Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,158,980 C→A 6.9% intergenic (+149/‑39) acpP → / → fabF acyl carrier protein/beta‑ketoacyl‑ACP synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,158,9800CA 6.9% 223.7 / 6.1 72intergenic (+149/‑39)acpP/fabFacyl carrier protein/beta‑ketoacyl‑ACP synthase II
Reads supporting (aligned to +/- strand):  ref base C (28/39);  new base A (3/2);  total (31/41)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

GCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTCTGGCTGGCCGCAGTGGCAT  >  NC_002947/2158835‑2159122
                                                                                                                                                 |                                                                                                                                              
gCTACATTCGACGTTTCTTTTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCATCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAAt                                                                                                                                             <  7:153172/149‑1 (MQ=255)
gCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAAt                                                                                                                                             <  7:321923/149‑1 (MQ=255)
gCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAAt                                                                                                                                             <  5:333171/149‑1 (MQ=255)
 cTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATg                                                                                                                                            <  6:27879/149‑1 (MQ=255)
   aCAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATgg                                                                                                                                           >  7:75839/1‑148 (MQ=255)
   aCAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCAGGCGTGCGGTTTTTTCATTGCGAGAGCCTCGCATTTTTCACGCGGCTTCGTTAACCGCGCACCAAGAGCCTGTTGCAATTTCATTCCAGCACTTGAATGCAATgg                                                                                                                                           >  3:62846/1‑148 (MQ=255)
         gACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGTCATTGCATTCAAGTGCTGGAATGAAATGGCAacag                                                                                                                                     <  7:166992/148‑1 (MQ=38)
          aCGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGAc                                                                                                                                   >  1:216586/1‑149 (MQ=255)
           cGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGAct                                                                                                                                  <  5:119186/149‑1 (MQ=255)
                cTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGt                                                                                                                              <  2:91240/148‑1 (MQ=255)
                 ttGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGGGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGtta                                                                                                                            >  1:59926/1‑149 (MQ=255)
                   gTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCAATCCAGCACTTGAATGCAATGGCAAGAGACTCTGtt                                                                                                                             <  1:247367/146‑1 (MQ=255)
                   gTCGGGGAAAAACGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGttat                                                                                                                           <  8:367074/148‑1 (MQ=255)
                    tCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGttatta                                                                                                                         <  3:387464/149‑1 (MQ=255)
                    tCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGt                                                                                                                              <  1:356108/144‑1 (MQ=255)
                    tCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGt                                                                                                                              >  2:356108/1‑144 (MQ=255)
                      ggggAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGa                                                                                                                       <  7:39150/149‑1 (MQ=255)
                      ggggAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGa                                                                                                                       <  5:384575/149‑1 (MQ=255)
                       gggAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGa                                                                                                                       >  8:129480/1‑148 (MQ=255)
                       gggAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGa                                                                                                                       >  6:151098/1‑148 (MQ=255)
                        ggAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaaa                                                                                                                     <  5:151098/149‑1 (MQ=255)
                        ggAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaa                                                                                                                      >  4:37639/1‑148 (MQ=255)
                         gAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaaaa                                                                                                                    <  3:48407/149‑1 (MQ=255)
                         gAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaa                                                                                                                      >  6:227316/1‑147 (MQ=255)
                          aaaaCCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAg                                                                                                                   >  3:343020/1‑149 (MQ=255)
                              ccGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGgaga                                                                                                               <  3:37639/149‑1 (MQ=255)
                              ccGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGgaga                                                                                                               <  1:242299/149‑1 (MQ=255)
                              ccGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGgaga                                                                                                               <  8:250836/149‑1 (MQ=255)
                               cGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGagag                                                                                                              >  4:39317/1‑149 (MQ=255)
                                  aCTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTAc                                                                                                           >  5:54509/1‑149 (MQ=255)
                                   cTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACt                                                                                                          <  6:264897/149‑1 (MQ=255)
                                    tGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGt                                                                                                                              <  4:387834/128‑1 (MQ=255)
                                    tGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGt                                                                                                                              >  3:387834/1‑128 (MQ=255)
                                      ccTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACtgtg                                                                                                       >  7:321781/1‑149 (MQ=255)
                                      ccTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACtgt                                                                                                        <  5:136914/148‑1 (MQ=255)
                                        ttCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGTCTCTTGCCATTGCATTCAAGTGCTGGAATGAAATGGCAACAGGctcttggtg                                                                                                                           >  7:197850/1‑122 (MQ=18)
                                        ttCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGTCTCTTGCCATTGCATTCAAGTGCTGGAATGAAATGGCAACAGGctcttggtg                                                                                                                           <  8:197850/127‑6 (MQ=18)
                                            cTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGt                                                                                                 <  2:216586/149‑1 (MQ=255)
                                             tGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTcgcg                                                                                                  >  6:324960/1‑147 (MQ=255)
                                                       ttttttCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGcgtcgt                                                                                      >  2:26063/1‑149 (MQ=255)
                                                       ttttttCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGcgtcgt                                                                                      >  4:51625/1‑149 (MQ=255)
                                                           ttCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTGTTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGt                                                                                   <  8:104361/148‑1 (MQ=255)
                                                             cTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCa                                                                                 <  6:388018/148‑1 (MQ=255)
                                                               ttGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCg                                                                              >  3:90309/1‑149 (MQ=255)
                                                                tGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCAc                                                                                <  7:218860/146‑1 (MQ=255)
                                                                  cGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCggt                                                                            <  2:246899/148‑1 (MQ=255)
                                                                         cTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTATTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCggtatgggtat                                                                    <  1:111731/149‑1 (MQ=255)
                                                                                       gcggcTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTg                                                                                     <  1:128937/118‑1 (MQ=255)
                                                                                       gcggcTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTg                                                                                     >  2:128937/1‑118 (MQ=255)
                                                                                            ttCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCCCCGGTATGGGTATGCTGTCGCCACTGGGTAcc                                                 >  7:131885/1‑149 (MQ=255)
                                                                                               gTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGa                                               <  5:227316/148‑1 (MQ=255)
                                                                                               gTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGAt                                              <  6:54509/149‑1 (MQ=255)
                                                                                               gTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGAt                                              <  2:59926/149‑1 (MQ=255)
                                                                                               gTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGAt                                              <  4:343020/149‑1 (MQ=255)
                                                                                               gTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGAt                                              <  8:321781/149‑1 (MQ=255)
                                                                                                   aCCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTAc                                          >  3:12576/1‑149 (MQ=255)
                                                                                                            cAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCt                                 >  5:256417/1‑149 (MQ=255)
                                                                                                                    tGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAggg                          >  3:242208/1‑148 (MQ=255)
                                                                                                                     cttGCCATTGCATTCAAGTGCTGGAATGAAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCa                        >  7:162306/2‑149 (MQ=38)
                                                                                                                      ttGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCAt                       >  1:159878/1‑149 (MQ=255)
                                                                                                                         ccATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTct                    <  4:12576/149‑1 (MQ=255)
                                                                                                                         ccATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTct                    >  8:352834/1‑149 (MQ=255)
                                                                                                                         ccATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTct                    <  7:129480/149‑1 (MQ=255)
                                                                                                                          cATTGCATTCAAGTGCTGGAATGAAATGGCAACAGGCTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCCCCGGTATGGGTATGCTGTCGCCACTGGGTACCGATATACCGAGCACCTGGCAGGGCAGTctg                   >  4:118531/1‑149 (MQ=37)
                                                                                                                           aTTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACAGGTATGGGTATGCTGTCGCGACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTctgg                  >  5:336126/1‑149 (MQ=255)
                                                                                                                            tttCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTctggc                 <  3:51625/149‑1 (MQ=255)
                                                                                                                              tCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTctggct                <  1:26063/148‑1 (MQ=255)
                                                                                                                                          ttGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTCTGGCTGGCCGCAGTg      >  5:104734/1‑146 (MQ=255)
                                                                                                                                          ttGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTCTGGCTGGCCGCAGTg      <  6:104734/146‑1 (MQ=255)
                                                                                                                                          ttGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTCTGGCTGGCCGCAGTGGCa   >  4:99239/1‑149 (MQ=255)
                                                                                                                                            gattGCATTGGCAAGAGAGTGTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTAGTGGTCACCGGTATGGTTTTGCTGTCGCCACTGGGTAGCGATGTACCGAGCAACTGTCAGTGCATTCTGGCTGGCCGCAGTGGCAt  <  4:62846/145‑1 (MQ=255)
                                                                                                                                            gAATGCAATTGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTCTGGCTGGCCGCAGTGGCAt  <  8:75839/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCGCATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTGTTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTCGCGTAGACGCGTCGTGGTCACCGGTATGGGTATGCTGTCGCCACTGGGTACCGATGTACCGAGCACCTGGCAGGGCATTCTGGCTGGCCGCAGTGGCAT  >  NC_002947/2158835‑2159122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑