Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,318,361 G→C 7.0% R201S (AGG→AGC)  PP_2039 → acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,318,3610GC 7.0% 178.9 / 4.2 57R201S (AGG→AGC) PP_2039acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (24/29);  new base C (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

AACACTATGTGCTGAACGGCGCCAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGTTTCAAGCTGGCGATGGGGCGTATCACCCTCAA  >  NC_002947/2318216‑2318501
                                                                                                                                                 |                                                                                                                                            
aaCACTATGTGCTGAACGGCGCCAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCC‑GGGGTAAGGGtt                                                                                                                                           <  4:75729/148‑1 (MQ=255)
aaCACTATGTGCTGAACGGCGCCAAGCCTTACATTTCGGGTGCGCCCAATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGCGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGt                                                                                                                                            <  2:176634/148‑1 (MQ=255)
     tATGTGCTGAACGGCGCCAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAg                                                                                                                                      >  1:100906/1‑149 (MQ=255)
         tgCTGAACGGCGCCAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGa                                                                                                                                   <  2:249796/148‑1 (MQ=255)
         tgCTGAACGGCGCCAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGa                                                                                                                                   <  3:294291/148‑1 (MQ=255)
         tgCTGAACGGCCCCAAGCGTTACCTTTCGTGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGa                                                                                                                                   <  6:209483/148‑1 (MQ=255)
             gAACGGCGCCAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGattatt                                                                                                                              >  3:344472/1‑149 (MQ=255)
             gAACGGCGCCAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGattat                                                                                                                               >  1:332681/1‑148 (MQ=255)
                      cAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCAGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGt                                                                                                                     >  3:18462/1‑149 (MQ=255)
                         gCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGAAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGGTTATTCGGTCATGTCcg                                                                                                                  <  2:383216/149‑1 (MQ=255)
                          cGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCtgg                                                                                                                 <  2:332681/149‑1 (MQ=255)
                          cGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCcgg                                                                                                                 <  4:344472/149‑1 (MQ=255)
                            ttACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCcggcg                                                                                                               >  7:86134/1‑149 (MQ=255)
                            ttACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTCCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCcggcg                                                                                                               <  7:112590/149‑1 (MQ=255)
                             tACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAg                                                                                                                                      <  4:329531/125‑1 (MQ=255)
                             tACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAg                                                                                                                                      >  3:329531/1‑125 (MQ=255)
                             tACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCACAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCcggcgg                                                                                                              >  3:283311/1‑149 (MQ=255)
                              aCATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCg                                                                                                                            >  7:41426/1‑134 (MQ=255)
                              aCATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCg                                                                                                                            <  8:41426/134‑1 (MQ=255)
                               cATGTCGGGTGCGCCCTAGGCGGACATTGCGTGGGTGCTTGCAGGGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGGATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTGCGGCggtg                                                                                                            <  8:321022/149‑1 (MQ=255)
                                aTTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGCGTTGAAAGTGATTATTCGGTCATGTCCGGCggtgg                                                                                                           >  7:303406/1‑149 (MQ=255)
                                aTTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGCGTTGAAAGTGATTATTCGGTCATGTCCGGCggtgg                                                                                                           >  7:297919/1‑149 (MQ=255)
                                 tttCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCggtggt                                                                                                          >  6:360377/1‑149 (MQ=255)
                                           gcCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGc                                                                                                 >  5:112336/1‑148 (MQ=255)
                                             catATGCCGACAGTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGAc                                                                                              <  8:64789/147‑1 (MQ=255)
                                                     gACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCt                                                                                      <  4:247458/149‑1 (MQ=255)
                                                     gACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCt                                                                                      <  4:334044/149‑1 (MQ=255)
                                                     gACATTGCGGTGCTGCTTGCAGTGACGGATCCTCGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCt                                                                                      <  4:252421/149‑1 (MQ=255)
                                                        aTTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTtga                                                                                   <  4:18462/149‑1 (MQ=255)
                                                         ttGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTtgat                                                                                  <  8:86134/149‑1 (MQ=255)
                                                               tgctgcTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTGGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGtt                                                                            <  1:178497/149‑1 (MQ=255)
                                                                   gctTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCggg                                                                        >  4:370123/1‑149 (MQ=255)
                                                                    ctTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGCGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCgg                                                                         <  8:303406/147‑1 (MQ=255)
                                                                    ctTGCAGAGACGGATCCTTGACGCGGTTCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGCGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCgg                                                                         <  8:297919/147‑1 (MQ=255)
                                                                            gACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCcggcgg                                                               <  4:195275/149‑1 (MQ=255)
                                                                             aCGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCggcggc                                                              >  2:143894/1‑149 (MQ=255)
                                                                               ggATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGcc                                                             >  4:165312/1‑148 (MQ=255)
                                                                               ggATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGcc                                                             >  2:48778/1‑148 (MQ=255)
                                                                                  tCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATc                                                         >  7:20232/1‑149 (MQ=255)
                                                                                      ggactgggTGCCCAAGGGATTTCGGCATTCTTGGTCGACCTGAGCGCGCCGTGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGTTGGTGGGGATGGCGACATCATGCTTGATGAc                                                                               <  8:281749/117‑1 (MQ=255)
                                                                                      ggACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGAc                                                                               >  7:281749/1‑123 (MQ=255)
                                                                                         aagaatGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTgg                                                  >  1:244209/6‑149 (MQ=255)
                                                                                                 ccAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAgcagg                                          <  8:20232/149‑1 (MQ=255)
                                                                                                  gaaGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAgcaggg                                         <  7:49080/148‑1 (MQ=255)
                                                                                                  cAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAgcaggg                                         >  5:102724/1‑149 (MQ=255)
                                                                                                   aaGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACg                                                                              <  6:240504/111‑1 (MQ=255)
                                                                                                   aaGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACg                                                                              >  5:240504/1‑111 (MQ=255)
                                                                                                       gATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAgcaggggcagg                                    <  3:370123/149‑1 (MQ=255)
                                                                                                        aTTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAgcaggggcaggg                                   >  4:165479/1‑149 (MQ=255)
                                                                                                        aTTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGAGGTGGTGCGCATGGCGACATGATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAgcaggggcaggg                                   >  8:195538/1‑149 (MQ=255)
                                                                                                            cGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGttt                                >  1:168380/1‑148 (MQ=255)
                                                                                                             ggCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGTTTc                               <  2:100906/148‑1 (MQ=255)
                                                                                                                   cttcGTCGACCTGAGCGCGCCGGTGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGTTTCAAGCTg                         <  5:231239/148‑1 (MQ=255)
                                                                                                                    ttcGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGTTTCAAGCTGGc                       >  4:380849/1‑149 (MQ=255)
                                                                                                                                         ggggTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGTTTCAAGCTGGCGATGGGGc               >  8:319243/1‑136 (MQ=255)
                                                                                                                                         ggggTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGTTTCAAGCTGGCGATGGGGc               <  7:319243/136‑1 (MQ=255)
                                                                                                                                         ggggTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGTTTCAAGCTGGCGATGGGGCGTATCACCCTCaa  <  2:168380/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
AACACTATGTGCTGAACGGCGCCAAGCGTTACATTTCGGGTGCGCCCTATGCCGACATTGCGGTGCTGCTTGCAGTGACGGATCCTGGACGCGGTGCCCAAGGGATTTCGGCATTCTTCGTCGACCTGAGCGCGCCGGGGGTAAGGGTTGAAAGTGATTATTCGGTCATGTCCGGCGGTGGTGCGCATGGCGACATCATGCTTGATGACGTTCGGGTGCCGGCGGCCAATCGCATTGGTGAGCAGGGGCAGGGTTTCAAGCTGGCGATGGGGCGTATCACCCTCAA  >  NC_002947/2318216‑2318501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑