Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,892,225 C→A 6.3% P156Q (CCG→CAG)  PP_1697 → transcriptional regulator, GntR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,892,2250CA 6.3% 256.9 / 5.4 79P156Q (CCG→CAG) PP_1697transcriptional regulator, GntR family
Reads supporting (aligned to +/- strand):  ref base C (39/35);  new base A (3/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

ATCGAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACTGCCGGCC  >  NC_002947/1892077‑1892373
                                                                                                                                                    |                                                                                                                                                    
aTCGAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGcc                                                                                                                                                      >  3:215836/1‑149 (MQ=255)
     gttgCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCgctgc                                                                                                                                                 >  1:260124/4‑149 (MQ=255)
      tCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCgctgct                                                                                                                                                >  6:360482/1‑149 (MQ=255)
       cGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGctgctg                                                                                                                                               <  4:174857/149‑1 (MQ=255)
        gCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGctgctg                                                                                                                                               >  7:69777/1‑148 (MQ=255)
            ccGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGAc                                                                                                                                            <  7:270275/147‑1 (MQ=255)
            ccGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGt                                                                                                                                          <  8:91017/149‑1 (MQ=255)
            ccGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGt                                                                                                                                          <  6:215106/149‑1 (MQ=255)
            ccGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCAGCGGCTGGatgg                                                                                                                                          <  2:231024/149‑4 (MQ=255)
            ccGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGAGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGt                                                                                                                                          >  5:385125/1‑149 (MQ=255)
                 gcCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATc                                                                                                                                     <  6:385125/149‑1 (MQ=255)
                    ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTg                                                                                                                                   >  7:323892/1‑148 (MQ=255)
                    ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCAGCGGCTGGaag                                                                                                                                           >  3:48280/1‑138 (MQ=255)
                    ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCAGCGGCTGGaag                                                                                                                                           <  4:48280/140‑3 (MQ=255)
                        gcgGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAg                                                                                                                              >  1:363773/1‑149 (MQ=255)
                               ccGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGc                                                                                                                       <  2:231047/149‑1 (MQ=255)
                                cGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATGATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGTTGCTGGACGTGGATCTGAACAGCGAGGGcc                                                                                                                      >  4:100338/1‑149 (MQ=255)
                                     cATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctg                                                                                                                 <  2:26207/149‑1 (MQ=255)
                                      aTTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctgc                                                                                                                >  1:77810/1‑149 (MQ=255)
                                      aTTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctgc                                                                                                                >  7:147095/1‑149 (MQ=255)
                                      aTTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctgc                                                                                                                <  8:73252/149‑1 (MQ=255)
                                         gggCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctg                                                                                                                 >  5:73469/1‑145 (MQ=255)
                                         gggCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctg                                                                                                                 <  6:73469/145‑1 (MQ=255)
                                           gCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTAc                                                                                                           >  1:25463/1‑149 (MQ=255)
                                               ggCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGTCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGa                                                                                                       <  3:152493/149‑1 (MQ=255)
                                                  aGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATc                                                                                                     >  4:92644/1‑148 (MQ=255)
                                                  aGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCt                                                                                                    <  8:69777/149‑1 (MQ=255)
                                                     ggAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCt                                                                                                                                    >  6:32235/1‑114 (MQ=255)
                                                     ggAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCt                                                                                                                                    <  5:32235/114‑1 (MQ=255)
                                                                 gATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCg                                                                                     >  4:14555/1‑149 (MQ=255)
                                                                     cGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGACGCGCTGTGCGGTCa                                                                                 <  2:25463/149‑1 (MQ=255)
                                                                      gCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAg                                                                                <  2:260124/149‑1 (MQ=255)
                                                                      gCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAg                                                                                <  8:325444/149‑1 (MQ=255)
                                                                         tcatTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAggtg                                                                             <  5:278598/149‑1 (MQ=255)
                                                                            ttCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTc                                                                          >  5:208228/1‑149 (MQ=255)
                                                                            ttCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTc                                                                          <  8:111512/149‑1 (MQ=255)
                                                                            ttCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTc                                                                          >  3:296662/1‑149 (MQ=255)
                                                                              cGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGc                                                                        >  7:69189/1‑149 (MQ=255)
                                                                                cacaCGTCTGCTCGGCGCCCAGCCAGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGcc                                                                       >  6:252104/1‑148 (MQ=255)
                                                                                 acacGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTa                                                                     >  2:38591/1‑149 (MQ=255)
                                                                                   acGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACg                                                                   >  2:85961/1‑149 (MQ=255)
                                                                                      tCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCg                                                                <  6:208228/149‑1 (MQ=255)
                                                                                          cTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCgaaga                                                            <  3:14555/149‑1 (MQ=255)
                                                                                          cTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCgaa                                                              <  4:215836/147‑1 (MQ=255)
                                                                                           tCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCgaagaa                                                           >  4:20599/1‑149 (MQ=255)
                                                                                              gcgcCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                                        <  3:92644/149‑1 (MQ=255)
                                                                                                         gTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGAccgccg                                              >  4:202132/1‑148 (MQ=255)
                                                                                                            cTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAg                                           <  1:38591/148‑1 (MQ=255)
                                                                                                               gCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGTTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGt                                       <  3:100338/149‑1 (MQ=255)
                                                                                                               gCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGt                                       >  2:127163/1‑149 (MQ=255)
                                                                                                                 cGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTc                                      >  5:10487/1‑148 (MQ=255)
                                                                                                                  gaagaaATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAg                                                                                <  1:14/105‑1 (MQ=255)
                                                                                                                  gaagaaATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAg                                                                                >  2:14/1‑105 (MQ=255)
                                                                                                                       aaTCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCgg                               <  5:360482/149‑1 (MQ=255)
                                                                                                                       aaTCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCgg                               <  2:317669/149‑1 (MQ=255)
                                                                                                                       aaTCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCgg                               <  2:77810/149‑1 (MQ=255)
                                                                                                                        aTCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGt                              <  8:323892/149‑1 (MQ=255)
                                                                                                                           tGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGtc                             >  1:338698/1‑146 (MQ=255)
                                                                                                                             gcggCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTTCATg                         <  7:184126/146‑1 (MQ=255)
                                                                                                                             gCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGACCAGCGAGCGCCCGCGGCTGTACCCGAGCTACGACGCGCTGTGCGGTCAGGTGGGCGCCTACGCCGAACAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCAt                          <  2:363773/148‑1 (MQ=255)
                                                                                                                             gCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATg                         >  8:275343/1‑149 (MQ=255)
                                                                                                                               tGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGc                       >  1:161716/1‑149 (MQ=255)
                                                                                                                               tGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGc                       >  1:189129/1‑149 (MQ=255)
                                                                                                                                   gcgcCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCt                   >  4:164342/1‑149 (MQ=255)
                                                                                                                                     gcCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtg                 >  7:69649/1‑149 (MQ=255)
                                                                                                                                      ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGAcgcg                                <  3:128803/133‑1 (MQ=255)
                                                                                                                                      ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGAcgcg                                >  4:128803/1‑133 (MQ=255)
                                                                                                                                      ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgc                <  8:69189/149‑1 (MQ=255)
                                                                                                                                       cAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTga                            <  3:139611/136‑2 (MQ=255)
                                                                                                                                       cAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTga                            >  4:139611/1‑135 (MQ=255)
                                                                                                                                        aaaCCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCAt                          >  6:340222/1‑137 (MQ=255)
                                                                                                                                        aaaCCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCAt                          <  5:340222/137‑1 (MQ=255)
                                                                                                                                         ccacgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgctg              >  5:93578/6‑148 (MQ=255)
                                                                                                                                         aaCCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgctg              >  4:193366/1‑148 (MQ=255)
                                                                                                                                             ttCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACt         >  7:325778/1‑149 (MQ=255)
                                                                                                                                             ttCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGCCCCGCTGCTGTACCCGATCTACGAAGCGCAGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCCGTGCATGGCCGCTTCCTGCAACt         >  3:348281/1‑149 (MQ=255)
                                                                                                                                             gtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACt         >  6:239522/2‑149 (MQ=255)
                                                                                                                                               ccAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                                        >  8:176800/1‑100 (MQ=255)
                                                                                                                                               ccAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                                        <  7:176800/100‑1 (MQ=255)
                                                                                                                                                  gCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCCTGGCCGCTAGCTGCAccggccgg    >  6:269775/1‑149 (MQ=255)
                                                                                                                                                   ccGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACTgccggc   >  2:184271/1‑149 (MQ=255)
                                                                                                                                                    cGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACTgccggcc  <  3:202132/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCGAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACTGCCGGCC  >  NC_002947/1892077‑1892373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑