Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,746,707 A→G 7.8% S354S (AGT→AGC)  PP_2402 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,746,7070AG 7.8% 143.3 / 3.1 51S354S (AGT→AGC) PP_2402sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (26/21);  new base G (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTCGACCTGGGCGCCGCT  >  NC_002947/2746559‑2746853
                                                                                                                                                    |                                                                                                                                                  
tCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTa                                                                                                                                                    >  5:119862/1‑149 (MQ=255)
tCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTa                                                                                                                                                    <  8:176563/149‑1 (MQ=255)
       gtggGCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGccc                                                                                                                                              <  6:256424/148‑1 (MQ=255)
         gggCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACt                                                                                                                                                  <  6:193125/142‑1 (MQ=255)
         gggCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACt                                                                                                                                                  >  5:193125/1‑142 (MQ=255)
            cAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTGCTGCCCTcgcgc                                                                                                                                        >  4:91096/1‑149 (MQ=255)
            cAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTGCTGCCCTcgcgc                                                                                                                                        >  4:85770/1‑149 (MQ=255)
              aTGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTGCTGCCCTCGCGCGa                                                                                                                                      <  3:91096/149‑1 (MQ=255)
              aTGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTGCTGCCCTCGCGCGa                                                                                                                                      <  3:85770/149‑1 (MQ=255)
                 gCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGacac                                                                                                                                   <  2:71909/149‑1 (MQ=255)
                  ccAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACt                                                                                                                                  >  8:20830/1‑149 (MQ=255)
                        ccAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGc                                                                                                                            >  7:40853/1‑149 (MQ=255)
                                    cACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCt                                                                                                                >  5:342109/1‑149 (MQ=255)
                                     aCCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTg                                                                                                               >  2:18312/1‑149 (MQ=255)
                                      cccGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTg                                                                                                               >  5:154400/1‑148 (MQ=255)
                                      cccGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGc                                                                                                              <  5:62845/149‑1 (MQ=255)
                                             tgcGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACtt                                                                                                        >  5:366269/1‑148 (MQ=255)
                                               cGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCa                                                                                                      <  6:342109/148‑1 (MQ=255)
                                                gATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAgg                                                                                                    <  7:7493/149‑1 (MQ=255)
                                                 aTTGCGTGAACCCTCCACCCGGTAGAACGGCGTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAggg                                                                                                   <  4:19659/149‑1 (MQ=255)
                                                     cGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGtt                                                                                               >  5:155934/1‑149 (MQ=255)
                                                      gTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCGCGTTGTAGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTg                                                                                              >  5:300531/1‑149 (MQ=255)
                                                       tGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGt                                                                                             >  2:136574/1‑149 (MQ=255)
                                                         aaCCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCg                                                                                           >  3:119955/1‑149 (MQ=255)
                                                           cccTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGAc                                                                                         <  7:20830/149‑1 (MQ=255)
                                                                      gTAGAACGGCTTGAGGACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCa                                                                              <  4:246294/149‑1 (MQ=255)
                                                                                            tCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATgcgg                                                        >  2:353947/1‑149 (MQ=255)
                                                                                              cAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATgcgggc                                                      <  6:200207/149‑1 (MQ=255)
                                                                                               aGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGTATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATgcgggcg                                                     <  5:386873/149‑1 (MQ=255)
                                                                                                gTTCGTCAACGGGTATCCCTTGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTTCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATgcgggcgg                                                    <  6:300531/149‑1 (MQ=255)
                                                                                                gTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATgcgggcgg                                                    <  1:353947/149‑1 (MQ=255)
                                                                                                gTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACAAGGTTCACCAGTACCCGGCGCAGCGCATgcgggcgg                                                    >  6:41855/1‑149 (MQ=255)
                                                                                                gTTCGTCAACGGGTATCCCTGGCCCGTTCTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATgcgggcgg                                                    >  1:220936/1‑149 (MQ=255)
                                                                                                            gTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAgg                                        <  1:18312/149‑1 (MQ=255)
                                                                                                                  cTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCg                                  >  4:42221/1‑149 (MQ=255)
                                                                                                                   tGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCgc                                 >  6:116759/1‑149 (MQ=255)
                                                                                                                   tGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGCTTCACCAGTACCCGGCGCACCGCATGCCGGCGGGTTTCCAGCAGGGTATCgc                                 >  5:130097/1‑149 (MQ=255)
                                                                                                                     gCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCgct                                <  5:116759/148‑1 (MQ=255)
                                                                                                                       ccGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCgctgct                             >  6:324040/1‑149 (MQ=255)
                                                                                                                        cGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGctgatg                            <  6:85408/149‑1 (MQ=255)
                                                                                                                             tCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTg                       >  8:285704/1‑149 (MQ=255)
                                                                                                                               gAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTTGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTgg                      <  8:40853/148‑1 (MQ=255)
                                                                                                                               gAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGgc                     >  4:333684/1‑149 (MQ=255)
                                                                                                                               gAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGgc                     <  7:285704/149‑1 (MQ=255)
                                                                                                                                 gCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGgcgc                   >  8:314744/1‑149 (MQ=255)
                                                                                                                                   aCGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTc                 >  4:154916/1‑149 (MQ=255)
                                                                                                                                   aCGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTc                 >  6:6833/1‑149 (MQ=255)
                                                                                                                                    cgcgGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTCg                >  7:275951/1‑149 (MQ=255)
                                                                                                                                     gcgGATGATGGAGGTACTGCCCTCGCGCGACACTTGCAGCTCGCCAGCACCTGCAAACTTGAGGGCGTTCTCCACCACGTTCACCAGTACCCGGCGCAGCGCATCCCGCCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTCGa               >  3:192225/1‑149 (MQ=255)
                                                                                                                                      cgGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTCGAc              <  6:155934/149‑1 (MQ=255)
                                                                                                                                               gtggtACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTCGACCTGGgcgc      <  3:42221/148‑1 (MQ=255)
                                                                                                                                                tggtACTGCCAACGAGCGACAATTGCAGCTCGGCACCACCTGCAAACTGCAGGGCGTTGTCGACCAGGTGCACACCTTCCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTCGACCTGGgcggcg    >  6:141815/1‑149 (MQ=255)
                                                                                                                                                  gtACTGCCCTCGGGCGACACTAGCAGCTCGGCATCACCTGCAAACTTGAGGGCGTTGGCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCTCTGCTGCCGTGGCGCTCGTCCTGGGCGCCGCt  <  5:141815/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGATTGCGTGAACCCTCCACCCGGTAGAACGGCTTGAGCACTTCATCCAGTTCGTCAACGGGTATCCCTGGCCCGTTGTCGAGCACGCGGATGATGGTGGTACTGCCCTCGCGCGACACTTGCAGCTCGGCAGCACCTGCAAACTTCAGGGCGTTGTCGACCAGGTTCACCAGTACCCGGCGCAGCGCATGCGGGCGGGTTTCCAGCAGGGTATCGCTGCTGCCGTGGCGCTCGACCTGGGCGCCGCT  >  NC_002947/2746559‑2746853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑