Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,515,486 C→A 6.7% G348C (GGC→TGC)  phnW ← 2‑aminoethylphosphonate‑‑pyruvate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,515,4860CA 6.7% 189.4 / 3.7 60G348C (GGC→TGC) phnW2‑aminoethylphosphonate‑‑pyruvate transaminase
Reads supporting (aligned to +/- strand):  ref base C (23/33);  new base A (2/2);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

CCCAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCGGGCATCGGTGGGGGCGTGG  >  NC_002947/2515338‑2515625
                                                                                                                                                     |                                                                                                                                           
cccAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGcc                                                                                                                                             <  8:38063/149‑1 (MQ=255)
 ccAGTCGAGGATGGCGGCTTTCAGTTGGTGGGGGATGTTGTAGATCGTGTGCGCAAACTCCTGAATTCGCGTGGGCAAAGCGTCAGAAATCCAGCACTTCCAATTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCg                                                                                                                                            <  5:397086/149‑1 (MQ=255)
  cAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTTGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGt                                                                                                                                           <  8:272850/149‑1 (MQ=255)
       gAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGGACGGCATGCAGGCCgccgt                                                                                                                                        <  2:302139/148‑5 (MQ=255)
       gAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATACACGG‑CGGCCTGCATGCCGTCCGc                                                                                                                                       <  3:206998/148‑1 (MQ=255)
            tcgcggcTTTCAGTTTGGTGGGGGTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGAc                                                                                                                                 <  8:178931/147‑1 (MQ=255)
            tGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGAc                                                                                                                                 <  6:391575/149‑1 (MQ=255)
                ggcTTGCAGTTGGGTGTGGGTGTTGTAGTTCATGTGCGCTTACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCGACGTCATTCACGG‑CGGCCTGCATGCCGTCCTCCCCGACCAct                                                                                                                             <  2:357917/149‑2 (MQ=255)
                   ttGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGcc                                                                                                                           >  2:175717/1‑148 (MQ=255)
                        gTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCa                                                                                                                     <  8:63949/149‑1 (MQ=255)
                                 ggTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGACGGCATGCAGgccgccgtga                                                                                                                                  >  1:83371/1‑123 (MQ=255)
                                 ggTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGACGGCATGCAGgccgccgtga                                                                                                                                  <  2:83371/127‑5 (MQ=255)
                                  gttgttGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGACGGCATGCAGgccgccgtg                                                                                                                                   >  5:391266/1‑122 (MQ=255)
                                  gttgttGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGACGGCATGCAGgccgccgtg                                                                                                                                   <  6:391266/125‑4 (MQ=255)
                                   ttgttgTAGTTAATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCgg                                                                                                           >  4:71111/1‑148 (MQ=255)
                                        gtAGTTAATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGAACTTCCATTTCCCGCAGCACCTCGGACACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCACCCACCACGCCGATGCAGCCGACGCGGAAGGtt                                                                                                     >  6:61538/1‑149 (MQ=255)
                                           gTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTc                                                                                                   <  4:75213/148‑1 (MQ=255)
                                              cATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGAc                                                                                                <  6:308488/148‑1 (MQ=255)
                                                  tgCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTgg                                                                                            <  1:72052/148‑1 (MQ=255)
                                                  tgCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTgg                                                                                            <  3:71111/148‑1 (MQ=255)
                                                  tgCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTgg                                                                                            <  1:271353/148‑1 (MQ=255)
                                                   gcgcTAACTCCTGAATTCGGGTGGTCAAAGCGTCAGATATCCAGCACTTCCATTTCCCTCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGt                                                                                          <  7:286887/149‑1 (MQ=255)
                                                       tAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAAc                                                                                      >  8:108785/1‑149 (MQ=255)
                                                         aCTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAAc                                                                                      <  1:248110/147‑1 (MQ=255)
                                                         aCTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAAc                                                                                      <  5:392663/147‑1 (MQ=255)
                                                         aCTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACtt                                                                                    <  3:286572/149‑1 (MQ=255)
                                                         aCTCCAGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAAc                                                                                      <  7:271523/147‑1 (MQ=255)
                                                           tCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGc                                                                                  >  1:104913/1‑149 (MQ=255)
                                                           tCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGc                                                                                  >  5:71422/1‑149 (MQ=255)
                                                           tCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGc                                                                                  >  2:95935/1‑149 (MQ=255)
                                                           tCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGc                                                                                  >  1:192653/1‑149 (MQ=255)
                                                                     gggTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTa                                                                           >  8:119136/1‑146 (MQ=255)
                                                                       gTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGaa                                                                      <  7:108785/149‑1 (MQ=255)
                                                                                     aGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGGCACGGCATTCACGG‑CGGCCTGCTTGCCGTCCGCCCAGACGACGCCGATGCAGCCGACGCGGAAGGTTTGGAACTGGGTCAACTTGCGCGGGTAGAGAATGAAACCCTTGGcc                                                        <  5:61538/149‑1 (MQ=255)
                                                                                     aGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGcc                                                        >  6:72178/1‑149 (MQ=255)
                                                                                     aGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGcc                                                        >  3:174369/1‑149 (MQ=255)
                                                                                     aGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGc                                                         <  5:351757/148‑1 (MQ=255)
                                                                                          accAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGa                                                    <  7:153008/147‑1 (MQ=255)
                                                                                            cAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGccc                                                                                >  4:84327/1‑118 (MQ=255)
                                                                                            cAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGccc                                                                                <  3:84327/118‑1 (MQ=255)
                                                                                            cAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGAccc                                                 <  2:104913/149‑1 (MQ=255)
                                                                                            cAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGAccc                                                 >  8:243159/1‑149 (MQ=255)
                                                                                             aGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCg                                                >  7:95000/1‑149 (MQ=255)
                                                                                                 cTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCg                                            >  2:141790/1‑149 (MQ=255)
                                                                                                   tCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCg                                            >  4:275634/1‑147 (MQ=255)
                                                                                                     cATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAg                                         <  1:95935/148‑1 (MQ=255)
                                                                                                      aTTTCCCGCAGCACCTCGGCCAAGGCATTCACGG‑CGGCCTGCATGCCGTCAGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATCAAACCCTTGGCCATGATCCGCTCGTac                                         >  7:247/1‑146 (MQ=255)
                                                                                                           ccGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCt                                  >  8:165075/1‑149 (MQ=255)
                                                                                                            cGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCtt                                 <  2:192653/149‑1 (MQ=255)
                                                                                                            cGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCtt                                 >  6:324971/1‑149 (MQ=255)
                                                                                                               agcaCCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGaa                              <  1:175717/149‑1 (MQ=255)
                                                                                                                gcaCCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAAc                             <  6:71422/149‑1 (MQ=255)
                                                                                                                gcaCCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAAc                             <  1:216784/149‑1 (MQ=255)
                                                                                                                     tCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTgg                          >  3:202555/1‑147 (MQ=255)
                                                                                                                         ccACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCgg                    <  7:119136/149‑1 (MQ=255)
                                                                                                                         ccACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCgg                    <  7:243159/149‑1 (MQ=255)
                                                                                                                             ggCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCGGGCAt                <  1:141790/149‑1 (MQ=255)
                                                                                                                                             cTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCGGGCATCGGTGGGGGCGTgg  >  5:394869/1‑148 (MQ=255)
                                                                                                                                              tGCATGCCGTCCGCCCCGACCACGCCGATGCGGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGCCCCGCTCGTAGAAGTcc                                   >  5:108133/1‑114 (MQ=255)
                                                                                                                                              tGCATGCCGTCCGCCCCGACCACGCCGATGCCGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGCCCCGCTCGTAGAAGTcc                                   <  6:108133/114‑1 (MQ=255)
                                                                                                                                              tGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCGGGCATCGGTGGGGGCGTgg  >  4:287625/1‑147 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
CCCAGTCGAGGATGGCGGCTTGCAGTTGGGTGGGGTTGTTGTAGTTCATGTGCGCTAACTCCTGAATTCGGGTGGGCAAAGCGTCAGATATCCAGCACTTCCATTTCCCGCAGCACCTCGGCCACGGCATTCACGG‑CGGCCTGCATGCCGTCCGCCCCGACCACGCCGATGCAGCCGACGCGGAAGGTTTCGACCTGGGTCAACTTGCCCGGGTAGAGAATGAAACCCTTGGCCTTGACCCGCTCGTAGAAGTCCTTGAACTGGTAGCGGGCATCGGTGGGGGCGTGG  >  NC_002947/2515338‑2515625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑