Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,063,212 T→C 15.9% intergenic (+69/‑138) PP_1838 → / → PP_1839 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,063,2120TC15.9% 127.1 / 15.2 58intergenic (+69/‑138)PP_1838/PP_1839membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/29);  new base C (3/6);  total (23/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-01

GCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGT  >  NC_002947/2063065‑2063359
                                                                                                                                                    |                                                                                                                                                     
gCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                                      <  2:319805/149‑1 (MQ=255)
 cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCCTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                                      <  1:247118/149‑1 (MQ=255)
 cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGACTCCCACAGGCATTGCACAGGGTTCGGACCtt                                                                                                                                                      <  1:241600/149‑1 (MQ=255)
 cTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTtg                                                                                                                                                     <  7:142111/149‑1 (MQ=255)
  tGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTtgt                                                                                                                                                    >  3:85425/1‑149 (MQ=255)
     taGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGGGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑c                                                                                                                                                 <  6:314088/147‑1 (MQ=255)
      aGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAg                                                                                                                                               <  3:10975/149‑1 (MQ=255)
       gCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑Caat                                                                                                                                              >  6:359465/1‑147 (MQ=25)
              tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCtgtg                                                                                                                                       <  4:345335/149‑1 (MQ=255)
                cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGc                                                                                                                                      <  3:174125/148‑1 (MQ=255)
                 aGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGa                                                                                                                                    <  5:211340/149‑1 (MQ=18)
                  gACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAg                                                                                                                                   <  5:359465/149‑1 (MQ=18)
                   aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGc                                                                                                                                  <  5:384026/149‑1 (MQ=255)
                   aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGc                                                                                                                                  >  1:274780/1‑149 (MQ=255)
                     cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCgg                                                                                                                                <  2:386717/149‑1 (MQ=18)
                     cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCgg                                                                                                                                <  2:274780/149‑1 (MQ=255)
                     cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCgg                                                                                                                                <  3:202096/149‑1 (MQ=255)
                     cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CGCCCACAGGCATTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCgg                                                                                                                                <  8:123269/149‑1 (MQ=25)
                       cgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCG‑CTGCCACAGGGATTGCACAGGGTTCGGACCATGTT‑CACGTACTGTTCGAGCagg                                                                                                                               >  4:116361/1‑148 (MQ=255)
                               agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAACCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                                                      <  5:71112/149‑1 (MQ=255)
                                gCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                                                      >  6:127688/1‑148 (MQ=255)
                                 ccTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCGTGTG‑CAGGTCCTGTGCGCGCGGGCCAGCCcgcg                                                                                                                     >  5:6204/1‑148 (MQ=255)
                                    gctgGATACCACCGCCGGCGTGGGGCACGGCACACCGAATTACACCGGCGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGCCAGTGCACAGGGTTCGGCCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                                 <  1:30098/149‑1 (MQ=37)
                                    gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTGCCACAGGCATTTCACAGGGTTCGGACCTTGTG‑CAGGTCCTTTGCGAGCGGGCAAGCCCGCGAATg                                                                                                                 >  8:182584/1‑149 (MQ=255)
                                    gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGCAAACCGGGGCCGCGTGGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACCGCCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                                 <  3:18942/149‑1 (MQ=255)
                                     ctgGATAACCCCGCCGGCGTGGTGCACGGAACACCGAAGTAACCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACCGGGTTCGGACCTTGTG‑CAGGTCCTGTGCTAGCGGGCAAGCCCGCGAATg                                                                                                                 <  6:6204/148‑1 (MQ=38)
                                     ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCCCAGGCATTGGACAGGGGTGGGCCCTTGTG‑CAGGGCCGGTGCGAGCGGGCTAGCCCGCGAATg                                                                                                                 >  2:30098/1‑148 (MQ=38)
                                     ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                                 <  5:127688/148‑1 (MQ=255)
                                        gATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATGCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATgg                                                                                                                <  1:251588/147‑1 (MQ=255)
                                             cacCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGACCG‑CTCCCACAGGCATTGCACAGGGTTAGGACCTTGTG‑GAGGTCCTGTGCGAGCCGGCAAGCCCGCCAAAGGGCCCCGcc                                                                                                        >  6:380169/1‑148 (MQ=255)
                                              acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCG‑CTCCCCCAGGGCTTGGACAGGGTTCGGGCCTTGTG‑GAGGGGCGGTGGGCGCGGGCCAGCCCGCGGATGGGGCCCGcc                                                                                                        >  1:239375/1‑147 (MQ=255)
                                                     gCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTc                                                                                                <  4:85425/149‑1 (MQ=255)
                                                            gCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCAAAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                                         >  2:107453/1‑149 (MQ=255)
                                                             cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTCCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTg                                                                                        <  5:380169/149‑1 (MQ=255)
                                                             cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CACCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                                         >  6:311707/1‑148 (MQ=255)
                                                                     acCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACACGCAGTGCACAGGGGTCGGCCCTTGTG‑CCGGGCCTGGGCGAGCGGGCACGCCCGCGAATGGGGCCCGCCCAAGTTTCCCGGCCGG‑ACCAGTAt                                                                               >  7:126387/1‑149 (MQ=25)
                                                                        gAAGTAACCGGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CGCCCACAGCCATTGCACCGGGTGCGCCCCTTGTG‑CAGGTCCTGGGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATccc                                                                            <  8:213814/149‑1 (MQ=37)
                                                                                   gggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGt                                                                 <  5:311707/149‑1 (MQ=255)
                                                                                   gggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGt                                                                 <  7:182584/149‑1 (MQ=255)
                                                                                     cccGCGTTGCGGCCCATTCGCGGGCGTGCCCG‑CTCCCACAGGCATGCCACAGGGTGCGCCCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATTCCCGCCCCAAGGTCa                                                               <  6:301386/148‑1 (MQ=38)
                                                                                     cccGCGTGGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGCACCTTGTC‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa                                                                                           <  7:120849/121‑1 (MQ=255)
                                                                                                     ttCGCGGGCTTGCCCG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGCCTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcac                                               >  5:140953/1‑149 (MQ=14)
                                                                                                        gcgGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcacaca                                            >  1:354211/1‑149 (MQ=255)
                                                                                                              ttGCCCG‑CTCCCACAGGCTTTGCACTGGGTGCGGACGTTGTG‑CAGGTCGTGTGCGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgt                                      <  5:206649/149‑1 (MQ=255)
                                                                                                                gCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt                                    >  8:206854/1‑149 (MQ=255)
                                                                                                                  ccG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCa                                                       <  1:153087/128‑1 (MQ=14)
                                                                                                                  ccG‑CTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCa                                                       >  2:153087/1‑128 (MQ=14)
                                                                                                                    g‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                                <  2:239375/149‑1 (MQ=255)
                                                                                                                      cTCCCATAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGCAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                                <  1:107453/149‑1 (MQ=255)
                                                                                                                          cacaGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt                           >  8:107256/1‑149 (MQ=255)
                                                                                                                            caGGCCCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga                         <  6:140953/149‑1 (MQ=21)
                                                                                                                             aGGCATTGCACCGGGTTCGGACCTTGTG‑CTGGTCCTGTGCGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag                        <  2:354211/149‑1 (MQ=255)
                                                                                                                             aGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag                        >  5:43139/1‑149 (MQ=255)
                                                                                                                                   tGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTc                  >  6:68140/1‑149 (MQ=255)
                                                                                                                                     cacaGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCCGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGTTCCg                >  6:215879/1‑149 (MQ=255)
                                                                                                                                      acaGGGTTCGGACCTTCTG‑CAGGTCCTGACCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAAATTTCCCGGACTG‑AACATTAACCCCGCCCCAAGGTCAGTTTTTCAGGCATCACACACCAGGTGTACGTTGACAGAGAGCGTCCGt               >  2:390978/1‑149 (MQ=255)
                                                                                                                                       ctgggTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATTGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt              <  3:116361/147‑1 (MQ=255)
                                                                                                                                              cGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGa        <  7:107256/148‑1 (MQ=255)
                                                                                                                                               ggcccTTGTG‑CAGGGCCGGGGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGaaa      <  5:6482/146‑1 (MQ=255)
                                                                                                                                                 aCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAaga    <  7:206854/149‑1 (MQ=255)
                                                                                                                                                   cTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCAGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGt  >  3:285633/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCG‑CTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGT  >  NC_002947/2063065‑2063359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑