Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,776,107	A→C	5.7%	K152Q (AAG→CAG)	sotB →	sugar efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,776,107	0	A	C	5.7%	223.7 / 2.4	77	K152Q (AAG→CAG)	sotB	sugar efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (34/38);  new base C (3/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGAT  >  NC_002947/2775963‑2776252                                                                                                                                                    |                                                                                                                                                    aTTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCCAgcg                                                                                                                                                 >  3:250569/1‑147 (MQ=255) aTTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGgc                                                                                                                                                 <  7:275374/149‑1 (MQ=255) aTTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGgc                                                                                                                                                 >  6:299209/1‑149 (MQ=255)       aGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑Tgg                                                                                                                                           <  6:127128/148‑1 (MQ=255)         ccACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCC‑‑‑GCCCGCCAAGGCGC‑Tggggc                                                                                                                                        >  2:292535/1‑147 (MQ=255)           aCTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctg                                                                                                                                      <  1:192160/149‑1 (MQ=255)            cTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctgc                                                                                                                                     <  6:249164/149‑1 (MQ=255)               gCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCCAGCGCC‑TTGGCctgctg                                                                                                                                   <  4:250569/148‑1 (MQ=255)               gCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctgctg                                                                                                                                   <  1:382778/148‑1 (MQ=255)                   tCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCCGGCCAAGGCGC‑TGGGCCTGCAGGcccc                                                                                                                              >  4:248930/1‑147 (MQ=255)                       ggCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑gg                                                                                                                          >  8:255741/1‑148 (MQ=255)                        gCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGt                                                                                                                         <  3:55618/148‑1 (MQ=255)                              gCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTAccc                                                                                                                  >  8:234179/1‑149 (MQ=255)                              gCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTAccc                                                                                                                  >  3:282877/1‑149 (MQ=255)                              gCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTAcc                                                                                                                   >  4:42319/1‑148 (MQ=255)                                  aGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑CCAGGCCAAGGCGG‑GGGGCCTGCGGGCCCCC‑CCTACTACCCAGGc                                                                                                              >  1:144689/1‑149 (MQ=255)                                   gCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGcc                                                                                                             <  8:335056/149‑1 (MQ=255)                                    cTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCa                                                                                                            <  2:222189/149‑1 (MQ=255)                                     ttCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctgc                                                                                                                                     <  2:306864/124‑1 (MQ=255)                                     ttCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctgc                                                                                                                                     >  1:306864/1‑124 (MQ=255)                                       cGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATgg                                                                                                         >  2:276093/1‑149 (MQ=255)                                       cGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATgg                                                                                                         >  3:65662/1‑149 (MQ=255)                                          cATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCaa                                                                                                                                                    <  8:3598/105‑1 (MQ=255)                                          cATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCaa                                                                                                                                                    >  7:3598/1‑105 (MQ=255)                                           aTGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑ACAGGCCAACTCGC‑TGGGCCAGCAGGCCACC‑CGTACTACCCTAGACAAGGTc                                                                                                       >  8:351978/1‑147 (MQ=255)                                            tgctgcTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTg                                                                                                    <  8:231238/149‑1 (MQ=255)                                             gctgctGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGGCTGCTGGCCACC‑GGGACTAACCTGGCCAGGGTCCTgg                                                                                                   >  6:205194/1‑149 (MQ=255)                                              ctgctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGTCCATGGTCCTgg                                                                                                   <  8:326896/148‑1 (MQ=255)                                              ctgctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTgg                                                                                                   <  2:350869/148‑1 (MQ=255)                                              ctgctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTgg                                                                                                   <  3:302063/148‑1 (MQ=255)                                               tgctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTggg                                                                                                  >  6:355109/1‑148 (MQ=255)                                                    gTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGtactac                                                                                                                    >  6:322157/1‑125 (MQ=255)                                                    gTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGtactac                                                                                                                    <  5:322157/125‑1 (MQ=255)                                                       aGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCt                                                                                         >  4:135578/1‑149 (MQ=255)                                                        gCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTg                                                                                        <  7:47857/149‑1 (MQ=255)                                                           gCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCTTCCCGCTGGGc                                                                                     >  2:34617/1‑149 (MQ=255)                                                                  cATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAAAGGCAGGCCAAAGCGCGAGGGCCTGCTGGCCACC‑GGTACTAACCTGGCCATGGTCCTGGGCATCCCGCTGGGCCgt                                                                                  >  1:122126/1‑149 (MQ=255)                                                                   aTTGCCCTGGCGCATGCGGTGTTCATGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑CCATGCCAAGGCGC‑AGGGGCTGCTGGCCACC‑CGTACTAACCAGGCCAAGGAACATGGCAAACCGCAGGGACgtgtt                                                                               >  6:211495/1‑146 (MQ=255)                                                                      gCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCTTCCCGCTGGGCCGTGtggtgg                                                                           <  1:34617/148‑1 (MQ=255)                                                                       cccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACGACCCAGGCCATGGTCCGGGGCCTCCCGCTGGGCCGGGTGGTGGGc                                                                         >  8:349760/1‑149 (MQ=255)                                                                       cccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGCCCTGCTGGCCCCC‑GCGACAACCCTCGCCCTGGTCCTGGGCATACCGCAGGGCCCTCtggtggcc                                                                         >  3:288052/1‑147 (MQ=255)                                                                         cTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGa                                                                       <  7:351978/149‑1 (MQ=255)                                                                         cTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGa                                                                       <  3:135578/149‑1 (MQ=255)                                                                            gcgcATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGta                                                                                                                        <  3:70002/97‑1 (MQ=255)                                                                            gcgcATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGta                                                                                                                        >  4:70002/1‑97 (MQ=255)                                                                            gcgcATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAg                                                                      <  3:42319/147‑1 (MQ=255)                                                                                aTGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTGCTGGGCATACCGCTGGGCCGTGTGGTGGGCGCGGCACTg                                                                >  3:182473/1‑149 (MQ=255)                                                                                    ggTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCt                                                                                         <  5:235522/120‑1 (MQ=255)                                                                                             gTCCATCTCCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTa                                                      <  1:133916/146‑1 (MQ=255)                                                                                             gTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCGGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCa                                                   >  4:357946/1‑149 (MQ=255)                                                                                                cATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCCCGt                                                >  5:277594/1‑149 (MQ=255)                                                                                                cATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGt                                                <  4:65662/149‑1 (MQ=255)                                                                                                 aTCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTggg                                                              <  3:93371/134‑1 (MQ=255)                                                                                                 aTCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGtt                                               >  5:361612/1‑149 (MQ=255)                                                                                                  tCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTc                                              <  4:282877/149‑1 (MQ=255)                                                                                                    aCCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCt                                            <  5:355109/149‑1 (MQ=255)                                                                                                      cGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGa                                          <  2:174271/149‑1 (MQ=255)                                                                                                      cGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGa                                          <  7:349760/149‑1 (MQ=255)                                                                                                        ccTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGc                                                         <  5:120104/132‑1 (MQ=255)                                                                                                            gCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCAtt                                     <  4:244952/148‑1 (MQ=255)                                                                                                                gCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCgg                                <  2:122126/149‑1 (MQ=255)                                                                                                                        tgtgGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAgcgc                        <  3:248930/149‑1 (MQ=255)                                                                                                                            gcgcCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACCGGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAg                     >  1:86055/1‑149 (MQ=255)                                                                                                                            gcgcCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAg                     <  2:86055/148‑1 (MQ=255)                                                                                                                              gccgccGGGCCA‑‑‑GCAGGCCAACCCGC‑GGGGCCGGCTGGCCCCC‑CGTACGACCCTGGCCCAGGGCCGGGGGCTCCCCCGGGGGCGTGTGGTGGGCGCGGCACGGGGCGGGCGTTTCACGTTGCGGCGCATTGCCGGGGTAGGGGTAGccc                  >  6:6162/1‑148 (MQ=255)                                                                                                                                cgccgGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGccacc                <  7:255741/149‑1 (MQ=255)                                                                                                                                     ggCCA‑‑‑GCAGGCCCAGCGCC‑TTGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGGGGCACTGGGCTGGCGTATCACGTTCCt                                            <  2:175897/116‑1 (MQ=38)                                                                                                                                     ggCCA‑‑‑GCAGGCCCAGCGCC‑TTGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGGGGCACTGGGCTGGCGTATCACGTTCCt                                            >  1:175897/1‑116 (MQ=38)                                                                                                                                        ca‑‑‑gcagGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGGATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTg        >  6:259302/1‑149 (MQ=255)                                                                                                                                         a‑‑‑gcagGCCCAGCGCC‑TTGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGc       >  2:26696/1‑149 (MQ=255)                                                                                                                                         a‑‑‑gcagGCCAAGGCGC‑TGGGCCGGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATGACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGc       <  1:276093/149‑1 (MQ=255)                                                                                                                                             gcagGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCCTCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGcc      >  7:143893/1‑149 (MQ=255)                                                                                                                                             gcagGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGcc      <  5:205194/149‑1 (MQ=255)                                                                                                                                                 gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGc                                                         >  3:151096/1‑94 (MQ=255)                                                                                                                                                 gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGc                                                         <  4:151096/94‑1 (MQ=255)                                                                                                                                                 gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTCGCGCTAGCCACCATGC‑TTGCCTGAt  >  8:155987/1‑148 (MQ=255)                                                                                                                                                 gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGAt  >  7:356142/1‑149 (MQ=255)                                                                                                                                                 gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGAt  <  7:234179/149‑1 (MQ=255)                                                                                                                                                  ccAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGAt  >  4:340539/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGAT  >  NC_002947/2775963‑2776252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑