Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,247,813 T→C 7.3% S596G (AGC→GGC) ‡ PP_1983 ← PAS/PAC/GAF sensor‑containing diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,247,8130TC 7.3% 189.5 / 5.1 68S596G (AGC→GGC) ‡PP_1983PAS/PAC/GAF sensor‑containing diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (28/35);  new base C (3/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

GGGCGGTCAGCAGCAGGCAATCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCGGTGATGGTGATGGCGT  >  NC_002947/2247666‑2247956
                                                                                                                                                     |                                                                                                                                                
gggcggTCAGCAGCAGGCAATCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCgcgctg                                                                                                                                                 >  3:236640/1‑149 (MQ=255)
     gTCAGCAGCAGGCAATCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGcgctgcgctg                                                                                                                                            <  3:315636/149‑1 (MQ=255)
      tCAGCAGCAGGCAATCCACTTCGTTGAGTGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATCTCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCgctgcgctgc                                                                                                                                           <  7:75756/149‑1 (MQ=255)
      tCAGCAGCAGGCAATCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCgctgcgctgc                                                                                                                                           >  1:65522/1‑149 (MQ=255)
        agcagcagGCAATCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCtt                                                                                                                                         <  4:351288/149‑1 (MQ=255)
        agcagcagGCAATCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCAGCGCgctgctg                                                                                                                                         <  7:34054/149‑2 (MQ=255)
                gCAATCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCgg                                                                                                                                 <  2:254208/149‑1 (MQ=255)
                    tCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTgt                                                                                                                                       <  6:238973/139‑1 (MQ=255)
                    tCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTgt                                                                                                                                       >  5:238973/1‑139 (MQ=255)
                    tCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGcc                                                                                                                               >  4:342836/1‑147 (MQ=255)
                       aCTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGAt                                                                                                                          <  8:48169/149‑1 (MQ=255)
                        cTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATg                                                                                                                         >  5:224466/1‑149 (MQ=255)
                        cTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGAGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATg                                                                                                                         >  2:289758/1‑149 (MQ=255)
                           cGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑c                                                                                                                       <  1:163017/147‑1 (MQ=255)
                            gTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGa                                                                                                                    >  1:254867/1‑149 (MQ=255)
                             ttGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGAt                                                                                                                   <  5:187992/149‑1 (MQ=255)
                                aGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGAtctc                                                                                                                <  4:352198/149‑1 (MQ=255)
                                aGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGAtct                                                                                                                 >  3:387810/1‑148 (MQ=255)
                                 gggTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTTCACAGGAAAGCTCGCTCAGACAAGGCCAGCAGCCCCGTGCGGTGGTTGTAGTCGGCCCCGATG‑CCGAtct                                                                                                                 >  1:126975/1‑148 (MQ=255)
                                    tGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATa                                                                                                             >  7:278531/1‑148 (MQ=255)
                                         ggTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCgt                                                                                                       <  6:224466/149‑1 (MQ=255)
                                          gTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCAGCGCgctgctggccga                                                                                                                                    <  6:235433/120‑7 (MQ=255)
                                          gTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCAGCGCgctgctggccga                                                                                                                                    >  5:235433/1‑114 (MQ=255)
                                              cACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGc                                                                                                  <  2:254867/149‑1 (MQ=255)
                                               aCCGAAACCTCGACCGTGAGGATATCGCCACGCTTGTTTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑AATCGGCCAGCAGCGCGATGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGc                                                                                                  >  1:64020/1‑149 (MQ=255)
                                                 cGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGa                                                                                               >  2:313584/1‑149 (MQ=255)
                                                  gAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGAt                                                                                              <  4:389437/149‑1 (MQ=255)
                                                   aaaCCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATc                                                                                             <  3:342836/149‑1 (MQ=255)
                                                      ccTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACt                                                                                          <  4:387810/149‑1 (MQ=255)
                                                        tCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACtt                                                                                         >  7:106205/1‑148 (MQ=255)
                                                          gACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTtc                                                                                        <  2:65522/147‑1 (MQ=255)
                                                                gAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTgg                                                                                                   <  7:362764/130‑1 (MQ=255)
                                                                gAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTgg                                                                                                   >  8:362764/1‑130 (MQ=255)
                                                                gAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGc                                                                                >  2:336485/1‑149 (MQ=255)
                                                                   gATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGt                                                                             <  7:277635/149‑1 (MQ=255)
                                                                    atatCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTa                                                                            <  5:143953/149‑1 (MQ=255)
                                                                       tCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGcc                                                                         <  8:278531/149‑1 (MQ=255)
                                                                       tCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGcc                                                                         <  8:106205/149‑1 (MQ=255)
                                                                          cctcGCTTG‑TTGCGCCCGAGGATCTGGGGGTTGTGTACCCGGCCGGGCTCGCGCA‑ACTCGGCCAGCATGGCGGTGCGCTGCTTGTGGTTGGCCCAGATGCCCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCgg                                                                       <  1:289758/146‑1 (MQ=255)
                                                                          ccACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGTCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGt                                                                      <  2:281282/149‑1 (MQ=255)
                                                                          ccACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGt                                                                      <  6:302722/149‑1 (MQ=255)
                                                                          ccACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGt                                                                      >  7:341689/1‑149 (MQ=255)
                                                                                ttg‑ttgCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAggcg                                                                >  8:69199/1‑149 (MQ=255)
                                                                                    ttgCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACTCCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAggcgg                                                               >  7:180616/1‑147 (MQ=255)
                                                                                      gcgcCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGCTG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAg                                                            >  3:134111/1‑148 (MQ=255)
                                                                                       cgcCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGaa                                                          <  1:313584/149‑1 (MQ=255)
                                                                                        gcCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAg                                                         >  8:19339/1‑149 (MQ=255)
                                                                                          ccGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAggcggc                                                              >  8:368823/1‑142 (MQ=255)
                                                                                          ccGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAggcggc                                                              <  7:368823/142‑1 (MQ=255)
                                                                                                aTCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGtt                                                 <  6:105411/149‑1 (MQ=255)
                                                                                                 tctcGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGtt                                                 >  8:116672/1‑148 (MQ=255)
                                                                                                   tcGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGAc                                              >  4:334075/1‑149 (MQ=255)
                                                                                                        gtggtgTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCa                                         <  3:334075/149‑1 (MQ=255)
                                                                                                           gtgtACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAgg                                       <  7:19339/148‑1 (MQ=255)
                                                                                                            tgtACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTa                                     <  4:236640/149‑1 (MQ=255)
                                                                                                               aCCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCTGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCg                                  >  8:76335/1‑149 (MQ=255)
                                                                                                               aCCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCg                                  >  7:6145/1‑149 (MQ=255)
                                                                                                                      cgcgCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCtg                           >  3:152095/1‑149 (MQ=255)
                                                                                                                       gcgcTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCtgt                          <  8:334019/149‑1 (MQ=255)
                                                                                                                             gcgcA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGtt                    <  6:213828/149‑1 (MQ=255)
                                                                                                                               gcA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCg                  >  4:169392/1‑149 (MQ=255)
                                                                                                                                 a‑aCTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggt                >  8:26171/1‑149 (MQ=255)
                                                                                                                                   aCTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggtg               <  7:168078/149‑1 (MQ=255)
                                                                                                                                     tCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggtgat             >  2:245613/1‑149 (MQ=255)
                                                                                                                                     tCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGGTCggtgat             >  4:93904/1‑149 (MQ=255)
                                                                                                                                       ggCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggtgatg            <  8:6145/148‑1 (MQ=255)
                                                                                                                                       ggCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggtgat             <  7:69199/147‑1 (MQ=255)
                                                                                                                                         caagcagcGCAGCGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggtgatggt          >  3:64055/3‑148 (MQ=255)
                                                                                                                                          cagcagCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggtgatggtga        <  4:134111/149‑1 (MQ=255)
                                                                                                                                          aagcagcGCAGCGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGTGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggtgatggtga        >  4:319387/2‑149 (MQ=255)
                                                                                                                                            gcagcGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCggtgatggtgat       <  1:336485/148‑1 (MQ=255)
                                                                                                                                                cgcgcTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCGGTGATGGTGATGGCGt  <  7:76335/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GGGCGGTCAGCAGCAGGCAATCCACTTCGTTGAGGGTGATCGGTTCCACCGAAACCTCGACCGTGAGGATATCGCCACGCTTG‑TTGCGCCCGAGCATCTCGCGGTGGTGTACCCGGCCGCGCTCGCGCA‑ACTCGGCCAGCAGCGCGCTGCGCTGCTTGTCGTCGGCCCAGATG‑CCGATCTCATACACCGTGTGGCCGATCACTTCATCAGTGCTGTAGCCGGTCAGGCGGCAGAAGCCGTCGTTGACCTCCAGGTAGCGGCCGCTGTGGCGTTCGGTGATGGTGATGGCGT  >  NC_002947/2247666‑2247956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑