Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,441,066	G→T	6.1%	K68N (AAG→AAT)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,441,066	0	G	T	6.1%	213.5 / 3.5	66	K68N (AAG→AAT)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base G (37/25);  new base T (2/2);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

TACCTGGGCAACCAGTACGTGGTGAAGTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTCGCAACCGACT  >  NC_002947/2440920‑2441214                                                                                                                                                     |                                                                                                                                                     tACCTGGACAACCAGTACGTGGTGAAGTCGAGTATCGGCCATATCCGAGACCTACCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGc                                                                                                                                                    >  7:316198/1‑149 (MQ=255)   ccTGGGCAACCAGTACGTGGTGAAGTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGccc                                                                                                                                                  >  3:48303/1‑149 (MQ=255)   ccTGGGCAACCAGTACGTGGTGAAGTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGc                                                                                                                                                    <  5:350997/147‑1 (MQ=255)    cTGGGCAACCAGTACGTGGTGAAGTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCccg                                                                                                                                                 >  4:298638/1‑149 (MQ=255)     tGGGCAACCAGTACGTGGTGAAGTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCccgc                                                                                                                                                >  4:248444/1‑149 (MQ=255)             cAGTACGTGGTGAAGTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGCTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCt                                                                                                                                        >  5:44468/1‑149 (MQ=255)                        gAAGTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCa                                                                                                                             >  6:214854/1‑149 (MQ=255)                           gTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATgg                                                                                                                          >  4:136814/1‑149 (MQ=255)                                gTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTc                                                                                                                     <  8:302356/149‑1 (MQ=255)                                 tATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTc                                                                                                                     >  5:211891/1‑148 (MQ=255)                                      gCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGAcccc                                                                                                               >  1:383675/1‑149 (MQ=255)                                        cATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGa                                                                                                             <  3:298638/149‑1 (MQ=255)                                            tCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCAt                                                                                                         >  2:70998/1‑149 (MQ=255)                                              cGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATgg                                                                                                       >  7:101829/1‑149 (MQ=255)                                               gagaCCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCTGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGc                                                                                                      <  1:70998/149‑1 (MQ=255)                                                  aCCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGCAAGAGAAGGCCCGCGGCCACCGGTTGGCCCCCATGGGCGTCCCCCCCGCCCATGCCTg                                                                                                    >  3:335224/1‑148 (MQ=255)                                                    cTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGTCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGaa                                                                                                 <  1:180660/149‑1 (MQ=255)                                                    cTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTgg                                                                                                   >  7:354985/1‑147 (MQ=255)                                                    cTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGaa                                                                                                 <  7:259676/149‑1 (MQ=255)                                                     tCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAATGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAg                                                                                                >  7:237042/1‑149 (MQ=255)                                                     tCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAATGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAg                                                                                                >  7:230910/1‑149 (MQ=255)                                                     tCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCAGGCATGGGCGTCGACCCCGACCATGGCTGGCAg                                                                                                >  4:115756/1‑149 (MQ=255)                                                     tCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTc                                                                                                                     >  2:285714/1‑128 (MQ=255)                                                     tCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTc                                                                                                                     <  1:285714/128‑1 (MQ=255)                                                     tCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAg                                                                                                >  7:118670/1‑149 (MQ=255)                                                      ccccACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGCCCATGGCTGGAAgg                                                                                               >  5:301076/1‑149 (MQ=255)                                                       cccACCAGCGGTTCGGCCAGCGCGCGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGATTTCGCCGAAAGAGAAGGCCCGCCGCCAGCGGGTGGCACGCATGGGCGTCGACCCCGCCCATGGCGGGAAGGc                                                                                              >  1:119205/1‑149 (MQ=255)                                                        ccaccaGCGGTTCGGCCAGCGCGAGCAAGAGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGc                                                                                              >  1:23828/1‑149 (MQ=255)                                                          accaGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGcc                                                                                             >  6:61270/1‑147 (MQ=255)                                                           ccaGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGCTCAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGcc                                                                                             >  1:68879/1‑147 (MQ=255)                                                              gCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTAc                                                                                       >  3:82131/1‑149 (MQ=255)                                                                ggTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACga                                                                                     <  3:248444/149‑1 (MQ=255)                                                                        cAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATcctgc                                                                             <  4:48303/149‑1 (MQ=255)                                                                           cgcgAGCAA‑AGAGCCGGCCGC‑CAATCGTGGCAAGGCTGCTGGTGAGGCGCCGGGGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATcctgcctg                                                                          <  6:44468/149‑1 (MQ=255)                                                                            gcgAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTgg                                                                         >  4:127661/1‑149 (MQ=255)                                                                              gAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGc                                                                        >  4:22277/1‑148 (MQ=255)                                                                               aGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCaa                                                                      <  2:383675/149‑1 (MQ=255)                                                                                gCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAATGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAg                                                                     <  8:237042/149‑1 (MQ=255)                                                                                gCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAATGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAg                                                                     <  8:230910/149‑1 (MQ=255)                                                                                     agagCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGaa                                                                  >  5:209981/1‑148 (MQ=255)                                                                                        gccggccgC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAg                                                               <  3:136814/148‑1 (MQ=255)                                                                                           ggccgC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGa                                                           <  5:88478/149‑1 (MQ=255)                                                                                           ggccgC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACAAGATCCTGCCTGGCAAGGAAAAGGTGa                                                           <  8:101829/149‑1 (MQ=255)                                                                                            gccgc‑cAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGAt                                                          <  8:316198/149‑1 (MQ=255)                                                                                               gc‑cAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCg                                                        >  6:74044/1‑148 (MQ=255)                                                                                                  cAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCg                                                        >  8:58013/1‑146 (MQ=255)                                                                                                  cAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCg                                                        >  5:365254/1‑146 (MQ=255)                                                                                                   aaGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAaga                                                    <  2:68879/149‑1 (MQ=255)                                                                                                    aGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAagag                                                   <  5:214854/149‑1 (MQ=255)                                                                                                            aaGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGcgccg                                           >  8:155745/1‑149 (MQ=255)                                                                                                            aaGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGCCGTCGACCCCGACCATGGCTGGAACGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGcgccg                                           >  8:314019/1‑149 (MQ=255)                                                                                                             aGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGcgccgc                                          <  4:82131/149‑1 (MQ=255)                                                                                                                      gggAGGCGCCGGCGCGTTCGCCGAAAGAGAAGGCCCGCCGCCCGCTGGTGGCACGCATGGGCGTCGACCCCTACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGACGAGCTGCGCCGCCTGGCCAAg                                 <  2:119205/147‑1 (MQ=255)                                                                                                                           gcgcCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGc                            <  5:61270/149‑1 (MQ=255)                                                                                                                                     tttCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCGGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATc                  <  8:354985/149‑1 (MQ=255)                                                                                                                                       tCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCt                 <  3:127661/148‑1 (MQ=255)                                                                                                                                         gCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACAcc                     >  7:192570/1‑142 (MQ=255)                                                                                                                                         gCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACAcc                     <  8:192570/142‑1 (MQ=255)                                                                                                                                           cGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTc            >  1:334079/1‑149 (MQ=255)                                                                                                                                               agagAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTCGCaa        <  7:314019/149‑1 (MQ=255)                                                                                                                                                 agaAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTCGCAAcc      >  3:250833/1‑149 (MQ=255)                                                                                                                                                 agaAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTCGCAAcc      >  6:225947/1‑149 (MQ=255)                                                                                                                                                 agaAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTCGCAAcc      >  5:370941/1‑149 (MQ=255)                                                                                                                                                 agaAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGACGCCGACACCAt                   >  6:61303/1‑136 (MQ=255)                                                                                                                                                 agaAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGACGCCGACACCAt                   <  5:61303/136‑1 (MQ=255)                                                                                                                                                  gaAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTCGCAAcc      >  7:110633/1‑148 (MQ=255)                                                                                                                                                     ggCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTCGCAACCGACt  >  8:157678/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TACCTGGGCAACCAGTACGTGGTGAAGTCGAGTATCGGCCATATCCGAGACCTCCCCACCAGCGGTTCGGCCAGCGCGAGCAA‑AGAGCCGGCCGC‑CAAGCGTGGCAAGGCTGCGGGTGAGGCGCCGGCGCTTTCGCCGAAAGAGAAGGCCCGCCGCCAGCTGGTGGCACGCATGGGCGTCGACCCCGACCATGGCTGGAAGGCCAAGTACGAGATCCTGCCTGGCAAGGAAAAGGTGATCGAAGAGCTGCGCCGCCTGGCCAAGGATGCCGACACCATCTACCTCGCAACCGACT  >  NC_002947/2440920‑2441214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑