Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,776,112 G→C 5.4% A153A (GCG→GCC) ‡ sotB → sugar efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,776,1120GC 5.4% 233.9 / 3.8 74A153A (GCG→GCC) ‡sotBsugar efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (33/37);  new base C (2/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01

AGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCG  >  NC_002947/2775969‑2776259
                                                                                                                                                  |                                                                                                                                                     
aGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑Tgg                                                                                                                                                  <  6:127128/148‑1 (MQ=255)
  ccACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCC‑‑‑GCCCGCCAAGGCGC‑Tggggc                                                                                                                                               >  2:292535/1‑147 (MQ=255)
    aCTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctg                                                                                                                                             <  1:192160/149‑1 (MQ=255)
     cTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctgc                                                                                                                                            <  6:249164/149‑1 (MQ=255)
        gCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCCAGCGCC‑TTGGCctgctg                                                                                                                                          <  4:250569/148‑1 (MQ=255)
        gCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctgctg                                                                                                                                          <  1:382778/148‑1 (MQ=255)
            tCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCCGGCCAAGGCGC‑TGGGCCTGCAGGcccc                                                                                                                                     >  4:248930/1‑147 (MQ=255)
                ggCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑gg                                                                                                                                 >  8:255741/1‑148 (MQ=255)
                 gCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGt                                                                                                                                <  3:55618/148‑1 (MQ=255)
                       gCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTAccc                                                                                                                         >  3:282877/1‑149 (MQ=255)
                       gCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTAccc                                                                                                                         >  8:234179/1‑149 (MQ=255)
                       gCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTAcc                                                                                                                          >  4:42319/1‑148 (MQ=255)
                           aGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑CCAGGCCAAGGCGG‑GGGGCCTGCGGGCCCCC‑CCTACTACCCAGGc                                                                                                                     >  1:144689/1‑149 (MQ=255)
                            gCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGcc                                                                                                                    <  8:335056/149‑1 (MQ=255)
                             cTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCa                                                                                                                   <  2:222189/149‑1 (MQ=255)
                              ttCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctgc                                                                                                                                            >  1:306864/1‑124 (MQ=255)
                              ttCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCctgc                                                                                                                                            <  2:306864/124‑1 (MQ=255)
                                cGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATgg                                                                                                                >  3:65662/1‑149 (MQ=255)
                                cGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATgg                                                                                                                >  2:276093/1‑149 (MQ=255)
                                    aTGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑ACAGGCCAACTCGC‑TGGGCCAGCAGGCCACC‑CGTACTACCCTAGACAAGGTc                                                                                                              >  8:351978/1‑147 (MQ=255)
                                     tgctgcTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTg                                                                                                           <  8:231238/149‑1 (MQ=255)
                                      gctgctGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGGCTGCTGGCCACC‑GGGACTAACCTGGCCAGGGTCCTgg                                                                                                          >  6:205194/1‑149 (MQ=255)
                                       ctgctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGTCCATGGTCCTgg                                                                                                          <  8:326896/148‑1 (MQ=255)
                                       ctgctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTgg                                                                                                          <  2:350869/148‑1 (MQ=255)
                                       ctgctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTgg                                                                                                          <  3:302063/148‑1 (MQ=255)
                                        tgctgGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTggg                                                                                                         >  6:355109/1‑148 (MQ=255)
                                             gTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGtactac                                                                                                                           <  5:322157/125‑1 (MQ=255)
                                             gTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGtactac                                                                                                                           >  6:322157/1‑125 (MQ=255)
                                                aGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCt                                                                                                >  4:135578/1‑149 (MQ=255)
                                                 gCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTg                                                                                               <  7:47857/149‑1 (MQ=255)
                                                    gCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCTTCCCGCTGGGc                                                                                            >  2:34617/1‑149 (MQ=255)
                                                           cATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAAAGGCAGGCCAAAGCGCGAGGGCCTGCTGGCCACC‑GGTACTAACCTGGCCATGGTCCTGGGCATCCCGCTGGGCCgt                                                                                         >  1:122126/1‑149 (MQ=255)
                                                            aTTGCCCTGGCGCATGCGGTGTTCATGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑CCATGCCAAGGCGC‑AGGGGCTGCTGGCCACC‑CGTACTAACCAGGCCAAGGAACATGGCAAACCGCAGGGACgtgtt                                                                                      >  6:211495/1‑146 (MQ=255)
                                                               gCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCTTCCCGCTGGGCCGTGtggtgg                                                                                  <  1:34617/148‑1 (MQ=255)
                                                                cccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACGACCCAGGCCATGGTCCGGGGCCTCCCGCTGGGCCGGGTGGTGGGc                                                                                >  8:349760/1‑149 (MQ=255)
                                                                cccTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGCCCTGCTGGCCCCC‑GCGACAACCCTCGCCCTGGTCCTGGGCATACCGCAGGGCCCTCtggtggcc                                                                                >  3:288052/1‑147 (MQ=255)
                                                                  cTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGa                                                                              <  7:351978/149‑1 (MQ=255)
                                                                  cTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGa                                                                              <  3:135578/149‑1 (MQ=255)
                                                                     gcgcATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGta                                                                                                                               <  3:70002/97‑1 (MQ=255)
                                                                     gcgcATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGta                                                                                                                               >  4:70002/1‑97 (MQ=255)
                                                                     gcgcATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAg                                                                             <  3:42319/147‑1 (MQ=255)
                                                                         aTGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTGCTGGGCATACCGCTGGGCCGTGTGGTGGGCGCGGCACTg                                                                       >  3:182473/1‑149 (MQ=255)
                                                                             ggTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCt                                                                                                <  5:235522/120‑1 (MQ=255)
                                                                                      gTCCATCTCCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTa                                                             <  1:133916/146‑1 (MQ=255)
                                                                                      gTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCGGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCa                                                          >  4:357946/1‑149 (MQ=255)
                                                                                         cATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCCCGt                                                       >  5:277594/1‑149 (MQ=255)
                                                                                         cATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGt                                                       <  4:65662/149‑1 (MQ=255)
                                                                                          aTCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTggg                                                                     <  3:93371/134‑1 (MQ=255)
                                                                                          aTCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGtt                                                      >  5:361612/1‑149 (MQ=255)
                                                                                           tCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTc                                                     <  4:282877/149‑1 (MQ=255)
                                                                                             aCCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCt                                                   <  5:355109/149‑1 (MQ=255)
                                                                                               cGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGa                                                 <  7:349760/149‑1 (MQ=255)
                                                                                               cGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGa                                                 <  2:174271/149‑1 (MQ=255)
                                                                                                 ccTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGc                                                                <  5:120104/132‑1 (MQ=255)
                                                                                                     gCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCAtt                                            <  4:244952/148‑1 (MQ=255)
                                                                                                         gCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCgg                                       <  2:122126/149‑1 (MQ=255)
                                                                                                                 tgtgGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAgcgc                               <  3:248930/149‑1 (MQ=255)
                                                                                                                     gcgcCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACCGGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAg                            >  1:86055/1‑149 (MQ=255)
                                                                                                                     gcgcCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAg                            <  2:86055/148‑1 (MQ=255)
                                                                                                                       gccgccGGGCCA‑‑‑GCAGGCCAACCCGC‑GGGGCCGGCTGGCCCCC‑CGTACGACCCTGGCCCAGGGCCGGGGGCTCCCCCGGGGGCGTGTGGTGGGCGCGGCACGGGGCGGGCGTTTCACGTTGCGGCGCATTGCCGGGGTAGGGGTAGccc                         >  6:6162/1‑148 (MQ=255)
                                                                                                                         cgccgGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGccacc                       <  7:255741/149‑1 (MQ=255)
                                                                                                                              ggCCA‑‑‑GCAGGCCCAGCGCC‑TTGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGGGGCACTGGGCTGGCGTATCACGTTCCt                                                   <  2:175897/116‑1 (MQ=38)
                                                                                                                              ggCCA‑‑‑GCAGGCCCAGCGCC‑TTGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGGGGCACTGGGCTGGCGTATCACGTTCCt                                                   >  1:175897/1‑116 (MQ=38)
                                                                                                                                 ca‑‑‑gcagGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGGATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTg               >  6:259302/1‑149 (MQ=255)
                                                                                                                                  a‑‑‑gcagGCCCAGCGCC‑TTGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGc              >  2:26696/1‑149 (MQ=255)
                                                                                                                                  a‑‑‑gcagGCCAAGGCGC‑TGGGCCGGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATGACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGc              <  1:276093/149‑1 (MQ=255)
                                                                                                                                      gcagGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCCTCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGcc             >  7:143893/1‑149 (MQ=255)
                                                                                                                                      gcagGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGcc             <  5:205194/149‑1 (MQ=255)
                                                                                                                                          gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGc                                                                <  4:151096/94‑1 (MQ=255)
                                                                                                                                          gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGc                                                                >  3:151096/1‑94 (MQ=255)
                                                                                                                                          gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTCGCGCTAGCCACCATGC‑TTGCCTGAt         >  8:155987/1‑148 (MQ=255)
                                                                                                                                          gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGAt         >  7:356142/1‑149 (MQ=255)
                                                                                                                                          gCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGAt         <  7:234179/149‑1 (MQ=255)
                                                                                                                                           ccAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGAt         >  4:340539/1‑148 (MQ=255)
                                                                                                                                                 cgc‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATcg  <  5:299209/149‑1 (MQ=255)
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AGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCA‑‑‑GCAGGCCAAGGCGC‑TGGGCCTGCTGGCCACC‑GGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCG  >  NC_002947/2775969‑2776259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑