Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,728,303 A→T 5.7% V24E (GTG→GAG)  PP_1521 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,728,3030AT 5.7% 209.5 / 2.7 70V24E (GTG→GAG) PP_1521hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/34);  new base T (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

GTCATGGGGTGTGTGCCCTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTCAACGATGC  >  NC_002947/1728159‑1728440
                                                                                                                                                |                                                                                                                                         
gTCATGGGGTGTGTGCCCTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACtt                                                                                                                                        >  5:107212/1‑148 (MQ=255)
   aTGGGGTGTGTGCCCTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGAGTCATATACTCGGCCTGCCACTTGCAg                                                                                                                                    <  4:212815/149‑1 (MQ=255)
     ggggTGTGTGCCCTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGa                                                                                                                                  >  3:381408/1‑149 (MQ=255)
      gggTGTGTGCCCTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGa                                                                                                                                  <  6:22813/148‑1 (MQ=255)
         tgtgtgCCCTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATAtt                                                                                                                              <  1:353161/149‑1 (MQ=255)
                ccTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGc                                                                                                                         >  2:282733/1‑147 (MQ=255)
                 cTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAg                                                                                                                      <  7:354600/149‑1 (MQ=255)
                      tgtgATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGGAAAATTCGGCCAACGAAGGGACAGTCAGGATTTTTTTTTGTTCTGTGTCATATACTCGGCGTGCCACTTGCAGGATATTTCGGCatggggag                                                                                                                 >  2:361171/1‑142 (MQ=255)
                      tgtgATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAgcggcg                                                                                                                 >  3:117486/1‑149 (MQ=255)
                        tgATTTTCAGGTGAGTATTCTGCTATTTTGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGACGtt                                                                                                               >  6:369559/1‑149 (MQ=255)
                                    gAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCa                                                                                                                                     <  2:231950/115‑1 (MQ=255)
                                    gAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCa                                                                                                                                     >  1:231950/1‑115 (MQ=255)
                                     aGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATCTCGGCAAGCGGCGTTGGCACGGGGCAg                                                                                                   <  7:138074/148‑1 (MQ=255)
                                       tATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGTCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGt                                                                                                 <  2:290792/148‑1 (MQ=255)
                                        aTTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGa                                                                                               >  4:318051/1‑149 (MQ=255)
                                            tGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCGTCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTc                                                                                           >  1:362123/1‑149 (MQ=255)
                                            tGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTc                                                                                           >  6:197384/1‑149 (MQ=255)
                                              cTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATTCTCGGCCTTCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTc                                                                                           <  5:272558/147‑1 (MQ=255)
                                              cTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGt                                                                                         <  1:291821/149‑1 (MQ=255)
                                               tATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCTTCATAAATTTTTTTTGTTCTGTGTCAAATACTCCGCCTGCCACTTGAAGGAAATTTCGTAAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTg                                                                                        >  8:216303/1‑149 (MQ=255)
                                                 tttAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGat                                                                                                                                 >  3:78015/1‑106 (MQ=255)
                                                 tttAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGcc                                                                                      >  6:281359/1‑149 (MQ=255)
                                                 tttAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGcc                                                                                      >  1:264975/1‑149 (MQ=255)
                                                 tttAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGc                                                                                       <  3:318051/148‑1 (MQ=255)
                                                 tttAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGGCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGat                                                                                                                                 <  4:78015/106‑1 (MQ=255)
                                                   tAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCGTCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCt                                                                                     <  2:362123/148‑1 (MQ=255)
                                                     gTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGc                                                                                   >  6:309608/1‑148 (MQ=255)
                                                      tCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCaa                                                                                 <  7:216303/149‑1 (MQ=255)
                                                        tGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGTCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAgg                                                                               >  2:82121/1‑149 (MQ=255)
                                                          ccGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGc                                                                              >  8:170022/1‑148 (MQ=255)
                                                          ccGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCg                                                                             <  6:107212/149‑1 (MQ=255)
                                                           cGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGACGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGa                                                                            <  5:369559/149‑1 (MQ=255)
                                                                 ggCGTAAGTTCAGCAACATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTc                                                                       <  6:6526/148‑1 (MQ=255)
                                                                 ggCGTAAGTTCAGCAAACTTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTca                                                                      <  2:334360/149‑1 (MQ=255)
                                                                 ggCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTc                                                                       <  5:106634/148‑1 (MQ=255)
                                                                    gTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAA‑TTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACaa                                                                   >  8:185928/1‑148 (MQ=255)
                                                                      aaGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCa                                                                                                    <  8:187794/114‑1 (MQ=255)
                                                                      aaGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCa                                                                                                    >  7:187794/1‑114 (MQ=255)
                                                                         ttCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATaaa                                                               >  7:220014/1‑148 (MQ=255)
                                                                            aGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATc                                                           >  1:40160/1‑149 (MQ=255)
                                                                             ggaaaaTTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATcc                                                          >  6:73201/3‑149 (MQ=255)
                                                                              cAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCg                                                         <  4:381408/149‑1 (MQ=255)
                                                                               aaaaTTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCgg                                                        <  2:40160/149‑1 (MQ=255)
                                                                                aaaTTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGa                                                       <  5:197384/149‑1 (MQ=255)
                                                                                aaaTTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCGCGTGCCTGCAAGGCGATCTTCACCATAAAAATCCGGa                                                       <  4:117486/149‑1 (MQ=255)
                                                                                 aaTTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCTCTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGag                                                      >  4:267236/1‑149 (MQ=255)
                                                                                 aaTTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCTCTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGag                                                      >  4:219182/1‑149 (MQ=255)
                                                                                 aaTTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGTCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGag                                                      <  5:232227/149‑1 (MQ=255)
                                                                                        ccAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCTCTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCa                                               <  3:267236/149‑1 (MQ=255)
                                                                                        ccAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCTCTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCa                                               <  3:219182/149‑1 (MQ=255)
                                                                                                     cGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGt                                  <  5:309608/149‑1 (MQ=255)
                                                                                                       tcatcaATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTga                                <  7:170022/149‑1 (MQ=255)
                                                                                                          tcaATTTTTTTTGTTCTGTGTCGTATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATgtc                                         >  7:248910/1‑137 (MQ=255)
                                                                                                          tcaATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATgtc                                         <  8:248910/137‑1 (MQ=255)
                                                                                                           tcaatttttttGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACt                            <  7:185928/145‑1 (MQ=255)
                                                                                                             aTTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTgg                          <  5:281359/149‑1 (MQ=255)
                                                                                                              ttttttttGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTgg                          >  7:346111/1‑148 (MQ=255)
                                                                                                                 tttttGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGaa                       <  5:73201/148‑1 (MQ=255)
                                                                                                                         cTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGt               <  4:16562/148‑1 (MQ=255)
                                                                                                                            tgtCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGt            >  2:75145/1‑148 (MQ=255)
                                                                                                                            tgtCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGt            <  1:282733/148‑1 (MQ=255)
                                                                                                                              taatatACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGCGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTc          >  6:109207/3‑148 (MQ=255)
                                                                                                                                atatACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTCAAc       >  1:187753/1‑149 (MQ=255)
                                                                                                                                 tataCTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTCAAc       >  4:339233/1‑148 (MQ=255)
                                                                                                                                    aCTCGGCCTGCCACTTTCAGGATATTTAGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAAGAAATATCCGGAGTAGTTCAGAGGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTCAACGATg   >  2:1456/1‑149 (MQ=255)
                                                                                                                                      tCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAAt                      <  4:79294/128‑1 (MQ=255)
                                                                                                                                      tCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAAt                      >  3:79294/1‑128 (MQ=255)
                                                                                                                                      tCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTCAACGAt    <  6:124167/146‑1 (MQ=255)
                                                                                                                                      tCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTCAACGAt    >  5:124167/1‑146 (MQ=255)
                                                                                                                                      tCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTCAACGATGc  >  4:278945/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                         
GTCATGGGGTGTGTGCCCTGGGTGTGATTTTCAGGTGAGTATTCTGCTATTTAGTCTGCCGTATCGGCGTAAGTTCAGCAAAATTCGGCCAACGACGGGACCGTCATCAATTTTTTTTGTTCTGTGTCATATACTCGGCCTGCCACTTGCAGGATATTTCGGCAAGCGGCGTTGGCACGGGGCAGGTGACCTCGTGCCTGCAAGGCGATCTTCACAATAAAAATCCGGAGTAGTTCAGATGTCGTCTCGTGAGACTGGGAATGTAAAGTGGTTCAACGATGC  >  NC_002947/1728159‑1728440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑