Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,369,768 G→C 6.2% L862L (CTG→CTC) ‡ gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,369,7680GC 6.2% 204.6 / 4.3 65L862L (CTG→CTC) ‡gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base C (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

TTGCCCGTGGTGGCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATGACGACCCG  >  NC_002947/2369623‑2369912
                                                                                                                                                  |                                                                                                                                                
ttGCCCGTGGTGGCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGggc                                                                                                                                               >  7:170009/1‑149 (MQ=255)
  gCCCGTGGTGGCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGggcg                                                                                                                                              <  3:346146/148‑1 (MQ=255)
  gCCCGTGGTGGCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGggcg                                                                                                                                              <  2:129042/148‑1 (MQ=255)
    ccGTGGTGGCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGggcgg                                                                                                                                             >  3:120979/1‑147 (MQ=255)
      gtggtgGCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGc                                                                                                                                         >  7:256439/1‑149 (MQ=255)
         gtggCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGt                                                                                                                                      <  2:36216/149‑1 (MQ=255)
         gtggACTGCGCTGGTCCGACCTCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGATGGTGAAAGCCCAGAATGTGAAGAACTCGGTCATATTGCCTGTCTGCGCCAAGTGTTGTTTCATGCCGCGTCGCCTGCCGCCCAGCGGCAGGCga                                                                                                                                      <  2:76526/149‑2 (MQ=255)
           ggCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGa                                                                                                                                    <  5:2493/149‑1 (MQ=255)
            gCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGa                                                                                                                                    <  6:266026/148‑1 (MQ=255)
              cTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGt                                                                                                                                      <  4:230151/144‑1 (MQ=255)
              cTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGagat                                                                                                                                 >  3:230151/1‑146 (MQ=255)
                 cgcTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACgaga                                                                                                                               <  1:379103/148‑1 (MQ=255)
                  gcTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATc                                                                                                                             <  6:66410/149‑1 (MQ=255)
                         cGACCGCGAAGAGGACTTCCGTACCGAAGTGCTGGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACgag                                                                                                                                >  1:69807/1‑140 (MQ=255)
                         cGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACgag                                                                                                                                <  2:69807/139‑1 (MQ=255)
                          gACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGt                                                                                                                                      >  2:152075/1‑132 (MQ=255)
                          gACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGt                                                                                                                                      <  1:152075/132‑1 (MQ=255)
                          gACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGccgccg                                                                                                                      >  2:182997/1‑148 (MQ=255)
                             cgcgAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAgg                                                                                                                  >  5:370848/1‑149 (MQ=255)
                             cgcgAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAgg                                                                                                                  >  5:141582/1‑149 (MQ=255)
                              gcgAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAggc                                                                                                                 <  1:232394/149‑1 (MQ=255)
                              gcgAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAggc                                                                                                                 <  3:98554/149‑1 (MQ=255)
                                       aCTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGc                                                                                                        <  5:149092/149‑1 (MQ=255)
                                         ttCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTa                                                                                                      >  6:172544/1‑149 (MQ=255)
                                                ccGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATc                                                                                               <  4:120979/149‑1 (MQ=255)
                                                ccGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCACCGCCGAAGGCGTGGCGTGCTACCGCATc                                                                                               <  8:202125/149‑1 (MQ=255)
                                                 cGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCt                                                                                              >  8:320304/1‑149 (MQ=255)
                                                 cGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCt                                                                                              >  5:223247/1‑149 (MQ=255)
                                                 cGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCt                                                                                              >  5:41915/1‑149 (MQ=255)
                                                         t‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTcc                                                                                      >  1:53271/1‑149 (MQ=255)
                                                               cTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCt                                                                                 >  4:100474/1‑149 (MQ=255)
                                                                   tGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTc                                                                             <  8:170009/149‑1 (MQ=255)
                                                                          ccAGCAGGTTAAGAACTCGGTCATCGTTCCGGTCGGAGCCAAGGGTGGTTTCCTGCCGCTTAGCCTGCCGCTTGGCGGCAGCCGTTAAGAGATCGCCGCCGAAGGAGTTGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCa                                                                      <  1:61108/149‑1 (MQ=255)
                                                                              cagGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGa                                                                  <  8:256439/149‑1 (MQ=255)
                                                                                ggTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCAc                                                                     >  4:157680/1‑144 (MQ=255)
                                                                                ggTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCAc                                                                     <  3:157680/144‑1 (MQ=255)
                                                                                             gTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTCCCGCATCTTCATTTCCGGCCTGCTCGACATCAGCGACAACCTCAAGGACgg                                                   >  8:68525/1‑149 (MQ=255)
                                                                                                atcggtccGGTCGGCGCCTTGGGTGGTTTCGTGCCGCTTCTCCTGCCGCTGGGCGGCAGTCGTGACGAGATCGCCGCCGATGGCGTGGCTTGCTACCTCATCTTCATTTCCGGCCTGCTCGACTTCAGCTAGAACCTCAAGTACGGTg                                                 <  2:170202/142‑1 (MQ=255)
                                                                                                              cgcCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCgg                                  <  1:182997/149‑1 (MQ=255)
                                                                                                              cgcCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCgg                                  <  2:53271/149‑1 (MQ=255)
                                                                                                               gcCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGc                                 >  3:278234/1‑149 (MQ=255)
                                                                                                                ccAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGAACGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGc                                 >  1:147413/1‑148 (MQ=255)
                                                                                                                 cAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGcc                                >  1:128805/1‑148 (MQ=255)
                                                                                                                   aGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATAGCCGGCGAAGGCGTGGCGTGCTACCGGATCGTCATTTCCGGCCTGCTCGACATCACCGACAACCGCAAGGACGGTGGCGTGGTGCCGCCGGGCAAc                             >  4:65764/1‑149 (MQ=255)
                                                                                                                       tggtTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCTCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACgtgg                         <  5:374391/149‑1 (MQ=255)
                                                                                                                       tggtTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACgtgg                         <  6:370848/149‑1 (MQ=255)
                                                                                                                        ggtTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACgtggt                        >  3:162385/1‑149 (MQ=255)
                                                                                                                          tttCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGc                      >  5:296769/1‑149 (MQ=255)
                                                                                                                              cTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGGGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCa                  <  6:41915/149‑1 (MQ=255)
                                                                                                                                 ccGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatga               >  3:111326/1‑149 (MQ=255)
                                                                                                                                   gtcacggcTGCCGCCCAGCGGCAGGCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGc                      >  8:340792/8‑140 (MQ=255)
                                                                                                                                   gtcacggcTGCCGCCCAGCGGCAGGCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGc                      <  7:340792/133‑1 (MQ=255)
                                                                                                                                    cgTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacga            <  6:290142/149‑1 (MQ=255)
                                                                                                                                    cgTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacga            <  7:320304/149‑1 (MQ=255)
                                                                                                                                      tCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgat           >  8:369695/1‑148 (MQ=255)
                                                                                                                                       cggcTGCCGCCCAGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgatga         >  3:294803/4‑149 (MQ=255)
                                                                                                                                        gCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgatga         <  4:162385/148‑1 (MQ=255)
                                                                                                                                           tGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGTGTCATGCCGATgacgac     >  7:183600/1‑149 (MQ=255)
                                                                                                                                           tGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATgacgac     >  4:218422/1‑149 (MQ=255)
                                                                                                                                             ccGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATgacgac     <  6:296769/147‑1 (MQ=255)
                                                                                                                                             ccGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATGACGAccc   >  4:127995/1‑149 (MQ=255)
                                                                                                                                             ccGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATGACGAccc   >  4:99417/1‑149 (MQ=255)
                                                                                                                                              cGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgatga         >  1:234672/1‑142 (MQ=255)
                                                                                                                                              cGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgatga         <  2:234672/142‑1 (MQ=255)
                                                                                                                                               gCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGAGGACGTCCCg  >  1:136891/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTGCCCGTGGTGGCCTGCGCTGGTCCGACCGCGAAGAGGACTTCCGTACCGAAGTGCT‑GGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGCGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATGACGACCCG  >  NC_002947/2369623‑2369912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑