Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,240,376	A→T	8.2%	D202V (GAC→GTC)	gltX →	glutamate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,240,376	0	A	T	8.2%	176.1 / 4.1	61	D202V (GAC→GTC)	gltX	glutamate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (27/28);  new base T (3/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

TGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGAAA  >  NC_002947/2240229‑2240524                                                                                                                                                     |                                                                                                                                                     tGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTcgac                                                                                                                                                     <  1:349767/149‑1 (MQ=255)   aTCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTcgacga                                                                                                                                                   <  8:316829/149‑1 (MQ=255)     ccGCATGAAGGTGCCGAGCCAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCTAGATCCCGTGTGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGCAGacc                                                                                                                                                 >  2:148242/1‑149 (MQ=255)           gAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGa                                                                                                                                           >  1:388485/1‑149 (MQ=255)                    gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCa                                                                                                                                  <  6:304466/149‑1 (MQ=255)                    gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCa                                                                                                                                  <  3:148065/149‑1 (MQ=255)                    gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCa                                                                                                                                  <  7:18272/149‑1 (MQ=255)                                   cGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGtgct                                                                                                                        <  8:323830/144‑1 (MQ=255)                                   cGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTg                                                                                                                   <  2:252388/149‑1 (MQ=255)                                       gtgCCGGACATGCTTCGCGGCGATGTCGAGATCCCGTGGGACCGCATTGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTTGCCAACGTGGGCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGa                                                                                                               <  1:148242/149‑1 (MQ=255)                                       gtgCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgggg                                                                                                                <  4:82635/148‑1 (MQ=255)                                       gtgCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgggg                                                                                                                <  2:129380/148‑1 (MQ=255)                                       gtgCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgggg                                                                                                                >  3:82635/1‑148 (MQ=255)                                          ccGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAag                                                                                                             <  5:362014/148‑1 (MQ=255)                                                    tgcGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCa                                                                                                  >  1:345642/1‑149 (MQ=255)                                                             aTGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgg                                                                                                                  >  3:17162/1‑124 (MQ=255)                                                             aTGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgg                                                                                                                  <  4:17162/124‑1 (MQ=255)                                                               gTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGccc                                                                                         >  1:337882/1‑147 (MQ=255)                                                                 cGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACTCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGc                                                                                     <  1:76571/149‑1 (MQ=255)                                                                  gagaTCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCt                                                                                    >  8:217416/1‑149 (MQ=255)                                                                    gaTCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGa                                                                                  <  6:207502/149‑1 (MQ=255)                                                                    gaTCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGa                                                                                  <  3:10758/149‑1 (MQ=255)                                                                     aTCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGa                                                                                  <  3:312066/148‑1 (MQ=255)                                                                     aTCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGa                                                                                  >  4:312066/1‑148 (MQ=255)                                                                         cGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCaaa                                                                             <  2:345642/149‑1 (MQ=255)                                                                              gACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTa                                                                        >  8:277839/1‑149 (MQ=255)                                                                                 cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGa                                                                     <  6:339313/149‑1 (MQ=255)                                                                                  gCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAg                                                                    >  7:301287/1‑149 (MQ=255)                                                                                  gCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAg                                                                    >  7:368575/1‑149 (MQ=255)                                                                                          aTGCAGGTGCTGATGAAGAACGACGGCCTGCGCGCCGAACTACATGGCCAACTTGGTCGACGCCCACCTGATGGGCATCACCCACGTGATGCGGGGGGAAGCGTGGCTGCCTGCGGCGCCCAACCTGATCAAACTGAACGAGTAATTCg                                                             >  1:240357/1‑149 (MQ=255)                                                                                            gCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcgc                                                                                           <  7:71203/116‑1 (MQ=255)                                                                                            gCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcgc                                                                                           >  8:71203/1‑116 (MQ=255)                                                                                            gCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCt                                                          >  8:58108/1‑149 (MQ=255)                                                                                              aGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTgg                                                        <  7:217416/149‑1 (MQ=255)                                                                                              aGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTgg                                                        >  3:243726/1‑149 (MQ=255)                                                                                               ggTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTggg                                                       <  1:166894/149‑1 (MQ=255)                                                                                                gTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCaa                                                                                       <  6:183843/116‑1 (MQ=255)                                                                                                gTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCaa                                                                                       >  5:183843/1‑116 (MQ=255)                                                                                                 tGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGag                                                     <  2:337882/149‑1 (MQ=255)                                                                                                    ccaggaagtaCGTCGGCAGGC‑CGTCGTTCTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGagcag                                                  >  6:107810/5‑149 (MQ=255)                                                                                                      atgaAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGagcagc                                                 <  5:133160/148‑1 (MQ=255)                                                                                                        gaagaaCGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                                                       >  2:93266/1‑124 (MQ=255)                                                                                                        gaagaaCGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                                                       <  1:93266/124‑1 (MQ=255)                                                                                                        gaagaaCGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGccc                                               >  4:365553/1‑148 (MQ=255)                                                                                                         aagaaCGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCa                                              <  4:243726/148‑1 (MQ=255)                                                                                                           gaaCGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGc                                           >  7:116775/1‑149 (MQ=255)                                                                                                               gacgGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACTAGTACTTCAGCTGGGAGCAGCCCAAGCtgtg                                       <  7:58108/149‑1 (MQ=255)                                                                                                               gacgGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCtgtg                                       >  7:363595/1‑149 (MQ=255)                                                                                                                   gCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTAc                                   <  3:365553/149‑1 (MQ=255)                                                                                                                     cTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACAt                                 <  8:301287/149‑1 (MQ=255)                                                                                                                      tGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATg                                >  1:197489/1‑149 (MQ=255)                                                                                                                        c‑cGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGcc                              >  2:377546/1‑149 (MQ=255)                                                                                                                          cGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCg                             >  6:103179/1‑149 (MQ=255)                                                                                                                            aCGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCgct                           >  4:141896/1‑149 (MQ=255)                                                                                                                             cGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCgctg                          <  8:368575/149‑1 (MQ=255)                                                                                                                                       ccatcaggTGGTCGTCGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAAcc                >  2:198348/8‑149 (MQ=255)                                                                                                                                         atcaggTGGTCGTCGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCg              <  1:198348/144‑1 (MQ=255)                                                                                                                                          tcaggTGGTCGTCGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCAAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCgctgct                        <  6:90217/134‑1 (MQ=255)                                                                                                                                          tcaggTGGTCGTCGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCAAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCgctgct                        >  5:90217/5‑138 (MQ=255)                                                                                                                                           aaCGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGa            >  7:8143/1‑149 (MQ=255)                                                                                                                                             ggTGGTCGTCGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGAc           >  6:71206/2‑148 (MQ=255)                                                                                                                                                ggTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAAt       >  4:295794/1‑149 (MQ=255)                                                                                                                                                  tCGACGCCCACCTGATGGGCATCACCCACGTGCAGCGTGGGGAAGAGTGGCTGCCAGCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGAGCTACATGCCGCTGCTGCGCACCCCGGACACATc      >  4:40200/1‑148 (MQ=255)                                                                                                                                                   cgacgaCCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATTCCGCTGCTGCGCACCCCGGACAAAGCg     >  4:214583/1‑148 (MQ=255)                                                                                                                                                   cgacgaCCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGa    <  2:240357/149‑1 (MQ=255)                                                                                                                                                    gacgacCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGaa   <  3:295794/149‑1 (MQ=255)                                                                                                                                                    gacgacCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGa    >  4:333317/1‑148 (MQ=255)                                                                                                                                                     acgacCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGaaa  <  3:333317/149‑1 (MQ=255)                                                                                                                                                     acgacCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGaaa  <  8:363595/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGC‑CGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGAAA  >  NC_002947/2240229‑2240524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑