Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,515,477 C→A 6.5% A351S (GCC→TCC)  phnW ← 2‑aminoethylphosphonate‑‑pyruvate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,515,4770CA 6.5% 194.1 / 2.9 61A351S (GCC→TCC) phnW2‑aminoethylphosphonate‑‑pyruvate transaminase
Reads supporting (aligned to +/- strand):  ref base C (22/35);  new base A (2/2);  total (24/37)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GGTGCCAGCCCAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCGGGCAT  >  NC_002947/2515330‑2515611
                                                                                                                                                    |                                                                                                                                      
ggTGCCAGCCCAGTCGAGGATGGCGGCTTGCAGTTGGGTGCGGTTGTTGTAGTGCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGcc                                                                                                                                       >  1:357917/1‑149 (MQ=255)
 gTGCCAGCCCAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCt                                                                                                                                      <  2:17273/149‑1 (MQ=255)
      aGCCCAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATg                                                                                                                                 <  6:374763/149‑1 (MQ=255)
       gCCCAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGc                                                                                                                                >  3:75213/1‑149 (MQ=255)
       gCCCAGGCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGc                                                                                                                                >  8:271523/1‑149 (MQ=255)
        cccAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGcc                                                                                                                               <  8:38063/149‑1 (MQ=255)
         ccAGTCGAGGATGGCGGCTTTCAGTTGGTGGGGGATGTTGTAGATCGTGTGCGCAAACTCCTGAATTCGCGTGGGCAAAGCGTCAGAAATCCAGCACTTCCAATTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCg                                                                                                                              <  5:397086/149‑1 (MQ=255)
          cAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTTGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGt                                                                                                                             <  8:272850/149‑1 (MQ=255)
               gAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGGACGGCATGCAGGCCgccgt                                                                                                                          <  2:302139/148‑5 (MQ=255)
               gAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATACACGG‑CGGCCTGCATGCCGTCCGc                                                                                                                         <  3:206998/148‑1 (MQ=255)
                    tcgcggcTTTCAGTTTGGTGGGGGTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGAc                                                                                                                   <  8:178931/147‑1 (MQ=255)
                    tGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGAc                                                                                                                   <  6:391575/149‑1 (MQ=255)
                        ggcTTGCAGTTGGGTGTGGGTGTTGTAGTTCATGTGCGCTTACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCGACGTCATTCACGG‑CGGCCTGCATGCCGTCCTCCCCGACCAct                                                                                                               <  2:357917/149‑2 (MQ=255)
                           ttGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGcc                                                                                                             >  2:175717/1‑148 (MQ=255)
                                gTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCa                                                                                                       <  8:63949/149‑1 (MQ=255)
                                         ggTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGACGGCATGCAGgccgccgtga                                                                                                                    >  1:83371/1‑123 (MQ=255)
                                         ggTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGACGGCATGCAGgccgccgtga                                                                                                                    <  2:83371/127‑5 (MQ=255)
                                          gttgttGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGACGGCATGCAGgccgccgtg                                                                                                                     >  5:391266/1‑122 (MQ=255)
                                          gttgttGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGACGGCATGCAGgccgccgtg                                                                                                                     <  6:391266/125‑4 (MQ=255)
                                           ttgttgTAGTTAATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCgg                                                                                             >  4:71111/1‑148 (MQ=255)
                                                gtAGTTAATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGAACTTCCATTTCCCGCAGCACCTCGGACACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCACCCACCACGCCGATGCAGCCGACGCGGAAGGtt                                                                                       >  6:61538/1‑149 (MQ=255)
                                                   gTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTc                                                                                     <  4:75213/148‑1 (MQ=255)
                                                      cATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGAc                                                                                  <  6:308488/148‑1 (MQ=255)
                                                          tgCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTgg                                                                              <  1:72052/148‑1 (MQ=255)
                                                          tgCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTgg                                                                              <  3:71111/148‑1 (MQ=255)
                                                          tgCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTgg                                                                              <  1:271353/148‑1 (MQ=255)
                                                           gcgcTAACTCCTGAATTCGGGTGGTCAAAGCGTCAGATATCCAGCACTTCCATTTCCCTCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGt                                                                            <  7:286887/149‑1 (MQ=255)
                                                               tAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAAc                                                                        >  8:108785/1‑149 (MQ=255)
                                                                 aCTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAAc                                                                        <  5:392663/147‑1 (MQ=255)
                                                                 aCTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAAc                                                                        <  1:248110/147‑1 (MQ=255)
                                                                 aCTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACtt                                                                      <  3:286572/149‑1 (MQ=255)
                                                                 aCTCCAGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAAc                                                                        <  7:271523/147‑1 (MQ=255)
                                                                   tCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGc                                                                    >  1:104913/1‑149 (MQ=255)
                                                                   tCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGc                                                                    >  1:192653/1‑149 (MQ=255)
                                                                   tCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGc                                                                    >  2:95935/1‑149 (MQ=255)
                                                                   tCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGc                                                                    >  5:71422/1‑149 (MQ=255)
                                                                             gggTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTa                                                             >  8:119136/1‑146 (MQ=255)
                                                                               gTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGaa                                                        <  7:108785/149‑1 (MQ=255)
                                                                                             aGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGGCACGGCATTCACGG‑CGGCCTGCTTGCCGTCCGCCCAGACGACGCCGATGCAGCCGACGCGGAAGGTTTGGAACTGGGTCAACTTGCGCGGGTAGAGAATGAAACCCTTGGcc                                          <  5:61538/149‑1 (MQ=255)
                                                                                             aGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGcc                                          >  6:72178/1‑149 (MQ=255)
                                                                                             aGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGcc                                          >  3:174369/1‑149 (MQ=255)
                                                                                             aGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGc                                           <  5:351757/148‑1 (MQ=255)
                                                                                                  accAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGa                                      <  7:153008/147‑1 (MQ=255)
                                                                                                    cAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGccc                                                                  >  4:84327/1‑118 (MQ=255)
                                                                                                    cAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGccc                                                                  <  3:84327/118‑1 (MQ=255)
                                                                                                    cAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGAccc                                   <  2:104913/149‑1 (MQ=255)
                                                                                                    cAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGAccc                                   >  8:243159/1‑149 (MQ=255)
                                                                                                     aGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCg                                  >  7:95000/1‑149 (MQ=255)
                                                                                                         cTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCg                              >  2:141790/1‑149 (MQ=255)
                                                                                                           tCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCg                              >  4:275634/1‑147 (MQ=255)
                                                                                                             cATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAg                           <  1:95935/148‑1 (MQ=255)
                                                                                                              aTTTCCCGCAGCACCTCGGCCAAGGCATTCACGG‑CGGCCTGCATGCCGTCAGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATCAAACCCTTGGCCATGATCCGCTCGTac                           >  7:247/1‑146 (MQ=255)
                                                                                                                   ccGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCt                    >  8:165075/1‑149 (MQ=255)
                                                                                                                    cGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCtt                   >  6:324971/1‑149 (MQ=255)
                                                                                                                    cGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCtt                   <  2:192653/149‑1 (MQ=255)
                                                                                                                       agcaCCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGaa                <  1:175717/149‑1 (MQ=255)
                                                                                                                        gcaCCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAAc               <  6:71422/149‑1 (MQ=255)
                                                                                                                        gcaCCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAAc               <  1:216784/149‑1 (MQ=255)
                                                                                                                             tCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTgg            >  3:202555/1‑147 (MQ=255)
                                                                                                                                 ccACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCgg      <  7:243159/149‑1 (MQ=255)
                                                                                                                                 ccACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCgg      <  7:119136/149‑1 (MQ=255)
                                                                                                                                     ggCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCGGGCAt  <  1:141790/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
GGTGCCAGCCCAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCGGGCAT  >  NC_002947/2515330‑2515611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑