Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,386,021 G→C 6.0% C361W (TGC→TGG)  PP_2094 ← nitrate‑binding protein NasS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,386,0210GC 6.0% 208.6 / 4.5 67C361W (TGC→TGG) PP_2094nitrate‑binding protein NasS
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base C (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

GTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGACAGGTAGGG  >  NC_002947/2385882‑2386167
                                                                                                                                           |                                                                                                                                                  
gTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATg                                                                                                                                           >  4:122696/1‑149 (MQ=255)
gTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATg                                                                                                                                           >  5:133645/1‑149 (MQ=255)
     ccTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGTAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCACCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAg                                                                                                                                      <  5:359288/149‑1 (MQ=255)
         aCGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGct                                                                                                                                   <  1:140093/148‑1 (MQ=255)
           gcgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtg                                                                                                                                >  5:227545/1‑149 (MQ=255)
            cgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtgt                                                                                                                               <  2:58276/149‑1 (MQ=255)
            cgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtgt                                                                                                                               <  7:95908/149‑1 (MQ=255)
               cGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCg                                                                                                                            >  1:51348/1‑149 (MQ=255)
                gACACGGGCATCGGGCGACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCgg                                                                                                                           <  7:234484/149‑1 (MQ=255)
                     gggCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCg                                                                                                                      >  8:31780/1‑149 (MQ=255)
                       gCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCt                                                                                                                    <  8:350971/149‑1 (MQ=255)
                        cATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTg                                                                                                                   >  8:42545/1‑149 (MQ=255)
                         aTCGTGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGGATATCCGAAGGTGTCGCTGCCGTCCCAGCGGGAGCCATCTATCAGCAGGCTGCTGCGCATCGGTTGTCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGt                                                                                                                  <  4:308355/149‑1 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTg                                                                                                                   >  7:260933/1‑148 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGt                                                                                                                  <  8:48020/149‑1 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                           <  4:377384/124‑1 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                           >  3:371327/1‑124 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                           >  3:377384/1‑124 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                           <  4:371327/124‑1 (MQ=255)
                              gCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAAc                                                                                                             >  4:253202/1‑149 (MQ=255)
                                  cTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTg                                                                                                           >  5:106445/1‑147 (MQ=255)
                                   tCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGACCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAg                                                                                                        >  7:160902/1‑149 (MQ=255)
                                      cccAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                                     >  4:30671/1‑149 (MQ=255)
                                       ccAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGc                                                                                                    >  8:57077/1‑149 (MQ=255)
                                          aGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTg                                                                                                  >  8:368341/1‑148 (MQ=255)
                                           ggCATGCAAGCTGAAACTGCGGGCCTAGCCGACTGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCCGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGa                                                                                                <  8:260933/149‑1 (MQ=255)
                                            gCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGAc                                                                                               <  3:253202/149‑1 (MQ=255)
                                               tGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTg                                                                                            >  3:42310/1‑149 (MQ=255)
                                                   aGCTGAACCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCgg                                                                                        <  6:312098/149‑1 (MQ=255)
                                                    gCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCggg                                                                                       >  3:189282/1‑149 (MQ=255)
                                                     cTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGc                                                                                      <  6:227545/149‑1 (MQ=255)
                                                      tGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGcc                                                                                     >  6:53904/1‑149 (MQ=255)
                                                        aaaCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCAc                                                                                   <  2:51348/149‑1 (MQ=255)
                                                         aaCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACg                                                                                  <  7:31780/149‑1 (MQ=255)
                                                          aCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGc                                                                                 <  7:332564/149‑1 (MQ=255)
                                                              cGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGGCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAg                                                                             <  1:279415/149‑1 (MQ=255)
                                                              cGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAg                                                                             <  3:306944/149‑1 (MQ=255)
                                                                   aTAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                                     <  6:25844/120‑1 (MQ=255)
                                                                   aTAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                                     >  5:25844/1‑120 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCg                                                                               >  6:157267/1‑139 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCg                                                                               <  5:157267/139‑1 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGa                                                                              <  4:359697/140‑1 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGa                                                                              >  3:359697/1‑140 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCgg                                                                     <  3:326950/149‑1 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCg                                                                      <  3:242983/148‑1 (MQ=255)
                                                                        cGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCggg                                                                    <  4:231482/148‑1 (MQ=255)
                                                                        cGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCggg                                                                    >  3:231482/1‑148 (MQ=255)
                                                                         gAAGGGGTCGCTGCCGTCCCAGAGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGt                                                                  >  8:55263/1‑149 (MQ=255)
                                                                               gTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTcgc                                                            <  3:30671/149‑1 (MQ=255)
                                                                                      ccGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGcagcag                                                     >  7:165827/1‑149 (MQ=255)
                                                                                            cAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGccc                                                 >  5:319462/1‑147 (MQ=255)
                                                                                                gggAGCCATCGATCAGCAGGCGGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAgcgg                                           <  4:42310/149‑1 (MQ=255)
                                                                                                       aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGGCCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                                    >  8:193122/1‑149 (MQ=255)
                                                                                                       aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                                    <  7:368341/149‑1 (MQ=255)
                                                                                                       aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                                    >  1:368958/1‑149 (MQ=255)
                                                                                                          gagcagcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTggg                                 <  5:102506/146‑1 (MQ=255)
                                                                                                             cagcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCa                              <  6:34826/149‑1 (MQ=255)
                                                                                                               gcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCAt                             >  1:167301/1‑148 (MQ=255)
                                                                                                                     tgctgcGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACca                       >  7:273549/1‑148 (MQ=255)
                                                                                                                          cgcATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTTCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATAcc                 <  7:55263/149‑1 (MQ=255)
                                                                                                                             aTCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTc              >  8:258834/1‑149 (MQ=255)
                                                                                                                             aTCGGGTGGCCTGGGCAAGCGATGCCCAGTCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTc              <  6:133645/149‑1 (MQ=255)
                                                                                                                              tCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg             <  8:165827/149‑1 (MQ=255)
                                                                                                                               cGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg             <  2:368958/148‑1 (MQ=255)
                                                                                                                               cGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg             >  3:346134/1‑148 (MQ=255)
                                                                                                                                ggTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGa           >  5:147056/1‑149 (MQ=255)
                                                                                                                                      ccTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTGGGACAgg       >  8:213490/1‑147 (MQ=255)
                                                                                                                                         gggCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGACAGGTAggg  >  6:24076/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
GTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGACAGGTAGGG  >  NC_002947/2385882‑2386167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑