Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,475,286 T→G 7.8% intergenic (‑85/+72) tal ← / ← dusA transaldolase/tRNA dihydrouridine synthase DusA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,475,2860TG 7.8% 154.8 / 4.4 51intergenic (‑85/+72)tal/dusAtransaldolase/tRNA dihydrouridine synthase DusA
Reads supporting (aligned to +/- strand):  ref base T (25/22);  new base G (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

CCAGGTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAATGTC  >  NC_002947/2475138‑2475426
                                                                                                                                                    |                                                                                                                                            
ccAGGTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCt                                                                                                                                              >  7:360567/1‑149 (MQ=255)
  aGGTCCCCGGTGGCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGGCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTgg                                                                                                                                            <  7:114123/149‑1 (MQ=255)
    gTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGcc                                                                                                                                          >  7:7131/1‑149 (MQ=255)
    gTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGcc                                                                                                                                          <  8:28970/149‑1 (MQ=255)
     gccccGGTGGCGGCGACCACGGGGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCAATTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGccc                                                                                                                                         <  6:70736/148‑1 (MQ=255)
       cccGGTGTCGGCTACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTGGGAGGTCATGGGCGTGCGCTGTCCTGCGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGGCCAGCGGGTCCGc                                                                                                                                       <  4:182965/149‑1 (MQ=255)
        ccGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGca                                                                                                                                      >  6:349156/1‑149 (MQ=255)
               cGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCt                                                                                                                               <  5:172607/149‑1 (MQ=255)
                       aCGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGa                                                                                                                         >  7:243990/1‑147 (MQ=255)
                          gtggtgAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGt                                                                                                                    <  8:243990/149‑1 (MQ=255)
                           tggtgAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGt                                                                                                                    <  6:51534/148‑1 (MQ=255)
                           tggtgAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGt                                                                                                                    >  5:51534/1‑148 (MQ=255)
                             gtgAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGa                                                                                                                  <  5:234120/148‑1 (MQ=255)
                                  cTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCGCGCACAGGGCt                                                                                                                               >  6:245215/1‑130 (MQ=255)
                                  cTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCGCGCACAGGGCt                                                                                                                               <  5:245215/130‑1 (MQ=255)
                                     cTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGa                                                                                                          <  1:40939/148‑1 (MQ=255)
                                     cTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGa                                                                                                          <  5:349156/148‑1 (MQ=255)
                                     cTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGa                                                                                                          <  8:211188/148‑1 (MQ=255)
                                       tGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTAGACATTACCCGAGACCCGGCAGCCGCTCAAGCGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTc                                                                                                       >  1:298539/1‑149 (MQ=255)
                                        gAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTc                                                                                                       <  7:130772/148‑1 (MQ=255)
                                        gAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTc                                                                                                       >  8:130772/1‑148 (MQ=255)
                                                ccAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGGCCAGCGGGTCCGca                                                                                                                                      <  6:264866/109‑1 (MQ=255)
                                                ccAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGGCCAGCGGGTCCGca                                                                                                                                      >  5:264866/1‑109 (MQ=255)
                                                ccAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGcaagcaag                                                                                              >  2:259402/1‑149 (MQ=255)
                                                 cAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCaagcaagc                                                                                             <  1:255367/149‑1 (MQ=255)
                                                      tGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAg                                                                                        >  3:83523/1‑149 (MQ=255)
                                                                     tGCTCTGTCCTGTGCATTTACTCTACAATACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGTTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGa                                                                         <  8:360567/149‑1 (MQ=255)
                                                                       ctctGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGTACCCGCTGGCCCGCACAGGGCTGTACGAAAg                                                                                                                      >  5:278290/1‑102 (MQ=255)
                                                                       ctctGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAg                                                                                                                      <  6:278290/102‑1 (MQ=255)
                                                                                   cATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTtccctc                                                                                <  4:354794/128‑1 (MQ=255)
                                                                                   cATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTtccctc                                                                                >  3:354794/1‑128 (MQ=255)
                                                                                    aTTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTg                                                           >  8:256300/1‑148 (MQ=255)
                                                                                        aCTGGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTTAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGcaac                                                       <  2:298539/148‑1 (MQ=255)
                                                                                                   cccGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCCCCGCCCCTGCAACAACTCCACGGcc                                           >  5:128190/1‑149 (MQ=255)
                                                                                                    ccGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCt                                          >  8:94761/1‑149 (MQ=255)
                                                                                                     cGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTg                                         <  4:83523/149‑1 (MQ=255)
                                                                                                           ccGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCaaa                                   >  6:153163/1‑149 (MQ=255)
                                                                                                                   cGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGAGTCGAGTCGCAAGCCAGCCCGCGGTTCCCTCCCTCTGAGGGCACAGCCCCTGCAACAACTCCACGGCCTGGTCACGCACCGcc                            >  4:224848/1‑148 (MQ=255)
                                                                                                                        aaGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTTAAGATTCTAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAgg                        <  3:142856/147‑1 (MQ=255)
                                                                                                                        aaGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGt                      >  3:178881/1‑149 (MQ=255)
                                                                                                                             ccgccgAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAgc                 >  3:27491/1‑149 (MQ=255)
                                                                                                                                ccgAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAgccgc              >  5:290965/1‑149 (MQ=255)
                                                                                                                                  gAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAgc                 >  6:382383/1‑144 (MQ=255)
                                                                                                                                  gAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAgc                 <  5:382383/144‑1 (MQ=255)
                                                                                                                                    ccctgtGCGGGCCAGCGGGTCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTtg          >  1:176381/6‑149 (MQ=255)
                                                                                                                                      cccTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTtgt         >  5:334628/1‑148 (MQ=255)
                                                                                                                                         tGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAAt     >  2:68231/1‑149 (MQ=255)
                                                                                                                                         tGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTACCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACAGCCAAGCGTACAGCCGCCTTGTGAAt     >  8:241296/1‑149 (MQ=255)
                                                                                                                                           cGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAAt     <  1:259402/147‑1 (MQ=255)
                                                                                                                                            ggACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAATGTc  >  4:356477/1‑149 (MQ=255)
                                                                                                                                                 cGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTg                                                           >  2:179901/1‑87 (MQ=255)
                                                                                                                                                 cGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTg                                                           <  1:179901/87‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CCAGGTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAATGTC  >  NC_002947/2475138‑2475426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑