Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,386,015 G→C 5.8% G363G (GGC→GGG)  PP_2094 ← nitrate‑binding protein NasS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,386,0150GC 5.8% 217.7 / 4.0 70G363G (GGC→GGG) PP_2094nitrate‑binding protein NasS
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base C (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGA  >  NC_002947/2385871‑2386158
                                                                                                                                                |                                                                                                                                               
gcAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTg                                                                                                                                             >  4:326950/1‑149 (MQ=255)
 cAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTgg                                                                                                                                            <  5:249453/149‑1 (MQ=255)
           gTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATg                                                                                                                                  >  4:122696/1‑149 (MQ=255)
           gTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATg                                                                                                                                  >  5:133645/1‑149 (MQ=255)
               cccTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGc                                                                                                                                                <  7:175169/131‑1 (MQ=255)
               cccTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGc                                                                                                                                                >  8:175169/1‑131 (MQ=255)
                ccTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGTAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCACCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAg                                                                                                                             <  5:359288/149‑1 (MQ=255)
                    aCGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGct                                                                                                                          <  1:140093/148‑1 (MQ=255)
                      gcgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtg                                                                                                                       >  5:227545/1‑149 (MQ=255)
                       cgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtgt                                                                                                                      <  7:95908/149‑1 (MQ=255)
                       cgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtgt                                                                                                                      <  2:58276/149‑1 (MQ=255)
                          cGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCg                                                                                                                   >  1:51348/1‑149 (MQ=255)
                           gACACGGGCATCGGGCGACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCgg                                                                                                                  <  7:234484/149‑1 (MQ=255)
                                gggCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCg                                                                                                             >  8:31780/1‑149 (MQ=255)
                                  gCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCt                                                                                                           <  8:350971/149‑1 (MQ=255)
                                   cATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTg                                                                                                          >  8:42545/1‑149 (MQ=255)
                                    aTCGTGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGGATATCCGAAGGTGTCGCTGCCGTCCCAGCGGGAGCCATCTATCAGCAGGCTGCTGCGCATCGGTTGTCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGt                                                                                                         <  4:308355/149‑1 (MQ=255)
                                    aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTg                                                                                                          >  7:260933/1‑148 (MQ=255)
                                    aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGt                                                                                                         <  8:48020/149‑1 (MQ=255)
                                    aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                  <  4:371327/124‑1 (MQ=255)
                                    aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                  <  4:377384/124‑1 (MQ=255)
                                    aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                  >  3:377384/1‑124 (MQ=255)
                                    aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                  >  3:371327/1‑124 (MQ=255)
                                         gCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAAc                                                                                                    >  4:253202/1‑149 (MQ=255)
                                             cTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTg                                                                                                  >  5:106445/1‑147 (MQ=255)
                                              tCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGACCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAg                                                                                               >  7:160902/1‑149 (MQ=255)
                                                 cccAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                            >  4:30671/1‑149 (MQ=255)
                                                  ccAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGc                                                                                           >  8:57077/1‑149 (MQ=255)
                                                     aGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTg                                                                                         >  8:368341/1‑148 (MQ=255)
                                                      ggCATGCAAGCTGAAACTGCGGGCCTAGCCGACTGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCCGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGa                                                                                       <  8:260933/149‑1 (MQ=255)
                                                       gCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGAc                                                                                      <  3:253202/149‑1 (MQ=255)
                                                          tGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTg                                                                                   >  3:42310/1‑149 (MQ=255)
                                                              aGCTGAACCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCgg                                                                               <  6:312098/149‑1 (MQ=255)
                                                               gCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCggg                                                                              >  3:189282/1‑149 (MQ=255)
                                                                cTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGc                                                                             <  6:227545/149‑1 (MQ=255)
                                                                 tGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGcc                                                                            >  6:53904/1‑149 (MQ=255)
                                                                   aaaCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCAc                                                                          <  2:51348/149‑1 (MQ=255)
                                                                    aaCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACg                                                                         <  7:31780/149‑1 (MQ=255)
                                                                     aCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGc                                                                        <  7:332564/149‑1 (MQ=255)
                                                                         cGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGGCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAg                                                                    <  1:279415/149‑1 (MQ=255)
                                                                         cGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAg                                                                    <  3:306944/149‑1 (MQ=255)
                                                                              aTAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                            >  5:25844/1‑120 (MQ=255)
                                                                              aTAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                            <  6:25844/120‑1 (MQ=255)
                                                                                 gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCg                                                                      >  6:157267/1‑139 (MQ=255)
                                                                                 gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCg                                                                      <  5:157267/139‑1 (MQ=255)
                                                                                 gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGa                                                                     <  4:359697/140‑1 (MQ=255)
                                                                                 gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGa                                                                     >  3:359697/1‑140 (MQ=255)
                                                                                 gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCgg                                                            <  3:326950/149‑1 (MQ=255)
                                                                                 gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCg                                                             <  3:242983/148‑1 (MQ=255)
                                                                                   cGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCggg                                                           <  4:231482/148‑1 (MQ=255)
                                                                                   cGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCggg                                                           >  3:231482/1‑148 (MQ=255)
                                                                                    gAAGGGGTCGCTGCCGTCCCAGAGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGt                                                         >  8:55263/1‑149 (MQ=255)
                                                                                          gTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTcgc                                                   <  3:30671/149‑1 (MQ=255)
                                                                                                 ccGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGcagcag                                            >  7:165827/1‑149 (MQ=255)
                                                                                                       cAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGccc                                        >  5:319462/1‑147 (MQ=255)
                                                                                                           gggAGCCATCGATCAGCAGGCGGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAgcgg                                  <  4:42310/149‑1 (MQ=255)
                                                                                                                  aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGGCCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                           >  8:193122/1‑149 (MQ=255)
                                                                                                                  aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                           <  7:368341/149‑1 (MQ=255)
                                                                                                                  aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                           >  1:368958/1‑149 (MQ=255)
                                                                                                                     gagcagcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTggg                        <  5:102506/146‑1 (MQ=255)
                                                                                                                        cagcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCa                     <  6:34826/149‑1 (MQ=255)
                                                                                                                          gcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCAt                    >  1:167301/1‑148 (MQ=255)
                                                                                                                                tgctgcGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACca              >  7:273549/1‑148 (MQ=255)
                                                                                                                                     cgcATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTTCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATAcc        <  7:55263/149‑1 (MQ=255)
                                                                                                                                        aTCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTc     >  8:258834/1‑149 (MQ=255)
                                                                                                                                        aTCGGGTGGCCTGGGCAAGCGATGCCCAGTCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTc     <  6:133645/149‑1 (MQ=255)
                                                                                                                                         tCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg    <  8:165827/149‑1 (MQ=255)
                                                                                                                                          cGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg    <  2:368958/148‑1 (MQ=255)
                                                                                                                                          cGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg    >  3:346134/1‑148 (MQ=255)
                                                                                                                                           ggTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGa  >  5:147056/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGA  >  NC_002947/2385871‑2386158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑