Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,063,220 G→T 13.7% intergenic (+77/‑130) PP_1838 → / → PP_1839 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,063,2200GT13.7% 135.6 / 11.0 55intergenic (+77/‑130)PP_1838/PP_1839membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/27);  new base T (2/6);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-01

TCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAA  >  NC_002947/2063079‑2063364
                                                                                                                                              |                                                                                                                                                 
tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCtgtg                                                                                                                                            <  4:345335/149‑1 (MQ=255)
  cAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGc                                                                                                                                           <  3:174125/148‑1 (MQ=255)
   aGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGa                                                                                                                                         <  5:211340/149‑1 (MQ=18)
    gACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAg                                                                                                                                        <  5:359465/149‑1 (MQ=18)
     aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGc                                                                                                                                       <  5:384026/149‑1 (MQ=255)
     aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGc                                                                                                                                       >  1:274780/1‑149 (MQ=255)
       cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCgg                                                                                                                                     <  2:386717/149‑1 (MQ=18)
       cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCgg                                                                                                                                     <  2:274780/149‑1 (MQ=255)
       cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCgg                                                                                                                                     <  3:202096/149‑1 (MQ=255)
       cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCGCCCACAGGCATTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCgg                                                                                                                                     <  8:123269/149‑1 (MQ=25)
         cgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCGCTGCCACAGGGATTGCACAGGGTTCGGACCATGTT‑CACGTACTGTTCGAGCagg                                                                                                                                    >  4:116361/1‑148 (MQ=255)
                 agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAACCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                                                           <  5:71112/149‑1 (MQ=255)
                  gCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                                                           >  6:127688/1‑148 (MQ=255)
                   ccTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCGTGTG‑CAGGTCCTGTGCGCGCGGGCCAGCCcgcg                                                                                                                          >  5:6204/1‑148 (MQ=255)
                      gctgGATACCACCGCCGGCGTGGGGCACGGCACACCGAATTACACCGGCGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGCCAGTGCACAGGGTTCGGCCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                                      <  1:30098/149‑1 (MQ=37)
                      gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTGCCACAGGCATTTCACAGGGTTCGGACCTTGTG‑CAGGTCCTTTGCGAGCGGGCAAGCCCGCGAATg                                                                                                                      >  8:182584/1‑149 (MQ=255)
                      gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGCAAACCGGGGCCGCGTGGCGGCCCATTCGCGGGCTTGCCCGCTCCCACCGCCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                                      <  3:18942/149‑1 (MQ=255)
                       ctgGATAACCCCGCCGGCGTGGTGCACGGAACACCGAAGTAACCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACCGGGTTCGGACCTTGTG‑CAGGTCCTGTGCTAGCGGGCAAGCCCGCGAATg                                                                                                                      <  6:6204/148‑1 (MQ=38)
                       ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGGACAGGGGTGGGCCCTTGTG‑CAGGGCCGGTGCGAGCGGGCTAGCCCGCGAATg                                                                                                                      >  2:30098/1‑148 (MQ=38)
                       ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                                      <  5:127688/148‑1 (MQ=255)
                          gATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATGCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATgg                                                                                                                     <  1:251588/147‑1 (MQ=255)
                               cacCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGACCGCTCCCACAGGCATTGCACAGGGTTAGGACCTTGTG‑GAGGTCCTGTGCGAGCCGGCAAGCCCGCCAAAGGGCCCCGcc                                                                                                             >  6:380169/1‑148 (MQ=255)
                                acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCGCTCCCCCAGGGCTTGGACAGGGTTCGGGCCTTGTG‑GAGGGGCGGTGGGCGCGGGCCAGCCCGCGGATGGGGCCCGcc                                                                                                             >  1:239375/1‑147 (MQ=255)
                                       gCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTc                                                                                                     <  4:85425/149‑1 (MQ=255)
                                              gCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCAAAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                                              >  2:107453/1‑149 (MQ=255)
                                               cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTCCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTg                                                                                             <  5:380169/149‑1 (MQ=255)
                                               cACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCACCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                                              >  6:311707/1‑148 (MQ=255)
                                                       acCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACACGCAGTGCACAGGGGTCGGCCCTTGTG‑CCGGGCCTGGGCGAGCGGGCACGCCCGCGAATGGGGCCCGCCCAAGTTTCCCGGCCGG‑ACCAGTAt                                                                                    >  7:126387/1‑149 (MQ=25)
                                                          gAAGTAACCGGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCGCCCACAGCCATTGCACCGGGTGCGCCCCTTGTG‑CAGGTCCTGGGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATccc                                                                                 <  8:213814/149‑1 (MQ=37)
                                                                     gggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGt                                                                      <  7:182584/149‑1 (MQ=255)
                                                                     gggCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGt                                                                      <  5:311707/149‑1 (MQ=255)
                                                                       cccGCGTTGCGGCCCATTCGCGGGCGTGCCCGCTCCCACAGGCATGCCACAGGGTGCGCCCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATTCCCGCCCCAAGGTCa                                                                    <  6:301386/148‑1 (MQ=38)
                                                                       cccGCGTGGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGCACCTTGTC‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa                                                                                                <  7:120849/121‑1 (MQ=255)
                                                                                       ttCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGCCTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcac                                                    >  5:140953/1‑149 (MQ=14)
                                                                                          gcgGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcacaca                                                 >  1:354211/1‑149 (MQ=255)
                                                                                                ttGCCCGCTCCCACAGGCTTTGCACTGGGTGCGGACGTTGTG‑CAGGTCGTGTGCGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgt                                           <  5:206649/149‑1 (MQ=255)
                                                                                                  gCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt                                         >  8:206854/1‑149 (MQ=255)
                                                                                                    ccGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCa                                                            <  1:153087/128‑1 (MQ=14)
                                                                                                    ccGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCa                                                            >  2:153087/1‑128 (MQ=14)
                                                                                                      gCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                                     <  2:239375/149‑1 (MQ=255)
                                                                                                       cTCCCATAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGCAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGt                                     <  1:107453/149‑1 (MQ=255)
                                                                                                           cacaGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt                                >  8:107256/1‑149 (MQ=255)
                                                                                                             caGGCCCTGCACAAGGTCCGAACCCTGTG‑CAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga                              <  6:140953/149‑1 (MQ=21)
                                                                                                              aGGCATTGCACCGGGTTCGGACCTTGTG‑CTGGTCCTGTGCGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag                             <  2:354211/149‑1 (MQ=255)
                                                                                                              aGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag                             >  5:43139/1‑149 (MQ=255)
                                                                                                                    tGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTc                       >  6:68140/1‑149 (MQ=255)
                                                                                                                      cacaGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCCGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGTTCCg                     >  6:215879/1‑149 (MQ=255)
                                                                                                                       acaGGGTTCGGACCTTCTG‑CAGGTCCTGACCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAAATTTCCCGGACTG‑AACATTAACCCCGCCCCAAGGTCAGTTTTTCAGGCATCACACACCAGGTGTACGTTGACAGAGAGCGTCCGt                    >  2:390978/1‑149 (MQ=255)
                                                                                                                        ctgggTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATTGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt                   <  3:116361/147‑1 (MQ=255)
                                                                                                                               cGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGa             <  7:107256/148‑1 (MQ=255)
                                                                                                                                ggcccTTGTG‑CAGGGCCGGGGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGaaa           <  5:6482/146‑1 (MQ=255)
                                                                                                                                  aCCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAaga         <  7:206854/149‑1 (MQ=255)
                                                                                                                                    cTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCAGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGt       >  3:285633/1‑149 (MQ=255)
                                                                                                                                         g‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTggg    >  7:23241/1‑147 (MQ=255)
                                                                                                                                           cAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGaa  <  3:342235/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
TCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTG‑CAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTG‑AACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGGGAA  >  NC_002947/2063079‑2063364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑