Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,242,046	T→C	5.5%	intergenic (+44/‑18)	PP_t28 → / → PP_t29	tRNA‑Glu/tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,242,046	0	T	C	5.5%	223.1 / 2.1	73	intergenic (+44/‑18)	PP_t28/PP_t29	tRNA‑Glu/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base T (36/33);  new base C (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

GCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTC  >  NC_002947/2241900‑2242193                                                                                                                                                    |                                                                                                                                                    gcgTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATtg                                                                                                                                                    <  1:2385/148‑1 (MQ=255)  cgTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATtgat                                                                                                                                                  <  1:334616/149‑1 (MQ=255)  cgTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATtgat                                                                                                                                                  <  2:323491/149‑1 (MQ=255)  cgTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATtgat                                                                                                                                                  <  3:266905/149‑1 (MQ=255)   gTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCCATTTACCTGCGTTTTGCAGGGATTAAAAAGGCTCGTCGATTATtgatg                                                                                                                                                 >  4:224012/1‑149 (MQ=255)   gTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATtgat                                                                                                                                                  >  6:70801/1‑148 (MQ=255)    ttAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGattatt                                                                                                                                                     >  4:310705/1‑144 (MQ=255)    ttAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGattatt                                                                                                                                                     <  3:310705/144‑1 (MQ=255)        cGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCATCAATAATCGACGAGCCt                                                                                                                                            <  5:177248/149‑1 (MQ=18)            tCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTg                                                                                                                                        >  2:70040/1‑149 (MQ=255)            tCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTg                                                                                                                                        >  8:106299/1‑149 (MQ=255)            tCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGGCGATTATTGATGAGCCTTTTg                                                                                                                                        >  6:35918/1‑149 (MQ=255)                cACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTc                                                                                                                                    >  7:308039/1‑149 (MQ=255)                 aCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCg                                                                                                                                   >  5:17651/1‑149 (MQ=255)                 aCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCg                                                                                                                                   >  4:259979/1‑149 (MQ=255)                 aCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCg                                                                                                                                   <  7:169181/149‑1 (MQ=255)                   gAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCggg                                                                                                                                 <  7:106299/149‑1 (MQ=255)                       gTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCATCAATAATCGACGAGCCt                                                                                                                                            >  4:201296/1‑134 (MQ=17)                       gTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCATCAATAATCGACGAGCCt                                                                                                                                            <  3:201296/134‑1 (MQ=17)                            gTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCt                                                                                                                        <  4:99179/149‑1 (MQ=255)                            gTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCt                                                                                                                        >  6:141912/1‑149 (MQ=255)                            gTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCt                                                                                                                        <  7:53859/149‑1 (MQ=255)                              ccccTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCa                                                                                                                      >  2:336144/1‑149 (MQ=255)                                ccTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCtata                                                                                                                           >  1:258284/1‑142 (MQ=255)                                ccTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCtata                                                                                                                           <  2:258284/142‑1 (MQ=255)                                 cTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCt                                                                                                                   <  7:58172/149‑1 (MQ=255)                                          gTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGc                                                                                                                               <  4:227484/128‑1 (MQ=255)                                          gTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGc                                                                                                                               >  3:227484/1‑128 (MQ=255)                                          gTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGgaga                                                                                                             <  4:68049/146‑1 (MQ=255)                                          gTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGaaga                                                                                                             <  5:20847/146‑1 (MQ=255)                                                  ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatg                                                                                                  <  6:365557/149‑1 (MQ=255)                                                  ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcat                                                                                                   <  6:17651/148‑1 (MQ=255)                                                  ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcat                                                                                                   <  5:77359/148‑1 (MQ=255)                                                     cacCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggca                                                                                               <  3:259979/149‑1 (MQ=255)                                                      acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGACTTTTGTTTAGGGGCTATAGCTAAGCTGGGAGAGAGCCTgcaaggcat                                                                                              >  7:59073/1‑149 (MQ=37)                                                        cGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATtgatga                                                                                                                                                >  2:90373/1‑97 (MQ=255)                                                        cGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATtgatga                                                                                                                                                <  1:90373/97‑1 (MQ=255)                                                        cGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcatg                                                                                             >  4:127910/1‑148 (MQ=255)                                                        cGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcatg                                                                                             >  1:143213/1‑148 (MQ=255)                                                        cGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGc                                                                                            <  2:143213/149‑1 (MQ=255)                                                         tcccTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCa                                                                                           <  3:13904/148‑1 (MQ=255)                                                           ccTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCa                                                                                           >  4:13904/1‑147 (MQ=255)                                                             tttCACGGCGGTAACAGGGGTTCGAGTCGCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatg                                                                                                  >  1:221763/1‑138 (MQ=255)                                                             tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatg                                                                                                  <  2:221763/138‑1 (MQ=255)                                                             tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCaggag                                                                                       >  2:334922/1‑149 (MQ=255)                                                               tCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCaggag                                                                                       <  5:70801/147‑1 (MQ=255)                                                               tCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCaggag                                                                                       <  1:334922/147‑1 (MQ=255)                                                               tCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCaggag                                                                                       <  3:224012/147‑1 (MQ=255)                                                                 aCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCt                                                                                                       >  1:262010/1‑130 (MQ=255)                                                                 aCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCt                                                                                                       <  2:262010/129‑1 (MQ=255)                                                                 aCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGt                                                                                     >  5:181273/1‑147 (MQ=255)                                                                            aGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATc                                                                        <  5:35918/149‑1 (MQ=255)                                                                            aGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCGCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATc                                                                        <  7:270328/149‑1 (MQ=255)                                                                                gTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGc                                                                    >  2:24863/1‑149 (MQ=255)                                                                                      gTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCt                                                              >  7:56162/1‑149 (MQ=255)                                                                                       tCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGGCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTc                                                             >  6:180975/1‑149 (MQ=255)                                                                                         cccTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTcca                                                           <  1:24863/149‑1 (MQ=255)                                                                                          ccTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac                                                          >  3:230813/1‑149 (MQ=255)                                                                                            tAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccacca                                                        >  1:188987/1‑149 (MQ=255)                                                                                                 gAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCt                                                   <  4:230813/149‑1 (MQ=255)                                                                                                      ccACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGc                                                 >  2:113259/1‑147 (MQ=255)                                                                                                       cACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAg                                              >  1:326563/1‑149 (MQ=255)                                                                                                             tCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGa                                                                          <  8:275863/115‑1 (MQ=255)                                                                                                             tCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGa                                                                          >  7:275863/1‑115 (MQ=255)                                                                                                                tGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTGGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACg                                      >  6:238120/1‑148 (MQ=255)                                                                                                                    ttttGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGt                                 <  1:336144/149‑1 (MQ=255)                                                                                                                    ttttGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGt                                 <  6:181273/149‑1 (MQ=255)                                                                                                                        gCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGAt                             >  1:343033/1‑149 (MQ=255)                                                                                                                          aGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCt                           <  2:188987/149‑1 (MQ=255)                                                                                                                           gggCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCtt                          >  3:117992/1‑149 (MQ=255)                                                                                                                           gggCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCtt                          >  5:11075/1‑149 (MQ=255)                                                                                                                            ggCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATc                                                                        >  4:211536/1‑102 (MQ=255)                                                                                                                            ggCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATc                                                                        <  3:211536/102‑1 (MQ=255)                                                                                                                            ggCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTc                         <  2:326563/149‑1 (MQ=255)                                                                                                                              cTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTcgc                       <  5:141912/149‑1 (MQ=255)                                                                                                                                  aaaaGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTTCACCTTTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg                   <  1:70040/149‑1 (MQ=255)                                                                                                                                  aaaaGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg                   >  4:340196/1‑149 (MQ=255)                                                                                                                                     tggCTCGTCTTTTATTTATGATCCTTTTTTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCAAGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGTTTTACCATTTCTGTCAGGGTTCATGTAAGTGGATCTTCGCGAAGGtt                <  8:59073/148‑1 (MQ=18)                                                                                                                                       gCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGAATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTAc              >  5:128086/1‑149 (MQ=255)                                                                                                                                       gCTCATCAATAATCGACGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTAc              >  8:92233/1‑149 (MQ=21)                                                                                                                                           gtcgATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTcc          >  8:271567/1‑149 (MQ=255)                                                                                                                                              gATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCt       >  6:45549/1‑149 (MQ=255)                                                                                                                                              gATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCt       >  3:48047/1‑149 (MQ=255)                                                                                                                                                   ttGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTc  >  5:77447/1‑149 (MQ=255)                                                                                                                                                   ttGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTc  <  3:340196/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAC‑GCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTC  >  NC_002947/2241900‑2242193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑