Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,540,810 G→C 10.4% intergenic (+23/+26) PP_2232 → / ← PP_2233 XRE family transcriptional regulator/isochorismatase family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,540,8100GC10.4% 142.2 / 6.4 48intergenic (+23/+26)PP_2232/PP_2233XRE family transcriptional regulator/isochorismatase family hydrolase
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base C (3/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

TCATCGAAGAGGTCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGCCAGTGG  >  NC_002947/2540663‑2540952
                                                                                                                                                   |                                                                                                                                              
tcatcGAAGAGGTCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCtgt                                                                                                                                               <  1:193854/149‑1 (MQ=255)
   tcGAAGAGGTCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCa                                                                                                                                            <  2:304406/149‑1 (MQ=255)
     gAAGAGGTCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGc                                                                                                                                          <  3:144727/149‑1 (MQ=255)
           gTCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCACAGCAGCCCCaaa                                                                                                                                    <  8:346240/149‑1 (MQ=255)
           gTCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCACAGCAGCCCCa                                                                                                                                      >  7:346240/1‑147 (MQ=255)
           gTCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGGTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCACAGCAGCCCCaaa                                                                                                                                    <  7:185501/149‑1 (MQ=255)
            tCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAg                                                                                                                                   >  2:4739/1‑149 (MQ=255)
                gCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGtct                                                                                                                               <  6:143306/149‑1 (MQ=255)
                   tCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGtctc                                                                                                                              >  8:82435/1‑147 (MQ=255)
                   tCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGtctc                                                                                                                              >  8:159129/1‑147 (MQ=255)
                   tCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCaa                                                                                                                            <  5:269200/149‑1 (MQ=255)
                         gCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGa                                                                                                                       <  3:77954/148‑1 (MQ=255)
                                 ggCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCtttt                                                                                                              <  6:199807/149‑1 (MQ=255)
                                  gCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTc                                                                                                             <  4:261934/149‑1 (MQ=255)
                                      ttCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTcag                                                                                                           >  7:134273/1‑147 (MQ=255)
                                         cATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATTAGCTTTTcatccctg                                                                                                      >  3:85981/1‑143 (MQ=255)
                                              gtTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCtcgtcg                                                                                                 >  7:77382/1‑149 (MQ=255)
                                               ttCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGGTGTTCGGTTTGTGCGCAATGTTGCGATTTGAGGCTGCAGACTTTGGGGGTTCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCtcgtcg                                                                                                 <  7:223783/148‑1 (MQ=255)
                                                             ccGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACAtt                                                                                   <  7:12121/148‑1 (MQ=255)
                                                              cGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTgg                                                                                 <  7:82435/149‑1 (MQ=255)
                                                                gTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCa                                                                               <  3:340229/149‑1 (MQ=255)
                                                                   tGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAg                                                                            <  6:311061/149‑1 (MQ=255)
                                                                          cGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAaacg                                                                     >  1:289186/1‑149 (MQ=255)
                                                                            tAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAATCCGATCAGCTTTTCAGCAGCTCGTCGGTCTACACAACATTTGCATAGGCAaacgc                                                                    >  5:1006/1‑148 (MQ=255)
                                                                              aCGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAaacgccaa                                                                 >  3:36597/1‑149 (MQ=255)
                                                                                        ggtggCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATc                                                       >  7:68189/1‑149 (MQ=255)
                                                                                                ttCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAg                                                                            >  4:35850/1‑120 (MQ=255)
                                                                                                ttCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAg                                                                            <  3:35850/120‑1 (MQ=255)
                                                                                                       tgtgCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGcac                                         <  1:4739/148‑1 (MQ=255)
                                                                                                        gtgCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGcaca                                        <  7:159129/148‑1 (MQ=255)
                                                                                                        gcgcgcAATGTGGCGATTTGAGCCTGCAGAATTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGcaca                                        <  6:204780/146‑1 (MQ=255)
                                                                                                         tgCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATc                                               >  4:142192/1‑140 (MQ=255)
                                                                                                         tgCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATc                                               <  3:142192/140‑1 (MQ=255)
                                                                                                          gcgcAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACAt                                       >  2:197641/1‑147 (MQ=255)
                                                                                                             cAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAgcc                                  >  3:247895/1‑149 (MQ=255)
                                                                                                              aaTGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAgccg                                 >  6:330850/1‑149 (MQ=255)
                                                                                                                tgtgGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAgccgcc                               <  4:236567/149‑1 (MQ=255)
                                                                                                                      gATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCGGGCAcc                         <  2:289186/149‑1 (MQ=255)
                                                                                                                              ccTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGcc                 <  4:247895/149‑1 (MQ=255)
                                                                                                                                tGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGt               >  7:178384/1‑149 (MQ=255)
                                                                                                                                 gCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTc              >  2:184669/1‑149 (MQ=255)
                                                                                                                                     aCTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAAc          <  8:68189/149‑1 (MQ=255)
                                                                                                                                       tttGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGc        >  5:46033/1‑149 (MQ=255)
                                                                                                                                       tttGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGc        >  5:373803/1‑149 (MQ=255)
                                                                                                                                       tttGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCAAAACATTTGCATAGGCAAACGCCAACGCCGCCATAGCCGAATAATTCAAATGAGCCGCCGGCACCTGCTTGCCGGCGAACGc        >  1:318990/1‑149 (MQ=255)
                                                                                                                                       tttGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCCGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGc        >  5:384563/1‑149 (MQ=255)
                                                                                                                                       tttGGGGCTGCACAGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGc        >  3:237623/1‑149 (MQ=255)
                                                                                                                                       tttGGGGCTGCACAGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGc        >  1:205419/1‑149 (MQ=255)
                                                                                                                                             gctgctGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGCCAGTgg  >  2:41748/1‑149 (MQ=255)
                                                                                                                                              ctgctgTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCCTCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGCCAGTgg  >  7:64503/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TCATCGAAGAGGTCGAGCATCGCTTGCAGGTGGGGCAGTTCCATGTGTTCCCCAGTAACTGCCGGTATGCCTACCGTAACGATGGCGTGGTGGCTGTTCGGTTTGTGCGCAATGTGGCGATTTGAGCCTGCAGACTTTGGGGCTGCTGTGCAGCCCCAAAGGTCTCAAGCCGATCAGCTTTTCAGCAGCTCGTCGGTCTTCACCACATTGGCATAGGCAAACGCCAACGCCGCCATCGCCGAATCATGCACATGAGCCGCCGGCACCTGCTTGCCGTCGAACGCCAGTGG  >  NC_002947/2540663‑2540952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑