                             cccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑Ac                                                                                        <  2:73875/143‑1 (MQ=255)                                                             cccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑Ac                                                                                        >  1:73875/1‑143 (MQ=255)                                                                 gCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                              <  4:92080/118‑1 (MQ=255)                                                                 gCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                              >  3:92080/1‑118 (MQ=255)                                                                    aaCTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATcc                                                                           <  3:356532/149‑1 (MQ=255)                                                                       tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCcgcg                                                                        >  7:200522/1‑149 (MQ=255)                                                                         cGACTTGTTCGTCGATGTGGCGCGGTTCCAGCGCTCGGGCAAATGACAGTGTTGGCGGCTTCGCGGGCTTTCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGcc                                                                      <  4:160949/149‑1 (MQ=255)                                                                         cGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGcc                                                                      <  7:15343/149‑1 (MQ=255)                                                                               gTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTcc                                                                >  7:224947/1‑149 (MQ=255)                                                                                ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTc                                                                 <  8:165599/147‑1 (MQ=255)                                                                                   gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGAcc                                                            >  8:118281/1‑149 (MQ=255)                                                                                    tcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACTGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGGTTAGTGTTGTAGTAAGGAGTTCCCGGTGGGCGAAT‑ACCAGCCAACCATCCGCGCCCTGTCCGACCg                                                           >  4:376597/1‑149 (MQ=38)                                                                                    tcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCg                                                           >  4:363234/1‑149 (MQ=255)                                                                                      gAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTAGCGGGCTTGCCCGCGAAGAGGCCGGTAAAGGTTTAGTGTACTAGAAAGGAGTTACCGGTTGACGAAT‑ACCAGCAAACCATAAGCGCCCTTTCCGACCGc                                                          >  4:192841/1‑148 (MQ=255)                                                                                       aaGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCAt                                                        <  8:197277/149‑1 (MQ=255)                                                                                         gTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTATCCGACCGCAtcg                                                      <  3:192841/149‑1 (MQ=255)                                                                                              gcgGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                                 >  6:153115/1‑149 (MQ=255)                                                                                              gcgGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                                 <  8:224947/149‑1 (MQ=255)                                                                                                    ccAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGca                                              >  1:265281/1‑146 (MQ=255)                                                                                                     cAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAg                                           <  2:265281/148‑1 (MQ=255)                                                                                                      aGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTAGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGAc                                         >  7:245827/1‑149 (MQ=255)                                                                                                       gcgcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACg                                        >  8:30145/1‑149 (MQ=255)                                                                                                        cgcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                       <  8:136326/149‑1 (MQ=255)                                                                                                              ggCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATcc                                 <  6:345855/149‑1 (MQ=255)                                                                                                                 aaaTGACCGTGTTGGCGTCTTGGGGGGCTTGGCCGGTAATTGGCCGGTACAGGTTTGGTGTACTTGAAAGGTGTTCCCGGTGGACGATTCACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                               <  1:309867/149‑1 (MQ=255)                                                                                                                 aaaTGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                                 <  7:15244/130‑1 (MQ=255)                                                                                                                 aaaTGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                                 >  8:15244/1‑130 (MQ=255)                                                                                                                        cGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTACCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTg                        >  6:288872/1‑148 (MQ=255)                                                                                                                              ggCGTCTTCGCGGGTTTGCCGGCTAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCgg                  <  2:352128/148‑1 (MQ=255)                                                                                                                                  tCTTCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAg             >  2:11658/1‑149 (MQ=255)                                                                                                                                    ttCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTg           >  3:150945/1‑149 (MQ=255)                                                                                                                                    ttCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTg           >  3:319911/1‑149 (MQ=255)                                                                                                                                      cgcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg          <  8:245827/148‑1 (MQ=255)                                                                                                                                       gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGGCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGga        <  1:82000/149‑1 (MQ=255)                                                                                                                                       gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAAGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGga        >  1:285182/1‑149 (MQ=255)                                                                                                                                        cgGGCTTGCCCGCTAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgac       <  8:200522/149‑1 (MQ=255)                                                                                                                                           gCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCCGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgacgac    <  5:288872/149‑1 (MQ=255)                                                                                                                                             ttGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGacaa  >  3:261207/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                     CAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAT‑ACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGACAA  >  NC_002947/1704496‑1704784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑