Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,063,208 G→A 16.7% intergenic (+65/‑142) PP_1838 → / → PP_1839 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,063,2080GA16.7% 132.3 / 15.5 59intergenic (+65/‑142)PP_1838/PP_1839membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/27);  new base A (3/6);  total (23/36)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGA  >  NC_002947/2063062‑2063353
                                                                                                                                                   |                                                                                                                                                   
gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGAc                                                                                                                                                   >  4:10975/1‑149 (MQ=255)
   gCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                                <  2:319805/149‑1 (MQ=255)
    cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCCTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                                <  1:247118/149‑1 (MQ=255)
    cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGACTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                                <  1:241600/149‑1 (MQ=255)
    cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTtg                                                                                                                                               <  7:142111/149‑1 (MQ=255)
     tGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTtgt                                                                                                                                              >  3:85425/1‑149 (MQ=255)
        taGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGGGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑c                                                                                                                                           <  6:314088/147‑1 (MQ=255)
         aGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAg                                                                                                                                         <  3:10975/149‑1 (MQ=255)
          gCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑Caat                                                                                                                                        >  6:359465/1‑147 (MQ=25)
                 tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCtgtg                                                                                                                                 <  4:345335/149‑1 (MQ=255)
                   cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGc                                                                                                                                <  3:174125/148‑1 (MQ=255)
                    aGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGa                                                                                                                              <  5:211340/149‑1 (MQ=18)
                     gACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAg                                                                                                                             <  5:359465/149‑1 (MQ=18)
                      aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGc                                                                                                                            >  1:274780/1‑149 (MQ=255)
                      aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGc                                                                                                                            <  5:384026/149‑1 (MQ=255)
                        cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCgg                                                                                                                          <  2:386717/149‑1 (MQ=18)
                        cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCgg                                                                                                                          <  2:274780/149‑1 (MQ=255)
                        cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCgg                                                                                                                          <  3:202096/149‑1 (MQ=255)
                        cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CGCCCACAGGCATTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCgg                                                                                                                          <  8:123269/149‑1 (MQ=25)
                          cgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCG‑CTGCCACAGGGATTGCACAGGGTTCGGACCATGTT‑CACGTACTGTTCGAGCagg                                                                                                                         >  4:116361/1‑148 (MQ=255)
                                  agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAACCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                                                <  5:71112/149‑1 (MQ=255)
                                   gCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                                                >  6:127688/1‑148 (MQ=255)
                                    ccTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCGTGTG‑CAGGTCCTGTGCGCGCGGGCCAGCCcgcg                                                                                                               >  5:6204/1‑148 (MQ=255)
                                       gctgGATACCACCGCCGGCGTGGGGCACGGCACACCGAATTACACCGGCGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGCCAGTGCACAGGGTTCGGCCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                           <  1:30098/149‑1 (MQ=37)
                                       gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTGCCACAGGCATTTCACAGGGTTCGGACCTTGTG‑CAGGTCCTTTGCGAGCGGGCAAGCCCGCGAATg                                                                                                           >  8:182584/1‑149 (MQ=255)
                                       gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGCAAACCGGGGCCGCGTGGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACCGCCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                           <  3:18942/149‑1 (MQ=255)
                                        ctgGATAACCCCGCCGGCGTGGTGCACGGAACACCGAAGTAACCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACCGGGTTCGGACCTTGTG‑CAGGTCCTGTGCTAGCGGGCAAGCCCGCGAATg                                                                                                           <  6:6204/148‑1 (MQ=38)
                                        ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCCCAGGCATTGGACAGGGGTGGGCCCTTGTG‑CAGGGCCGGTGCGAGCGGGCTAGCCCGCGAATg                                                                                                           >  2:30098/1‑148 (MQ=38)
                                        ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                           <  5:127688/148‑1 (MQ=255)
                                           gATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATGCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATgg                                                                                                          <  1:251588/147‑1 (MQ=255)
                                                cacCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGACCG‑CTCCCACAGGCATTGCACAGGGTTAGGACCTTGTG‑GAGGTCCTGTGCGAGCCGGCAAGCCCGCCAAAGGGCCCCGcc                                                                                                  >  6:380169/1‑148 (MQ=255)
                                                 acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCG‑CTCCCCCAGGGCTTGGACAGGGTTCGGGCCTTGTG‑GAGGGGCGGTGGGCGCGGGCCAGCCCGCGGATGGGGCCCGcc                                                                                                  >  1:239375/1‑147 (MQ=255)
                                                        gCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTc                                                                                          <  4:85425/149‑1 (MQ=255)
                                                               gCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCAAAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                                   >  2:107453/1‑149 (MQ=255)
                                                                cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTCCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTg                                                                                  <  5:380169/149‑1 (MQ=255)
                                                                cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CACCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                                   >  6:311707/1‑148 (MQ=255)
                                                                        acCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACACGCAGTGCACAGGGGTCGGCCCTTGTG‑CCGGGCCTGGGCGAGCGGGCACGCCCGCGAATGGGGCCCGCCCAAGTTTCCCGGCCGG‑ACCAGTAt                                                                         >  7:126387/1‑149 (MQ=25)
                                                                           gAAGTAACCGGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CGCCCACAGCCATTGCACCGGGTGCGCCCCTTGTG‑CAGGTCCTGGGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATccc                                                                      <  8:213814/149‑1 (MQ=37)
                                                                                      gggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGt                                                           <  5:311707/149‑1 (MQ=255)
                                                                                      gggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGt                                                           <  7:182584/149‑1 (MQ=255)
                                                                                        cccGCGTTGCGGCCCATTCGCGGGCGTGCCCG‑CTCCCACAGGCATGCCACAGGGTGCGCCCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATTCCCGCCCCAAGGTCa                                                         <  6:301386/148‑1 (MQ=38)
                                                                                        cccGCGTGGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGCACCTTGTC‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa                                                                                     <  7:120849/121‑1 (MQ=255)
                                                                                                        ttCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGCCTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcac                                         >  5:140953/1‑149 (MQ=14)
                                                                                                           gcgGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcacaca                                      >  1:354211/1‑149 (MQ=255)
                                                                                                                 ttGCCCG‑CTCCCACAGGCTTTGCACTGGGTGCGGACGTTGTG‑CAGGTCGTGTGCGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgt                                <  5:206649/149‑1 (MQ=255)
                                                                                                                   gCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt                              >  8:206854/1‑149 (MQ=255)
                                                                                                                     ccG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCa                                                 >  2:153087/1‑128 (MQ=14)
                                                                                                                     ccG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCa                                                 <  1:153087/128‑1 (MQ=14)
                                                                                                                       g‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                          <  2:239375/149‑1 (MQ=255)
                                                                                                                         cTCCCATAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGCAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                          <  1:107453/149‑1 (MQ=255)
                                                                                                                             cacaGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt                     >  8:107256/1‑149 (MQ=255)
                                                                                                                               caGGCCCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga                   <  6:140953/149‑1 (MQ=21)
                                                                                                                                aGGCATTGCACCGGGTTCGGACCTTGTG‑CTGGTCCTGTGCGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag                  <  2:354211/149‑1 (MQ=255)
                                                                                                                                aGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag                  >  5:43139/1‑149 (MQ=255)
                                                                                                                                      tGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTc            >  6:68140/1‑149 (MQ=255)
                                                                                                                                        cacaGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCCGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGTTCCg          >  6:215879/1‑149 (MQ=255)
                                                                                                                                         acaGGGTTCGGACCTTCTG‑CAGGTCCTGACCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAAATTTCCCGGACTG‑AACATTAACCCCGCCCCAAGGTCAGTTTTTCAGGCATCACACACCAGGTGTACGTTGACAGAGAGCGTCCGt         >  2:390978/1‑149 (MQ=255)
                                                                                                                                          ctgggTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATTGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt        <  3:116361/147‑1 (MQ=255)
                                                                                                                                                 cGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGa  <  7:107256/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGA  >  NC_002947/2063062‑2063353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑