Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,063,201	G→A	17.2%	intergenic (+58/‑149)	PP_1838 → / → PP_1839	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,063,201	0	G	A	17.2%	128.4 / 17.5	58	intergenic (+58/‑149)	PP_1838/PP_1839	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/28);  new base A (4/6);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

AATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTT  >  NC_002947/2063053‑2063347                                                                                                                                                      |                                                                                                                                                     aaTGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAg                                                                                                                                                      <  3:35134/149‑1 (MQ=255)      tCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGtcc                                                                                                                                                 >  6:211340/1‑147 (MQ=38)          gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGAc                                                                                                                                             >  4:10975/1‑149 (MQ=255)             gCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                          <  2:319805/149‑1 (MQ=255)              cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCCTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                          <  1:247118/149‑1 (MQ=255)              cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGACTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                          <  1:241600/149‑1 (MQ=255)              cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTtg                                                                                                                                         <  7:142111/149‑1 (MQ=255)               tGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTtgt                                                                                                                                        >  3:85425/1‑149 (MQ=255)                  taGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGGGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑c                                                                                                                                     <  6:314088/147‑1 (MQ=255)                   aGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAg                                                                                                                                   <  3:10975/149‑1 (MQ=255)                    gCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑Caat                                                                                                                                  >  6:359465/1‑147 (MQ=25)                           tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCtgtg                                                                                                                           <  4:345335/149‑1 (MQ=255)                             cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGc                                                                                                                          <  3:174125/148‑1 (MQ=255)                              aGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGa                                                                                                                        <  5:211340/149‑1 (MQ=18)                               gACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAg                                                                                                                       <  5:359465/149‑1 (MQ=18)                                aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGc                                                                                                                      >  1:274780/1‑149 (MQ=255)                                aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGc                                                                                                                      <  5:384026/149‑1 (MQ=255)                                  cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCgg                                                                                                                    <  2:386717/149‑1 (MQ=18)                                  cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCgg                                                                                                                    <  2:274780/149‑1 (MQ=255)                                  cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCgg                                                                                                                    <  3:202096/149‑1 (MQ=255)                                  cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CGCCCACAGGCATTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCgg                                                                                                                    <  8:123269/149‑1 (MQ=25)                                    cgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCG‑CTGCCACAGGGATTGCACAGGGTTCGGACCATGTT‑CACGTACTGTTCGAGCagg                                                                                                                   >  4:116361/1‑148 (MQ=255)                                            agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAACCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                                          <  5:71112/149‑1 (MQ=255)                                             gCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                                          >  6:127688/1‑148 (MQ=255)                                              ccTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCGTGTG‑CAGGTCCTGTGCGCGCGGGCCAGCCcgcg                                                                                                         >  5:6204/1‑148 (MQ=255)                                                 gctgGATACCACCGCCGGCGTGGGGCACGGCACACCGAATTACACCGGCGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGCCAGTGCACAGGGTTCGGCCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                     <  1:30098/149‑1 (MQ=37)                                                 gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTGCCACAGGCATTTCACAGGGTTCGGACCTTGTG‑CAGGTCCTTTGCGAGCGGGCAAGCCCGCGAATg                                                                                                     >  8:182584/1‑149 (MQ=255)                                                 gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGCAAACCGGGGCCGCGTGGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACCGCCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                     <  3:18942/149‑1 (MQ=255)                                                  ctgGATAACCCCGCCGGCGTGGTGCACGGAACACCGAAGTAACCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACCGGGTTCGGACCTTGTG‑CAGGTCCTGTGCTAGCGGGCAAGCCCGCGAATg                                                                                                     <  6:6204/148‑1 (MQ=38)                                                  ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCCCAGGCATTGGACAGGGGTGGGCCCTTGTG‑CAGGGCCGGTGCGAGCGGGCTAGCCCGCGAATg                                                                                                     >  2:30098/1‑148 (MQ=38)                                                  ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                     <  5:127688/148‑1 (MQ=255)                                                     gATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATGCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATgg                                                                                                    <  1:251588/147‑1 (MQ=255)                                                          cacCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGACCG‑CTCCCACAGGCATTGCACAGGGTTAGGACCTTGTG‑GAGGTCCTGTGCGAGCCGGCAAGCCCGCCAAAGGGCCCCGcc                                                                                            >  6:380169/1‑148 (MQ=255)                                                           acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCG‑CTCCCCCAGGGCTTGGACAGGGTTCGGGCCTTGTG‑GAGGGGCGGTGGGCGCGGGCCAGCCCGCGGATGGGGCCCGcc                                                                                            >  1:239375/1‑147 (MQ=255)                                                                  gCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTc                                                                                    <  4:85425/149‑1 (MQ=255)                                                                         gCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCAAAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                             >  2:107453/1‑149 (MQ=255)                                                                          cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTCCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTg                                                                            <  5:380169/149‑1 (MQ=255)                                                                          cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CACCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                             >  6:311707/1‑148 (MQ=255)                                                                                  acCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACACGCAGTGCACAGGGGTCGGCCCTTGTG‑CCGGGCCTGGGCGAGCGGGCACGCCCGCGAATGGGGCCCGCCCAAGTTTCCCGGCCGG‑ACCAGTAt                                                                   >  7:126387/1‑149 (MQ=25)                                                                                     gAAGTAACCGGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CGCCCACAGCCATTGCACCGGGTGCGCCCCTTGTG‑CAGGTCCTGGGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATccc                                                                <  8:213814/149‑1 (MQ=37)                                                                                                gggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGt                                                     <  5:311707/149‑1 (MQ=255)                                                                                                gggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGt                                                     <  7:182584/149‑1 (MQ=255)                                                                                                  cccGCGTTGCGGCCCATTCGCGGGCGTGCCCG‑CTCCCACAGGCATGCCACAGGGTGCGCCCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATTCCCGCCCCAAGGTCa                                                   <  6:301386/148‑1 (MQ=38)                                                                                                  cccGCGTGGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGCACCTTGTC‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa                                                                               <  7:120849/121‑1 (MQ=255)                                                                                                                  ttCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGCCTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcac                                   >  5:140953/1‑149 (MQ=14)                                                                                                                     gcgGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcacaca                                >  1:354211/1‑149 (MQ=255)                                                                                                                           ttGCCCG‑CTCCCACAGGCTTTGCACTGGGTGCGGACGTTGTG‑CAGGTCGTGTGCGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgt                          <  5:206649/149‑1 (MQ=255)                                                                                                                             gCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt                        >  8:206854/1‑149 (MQ=255)                                                                                                                               ccG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCa                                           >  2:153087/1‑128 (MQ=14)                                                                                                                               ccG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCa                                           <  1:153087/128‑1 (MQ=14)                                                                                                                                 g‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                    <  2:239375/149‑1 (MQ=255)                                                                                                                                   cTCCCATAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGCAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                    <  1:107453/149‑1 (MQ=255)                                                                                                                                       cacaGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt               >  8:107256/1‑149 (MQ=255)                                                                                                                                         caGGCCCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga             <  6:140953/149‑1 (MQ=21)                                                                                                                                          aGGCATTGCACCGGGTTCGGACCTTGTG‑CTGGTCCTGTGCGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag            <  2:354211/149‑1 (MQ=255)                                                                                                                                          aGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag            >  5:43139/1‑149 (MQ=255)                                                                                                                                                tGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTc      >  6:68140/1‑149 (MQ=255)                                                                                                                                                  cacaGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCCGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGTTCCg    >  6:215879/1‑149 (MQ=255)                                                                                                                                                   acaGGGTTCGGACCTTCTG‑CAGGTCCTGACCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAAATTTCCCGGACTG‑AACATTAACCCCGCCCCAAGGTCAGTTTTTCAGGCATCACACACCAGGTGTACGTTGACAGAGAGCGTCCGt   >  2:390978/1‑149 (MQ=255)                                                                                                                                                    ctgggTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATTGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt  <  3:116361/147‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     AATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTT  >  NC_002947/2063053‑2063347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑