Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,455,666 T→C 6.5% G127G (GGT→GGC)  gapB → glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,455,6660TC 6.5% 168.8 / 2.3 61G127G (GGT→GGC) gapBglyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (31/26);  new base C (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

GACATTCCCCCTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATCGTCGTGCGCAAAGG  >  NC_002947/2455519‑2455809
                                                                                                                                                   |                                                                                                                                               
gaCATTCCCCCTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTggtcggt                                                                                                                                                 <  5:205338/148‑1 (MQ=255)
        cccTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAAc                                                                                                                                        <  8:296965/149‑1 (MQ=255)
        cccTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAAc                                                                                                                                        >  1:101206/1‑149 (MQ=255)
         ccTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGCCCGACGTGGTCGGTCAGCAGAACg                                                                                                                                       >  2:216977/1‑149 (MQ=255)
                     cATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCAGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGgcgcgc                                                                                                                            >  2:309289/1‑148 (MQ=255)
                       tGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGaa                                                                                                                                         <  6:355558/133‑1 (MQ=255)
                       tGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGaa                                                                                                                                         >  5:355558/1‑133 (MQ=255)
                           cgagcTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGCCCGACGTGGTCGGTTAGCAGAACGCTTCGGCGCGCAAAGGCa                                                                                                                     <  1:216977/149‑1 (MQ=255)
                            gagcTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCAc                                                                                                                    <  1:335823/149‑1 (MQ=255)
                                  aaaCTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGAc                                                                                                                <  3:383251/147‑1 (MQ=255)
                                   aaCTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGtt                                                                                                             >  6:47835/1‑149 (MQ=255)
                                   aaCTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGtt                                                                                                             <  5:219282/149‑1 (MQ=255)
                                      tGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGGTGTc                                                                                                          >  8:15914/1‑149 (MQ=255)
                                        ggCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTc                                                                                                          <  7:365425/147‑1 (MQ=255)
                                        ggCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTc                                                                                                          <  6:254249/147‑1 (MQ=255)
                                        ggCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTc                                                                                                          <  3:65835/147‑1 (MQ=255)
                                        ggCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCt                                                                                                        <  1:313025/149‑1 (MQ=255)
                                            ccgcATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTAc                                                                                                    >  7:370405/1‑149 (MQ=255)
                                             cgcATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACg                                                                                                   >  2:78133/1‑149 (MQ=255)
                                             cgcATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACg                                                                                                   >  8:41535/1‑149 (MQ=255)
                                             cgcATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACg                                                                                                   >  3:59527/1‑149 (MQ=255)
                                                aTCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCt                                                                                                >  2:226288/1‑149 (MQ=255)
                                                 tCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCtt                                                                                               <  8:108386/149‑1 (MQ=255)
                                                          cTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCAt                                                                                      >  7:268745/1‑149 (MQ=255)
                                                            gggCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCg                                                                                    >  7:314600/1‑149 (MQ=255)
                                                                aaGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGgccg                                                                                >  4:222774/1‑149 (MQ=255)
                                                                  gCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGgccgcc                                                                              <  7:41535/149‑1 (MQ=255)
                                                                      gCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCctgct                                                                          >  5:2828/1‑149 (MQ=255)
                                                                      gCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAACGCACCGACGTTATACTGTACGGCTTCGGTCGCATCGTCCGCctgct                                                                          >  7:348783/1‑149 (MQ=255)
                                                                           caaGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGgcgc                                                                     <  1:78133/149‑1 (MQ=255)
                                                                            aaGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGgcgcg                                                                    >  4:273265/1‑149 (MQ=255)
                                                                            aaGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGgcgcg                                                                    >  6:98003/1‑149 (MQ=255)
                                                                            aaGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGgcgcg                                                                    >  1:157139/1‑149 (MQ=255)
                                                                               ttCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCAt                                                                 >  5:137916/1‑149 (MQ=255)
                                                                                 cAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATc                                                                <  4:59527/148‑1 (MQ=255)
                                                                                    gCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTgg                                                                        <  2:380587/137‑1 (MQ=255)
                                                                                    gCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTgg                                                                        >  1:380587/1‑137 (MQ=255)
                                                                                      aGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATc                                                          <  2:101206/149‑1 (MQ=255)
                                                                                        gAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGgc                                                                       >  7:250486/1‑134 (MQ=255)
                                                                                        gAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGgc                                                                       <  8:250486/134‑1 (MQ=255)
                                                                                          aGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCgaga                                                      <  5:98003/149‑1 (MQ=255)
                                                                                          aGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCgaga                                                      <  3:273265/149‑1 (MQ=255)
                                                                                              aaTGGCCGCACTGCCGAGCAGTTCTTCCCTGAAGAGCTGGCCGACGTGGACGGTCAGCAGAACGCTTCGTCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCTGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGAc                                                  >  4:317894/1‑149 (MQ=255)
                                                                                                   ccGCACTGCTGAGGAGTTCGTCCGTGAAGATCTGGCCGACGTGGTCTGTCAGCATAACGCTTCGGCGCGCAAAGTCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCggtg                                             <  8:348783/149‑1 (MQ=255)
                                                                                                   ccGCACTGCCGTGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCATAACGCTTAGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCggtg                                             <  4:56981/149‑1 (MQ=255)
                                                                                                   ccGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGTAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATc                                                                <  1:9886/130‑1 (MQ=255)
                                                                                                   ccGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGTAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATc                                                                >  2:9886/1‑130 (MQ=255)
                                                                                                      cACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCggtggtg                                          >  4:352814/1‑149 (MQ=255)
                                                                                                             gAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGGGCATCCTGATCGAGAAGACCGGTGGTGGTGACgg                                   >  8:362785/1‑149 (MQ=255)
                                                                                                              aGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGc                                  <  1:226288/149‑1 (MQ=255)
                                                                                                                     cGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCctgcgtc                           >  2:31990/1‑149 (MQ=255)
                                                                                                                     cGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCctgcgt                            <  8:314600/148‑1 (MQ=255)
                                                                                                                      gTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCctgcgtct                          >  1:11028/1‑149 (MQ=255)
                                                                                                                          gTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCctgcgtctgcgt                      >  8:118614/1‑149 (MQ=255)
                                                                                                                            gttctgCTGACCGACCACGTCGGCCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCctgcgtct                          <  8:16015/138‑1 (MQ=255)
                                                                                                                            gttctgCTGACCGACCACGTCGGCCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCctgcgtct                          >  7:16015/6‑143 (MQ=255)
                                                                                                                             ttctgCTGACCGACCACGTCGGCCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCctgcgtctg                         <  3:188659/139‑1 (MQ=255)
                                                                                                                             ttctgCTGACCGACCACGTCGGCCAGCAGAACGCTTCGGAGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCctgcgtctg                         >  4:188659/5‑143 (MQ=255)
                                                                                                                                     gCCTACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCACAGGCACCGACCTGGTCCTGAACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATcgtcgt           <  8:311454/149‑1 (MQ=255)
                                                                                                                                     gCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATcgtcgt           <  5:47835/149‑1 (MQ=255)
                                                                                                                                         aCGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATCGTCGTgcg        >  3:212553/1‑148 (MQ=255)
                                                                                                                                           gTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATCATCGTGCGCa      >  3:3294/1‑148 (MQ=255)
                                                                                                                                              gtcggtcAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATCGTCGTGCGCAAAg   <  3:267718/148‑1 (MQ=255)
                                                                                                                                              gtcggtcAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATCGTCGTGCGCAAAg   >  4:267718/1‑148 (MQ=255)
                                                                                                                                              gtcggtcAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCACATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATCGTCGTGCGCAAAgg  >  5:127259/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GACATTCCCCCTGCTCAAGGCCATGAGCGAGCTGAAACTGGGCGCCGCATCGGTCGACCTGGGCAAGCTGGCCAACAAGTTCAAGCAGGAAGGCAATGGCCGCACTGCCGAGCAGTTCGTCCGTGAAGAGCTGGCCGACGTGGTCGGTCAGCAGAACGCTTCGGCGCGCAAAGGCACCGACGTTGTCCTGTACGGCTTCGGTCGCATCGGCCGCCTGCTGGCGCGCATCCTGATCGAGAAGACCGGTGGTGGTGACGGCCTGCGTCTGCGTGCCATCGTCGTGCGCAAAGG  >  NC_002947/2455519‑2455809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑