Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,386,025 G→C 5.8% A360G (GCT→GGT)  PP_2094 ← nitrate‑binding protein NasS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,386,0250GC 5.8% 223.7 / 3.2 69A360G (GCT→GGT) PP_2094nitrate‑binding protein NasS
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base C (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGACAGGTAGGGCAG  >  NC_002947/2385882‑2386170
                                                                                                                                               |                                                                                                                                                 
gTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATg                                                                                                                                              >  4:122696/1‑149 (MQ=255)
gTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATg                                                                                                                                              >  5:133645/1‑149 (MQ=255)
     ccTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGTAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCACCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAg                                                                                                                                         <  5:359288/149‑1 (MQ=255)
         aCGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGct                                                                                                                                      <  1:140093/148‑1 (MQ=255)
           gcgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtg                                                                                                                                   >  5:227545/1‑149 (MQ=255)
            cgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtgt                                                                                                                                  <  2:58276/149‑1 (MQ=255)
            cgcCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCtgt                                                                                                                                  <  7:95908/149‑1 (MQ=255)
               cGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCg                                                                                                                               >  1:51348/1‑149 (MQ=255)
                gACACGGGCATCGGGCGACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCgg                                                                                                                              <  7:234484/149‑1 (MQ=255)
                     gggCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCg                                                                                                                         >  8:31780/1‑149 (MQ=255)
                       gCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCt                                                                                                                       <  8:350971/149‑1 (MQ=255)
                        cATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTg                                                                                                                      >  8:42545/1‑149 (MQ=255)
                         aTCGTGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGGATATCCGAAGGTGTCGCTGCCGTCCCAGCGGGAGCCATCTATCAGCAGGCTGCTGCGCATCGGTTGTCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGt                                                                                                                     <  4:308355/149‑1 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTg                                                                                                                      >  7:260933/1‑148 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGt                                                                                                                     <  8:48020/149‑1 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                              <  4:377384/124‑1 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                              >  3:371327/1‑124 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                              >  3:377384/1‑124 (MQ=255)
                         aTCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGCTTGCCCAGGCCAACCGATg                                                                                                                                              <  4:371327/124‑1 (MQ=255)
                              gCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAAc                                                                                                                >  4:253202/1‑149 (MQ=255)
                                  cTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTg                                                                                                              >  5:106445/1‑147 (MQ=255)
                                   tCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGACCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAg                                                                                                           >  7:160902/1‑149 (MQ=255)
                                      cccAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                                        >  4:30671/1‑149 (MQ=255)
                                       ccAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGc                                                                                                       >  8:57077/1‑149 (MQ=255)
                                          aGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTg                                                                                                     >  8:368341/1‑148 (MQ=255)
                                           ggCATGCAAGCTGAAACTGCGGGCCTAGCCGACTGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCCGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGa                                                                                                   <  8:260933/149‑1 (MQ=255)
                                            gCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGAc                                                                                                  <  3:253202/149‑1 (MQ=255)
                                               tGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTg                                                                                               >  3:42310/1‑149 (MQ=255)
                                                   aGCTGAACCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCgg                                                                                           <  6:312098/149‑1 (MQ=255)
                                                    gCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCggg                                                                                          >  3:189282/1‑149 (MQ=255)
                                                     cTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGc                                                                                         <  6:227545/149‑1 (MQ=255)
                                                      tGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGcc                                                                                        >  6:53904/1‑149 (MQ=255)
                                                        aaaCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCAc                                                                                      <  2:51348/149‑1 (MQ=255)
                                                         aaCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACg                                                                                     <  7:31780/149‑1 (MQ=255)
                                                          aCTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGc                                                                                    <  7:332564/149‑1 (MQ=255)
                                                              cGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGGCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAg                                                                                <  1:279415/149‑1 (MQ=255)
                                                              cGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAg                                                                                <  3:306944/149‑1 (MQ=255)
                                                                   aTAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                                        <  6:25844/120‑1 (MQ=255)
                                                                   aTAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTg                                                                                                        >  5:25844/1‑120 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCg                                                                                  >  6:157267/1‑139 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCg                                                                                  <  5:157267/139‑1 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGa                                                                                 <  4:359697/140‑1 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGa                                                                                 >  3:359697/1‑140 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCgg                                                                        <  3:326950/149‑1 (MQ=255)
                                                                      gCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCg                                                                         <  3:242983/148‑1 (MQ=255)
                                                                        cGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCggg                                                                       >  3:231482/1‑148 (MQ=255)
                                                                        cGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCggg                                                                       <  4:231482/148‑1 (MQ=255)
                                                                         gAAGGGGTCGCTGCCGTCCCAGAGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGt                                                                     >  8:55263/1‑149 (MQ=255)
                                                                               gTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTcgc                                                               <  3:30671/149‑1 (MQ=255)
                                                                                      ccGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGcagcag                                                        >  7:165827/1‑149 (MQ=255)
                                                                                            cAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGccc                                                    >  5:319462/1‑147 (MQ=255)
                                                                                                gggAGCCATCGATCAGCAGGCGGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAgcgg                                              <  4:42310/149‑1 (MQ=255)
                                                                                                       aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGGCCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                                       >  8:193122/1‑149 (MQ=255)
                                                                                                       aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                                       <  7:368341/149‑1 (MQ=255)
                                                                                                       aTCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACt                                       >  1:368958/1‑149 (MQ=255)
                                                                                                          gagcagcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTggg                                    <  5:102506/146‑1 (MQ=255)
                                                                                                             cagcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCa                                 <  6:34826/149‑1 (MQ=255)
                                                                                                               gcagGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCAt                                >  1:167301/1‑148 (MQ=255)
                                                                                                                     tgctgcGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACca                          >  7:273549/1‑148 (MQ=255)
                                                                                                                          cgcATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTTCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATAcc                    <  7:55263/149‑1 (MQ=255)
                                                                                                                             aTCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTc                 >  8:258834/1‑149 (MQ=255)
                                                                                                                             aTCGGGTGGCCTGGGCAAGCGATGCCCAGTCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTc                 <  6:133645/149‑1 (MQ=255)
                                                                                                                              tCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg                <  8:165827/149‑1 (MQ=255)
                                                                                                                               cGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg                <  2:368958/148‑1 (MQ=255)
                                                                                                                               cGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCg                >  3:346134/1‑148 (MQ=255)
                                                                                                                                ggTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGa              >  5:147056/1‑149 (MQ=255)
                                                                                                                                      ccTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTGGGACAgg          >  8:213490/1‑147 (MQ=255)
                                                                                                                                         gggCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGACAGGTAggg     >  6:24076/1‑149 (MQ=255)
                                                                                                                                            cAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGACAGGTAGGGCAg  <  8:160902/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCGGGCATAGCCGAAGGGGTCGCTGCCGTCCCAGCGGGAGCCATCGATCAGCAGGCTGCTGCGCATCGGTTGGCCTGGGCAAGCGATGCCCAGGCTCTGTGCGGCCTCGCTGTACAACTGCAGTTGCTGGACCTGCCGGGCCACGCCGAGGTAGTCGGGGTCTTCGCGCAGCAGGCCCCAGCGGCGGAACTGGGTCATGAACCACATACCGTCGGACAGGTAGGGCAG  >  NC_002947/2385882‑2386170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑