Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,797,596 T→A 9.0% V99V (GTT→GTA)  lpxA → acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,797,5960TA 9.0% 173.7 / 5.5 63V99V (GTT→GTA) lpxAacyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (26/30);  new base A (3/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTGGGTGACTGGGCGATC  >  NC_002947/1797450‑1797743
                                                                                                                                                    |                                                                                                                                                   
cGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCa                                                                                                                                                   <  3:364812/149‑1 (MQ=255)
   aTCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGa                                                                                                                                                <  7:155893/149‑1 (MQ=255)
         aaGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCGGGACCTTACGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGAGCACATTGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACcgcgc                                                                                                                                          <  7:32275/149‑1 (MQ=255)
         aaGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTCCTGAACAGTACcgcgg                                                                                                                                          <  1:206253/149‑2 (MQ=255)
         aaGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTCCTGAACAGTACcgcgg                                                                                                                                          >  2:206253/1‑148 (MQ=255)
         aaGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACcgcgc                                                                                                                                          >  8:134647/1‑149 (MQ=255)
         aaGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACcgcgc                                                                                                                                          <  2:322242/149‑1 (MQ=255)
          aGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTCCTGAACAGTACcgcggt                                                                                                                                         <  4:355688/149‑3 (MQ=255)
             accaCCGTATCTTTCAGTATTCCTCGATCGGTGAACACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGAGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGaa                                                                                                                                      <  8:156425/146‑1 (MQ=255)
             acaACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGaa                                                                                                                                      <  7:358300/149‑1 (MQ=255)
              caACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGaaa                                                                                                                                     <  6:118823/149‑1 (MQ=255)
                    tATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACGAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCg                                                                                                                               >  3:305816/1‑149 (MQ=255)
                      tCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGACCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGt                                                                                                                              <  2:178267/148‑1 (MQ=255)
                         ttCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGt                                                                                                                          >  2:292801/1‑149 (MQ=255)
                          tCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTgg                                                                                                                            <  5:168884/146‑1 (MQ=255)
                          tCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGCTCAGGACCGCGCGGAAACCACCGTGGGt                                                                                                                          <  7:371788/148‑1 (MQ=255)
                            aGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGa                                                                                                                        <  5:267138/148‑1 (MQ=255)
                                    tCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCt                                                                                                               >  6:104768/1‑149 (MQ=255)
                                      gATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAAc                                                                                                                 >  3:145322/1‑145 (MQ=255)
                                      gATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAAc                                                                                                                 <  4:145322/145‑1 (MQ=255)
                                           gTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATg                                                                                                        <  1:292801/149‑1 (MQ=255)
                                           gTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATAGACGAAAGGGGGGCCCAAACCCCCCGGGTAGTGTACGGGACCGCG‑GGAAACCCACGGGGGGGCCCAAAAccattaaat                                                                                                         >  1:319192/1‑142 (MQ=255)
                                           gTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTCATTCGCG‑AAGGGGTGACA‑ATCCACCGCGGTACGGTTCAGGACCGCGCGGAAACCACCGAGGGTGACCATAACCTCATCATg                                                                                                        >  8:170183/1‑149 (MQ=255)
                                              aaGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGc                                                                                                      <  7:344600/148‑1 (MQ=255)
                                                  cacCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATg                                                                                                 <  1:1676/149‑1 (MQ=255)
                                                     cccGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGcc                                                                                               >  8:159747/1‑148 (MQ=255)
                                                     cccGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGcc                                                                                               >  5:39181/1‑148 (MQ=255)
                                                     cccGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTACAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGcc                                                                                               >  8:155859/1‑148 (MQ=255)
                                                          aCCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACAtt                                                                                         >  4:130338/1‑149 (MQ=255)
                                                            cTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTgg                                                                                       >  5:303236/1‑149 (MQ=255)
                                                             ttAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTg                                                                                                                             >  8:202957/1‑110 (MQ=255)
                                                             ttAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTg                                                                                                                             <  7:202957/110‑1 (MQ=255)
                                                                   aCAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGa                                                                                 >  8:159642/1‑148 (MQ=255)
                                                                    cAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACa                                                                               <  5:104768/149‑1 (MQ=255)
                                                                            aGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATc                                                                       <  7:134647/149‑1 (MQ=255)
                                                                               cGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTc                                                                          >  3:247427/1‑143 (MQ=255)
                                                                               cGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTc                                                                          <  4:247427/143‑1 (MQ=255)
                                                                               cGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCgg                                                                     >  6:331260/1‑148 (MQ=255)
                                                                                  cgcgCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTACAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAAc                                                                 <  7:155859/149‑1 (MQ=255)
                                                                                      ccTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACt                                                             <  2:323565/149‑1 (MQ=255)
                                                                                                 gCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGt                                                   >  3:17922/1‑148 (MQ=255)
                                                                                                    aTCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGt                                                   <  4:74015/145‑1 (MQ=255)
                                                                                                    aTCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGt                                                   >  3:74015/1‑145 (MQ=255)
                                                                                                    aTCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGAACGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTcaa                                                >  1:368098/1‑148 (MQ=255)
                                                                                                     tCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTGCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTcaac                                               <  4:14240/148‑1 (MQ=255)
                                                                                                          aTGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACg                                         <  7:159747/149‑1 (MQ=255)
                                                                                                           tgtgATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACgg                                        >  8:40939/1‑149 (MQ=255)
                                                                                                                ttCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCGACAACACGGCGTTg                                   >  8:190152/1‑149 (MQ=255)
                                                                                                                   gcg‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTgg                                  >  7:137811/1‑147 (MQ=255)
                                                                                                                   gcg‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCa                                <  2:368098/149‑1 (MQ=255)
                                                                                                                    cg‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAg                               <  4:17922/149‑1 (MQ=255)
                                                                                                                    cg‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAg                               <  5:331260/149‑1 (MQ=255)
                                                                                                                    cg‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAg                               >  4:219629/1‑149 (MQ=255)
                                                                                                                        aGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACt                                                             >  4:167365/1‑116 (MQ=255)
                                                                                                                        aGGGGTCACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACt                                                             <  3:167365/116‑1 (MQ=255)
                                                                                                                           ggTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATg                         <  6:303236/149‑1 (MQ=255)
                                                                                                                                   aTCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACAtgt                  >  5:285337/1‑149 (MQ=255)
                                                                                                                                     ccACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTgg                >  7:296807/1‑149 (MQ=255)
                                                                                                                                      cACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTgg                <  7:40939/148‑1 (MQ=255)
                                                                                                                                      cACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTgg                <  2:319192/148‑1 (MQ=255)
                                                                                                                                              tACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTGGGTGACTggg       >  5:139987/1‑149 (MQ=255)
                                                                                                                                              tACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTGGGTGACTggg       <  3:130338/149‑1 (MQ=255)
                                                                                                                                               aCTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTGGCTGACTGGGc      >  3:375189/1‑149 (MQ=255)
                                                                                                                                                   ttCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCCACAACACGGCGTTGGCAGGTCATGTACATGTGGGTGACTGGGCGATc  <  7:190152/149‑1 (MQ=255)
                                                                                                                                                   ttCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTGGGTGACTGGGCGATc  >  4:184498/1‑149 (MQ=255)
                                                                                                                                                   ttCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTGGGTGACTGGGCGATc  >  8:186239/1‑149 (MQ=255)
                                                                                                                                                   ttCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTGGGTGACTGGGCGATc  <  6:285337/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGATCGGTGAAGACACCCCGGACCTTAAGTACAAGGGTGAGCCGACGCGCCTGGTGATCGGCGATCACAATGTGATTCGCG‑AAGGGGTGACC‑ATCCACCGCGGTACTGTTCAGGACCGCGCGGAAACCACCGTGGGTGACCATAACCTGATCATGGCCTATGCCCACATTGGCCACGACAGTGTCATCGGCAACCACTGCATCCTGGTCAACAACACGGCGTTGGCAGGTCATGTACATGTGGGTGACTGGGCGATC  >  NC_002947/1797450‑1797743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑