Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,787,623 G→A 9.8% intergenic (+70/‑55) ahpC → / → ahpF peroxiredoxin/alkylhydroperoxide reductase small subunit/alkyl hydroperoxide reductase subunit F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,787,6230GA 9.8% 136.4 / 4.8 51intergenic (+70/‑55)ahpC/ahpFperoxiredoxin/alkylhydroperoxide reductase small subunit/alkyl hydroperoxide reductase subunit F
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base A (2/3);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01

AGGCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGC  >  NC_002947/2787475‑2787769
                                                                                                                                                     |                                                                                                                                                  
aGGCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCg                                                                                                                                                    >  4:19473/1‑149 (MQ=255)
                 ccAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGc                                                                                                                                   <  1:345325/149‑1 (MQ=255)
                 ccAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGc                                                                                                                                   <  4:362033/149‑1 (MQ=255)
                     gTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGCGttt                                                                                                                               <  2:70908/149‑1 (MQ=255)
                        gAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGttttt                                                                                                                             <  5:194495/148‑1 (MQ=255)
                        gAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTg                                                                                                                            >  1:346696/1‑149 (MQ=255)
                          aagaagGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTa                                                                                                                          >  3:11397/1‑149 (MQ=255)
                                              gcgcCGTCGCTGGACCTCGTAGGCAAGATCTTAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGaaaaa                                                                                                          <  7:34031/145‑1 (MQ=255)
                                                  cGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGc                                                                                                  <  4:157316/149‑1 (MQ=255)
                                                    tCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGccc                                                                                                <  3:311870/149‑1 (MQ=255)
                                                     cGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGGCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCg                                                                                               <  7:151244/149‑1 (MQ=255)
                                                                  aGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGc                                                                                   <  6:362947/148‑1 (MQ=255)
                                                                   ggCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCa                                                                                 >  5:388195/1‑149 (MQ=255)
                                                                           cTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTaaa                                                                         >  6:19614/1‑149 (MQ=255)
                                                                            tAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAAt                                                                        >  7:309/1‑149 (MQ=255)
                                                                             aaGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATc                                                                       <  4:19773/149‑1 (MQ=255)
                                                                              aGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAAt                                                                        <  3:19473/147‑1 (MQ=255)
                                                                              aGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATc                                                                       <  7:358401/148‑1 (MQ=255)
                                                                              aGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCg                                                                      <  2:346696/149‑1 (MQ=255)
                                                                               ggTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCg                                                                      >  2:20956/1‑148 (MQ=255)
                                                                               ggTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGc                                                                     <  6:388195/149‑1 (MQ=255)
                                                                               ggTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGc                                                                     <  5:117419/149‑1 (MQ=255)
                                                                                   aTGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACt                                                                 <  5:43410/149‑1 (MQ=255)
                                                                                     gCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGATAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCGTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATAGCCCGTATGTTGGACg                                                                                    >  1:267311/1‑129 (MQ=255)
                                                                                     gCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTg                                                                >  4:221543/1‑148 (MQ=255)
                                                                                        cATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGaaaa                                                            >  7:13520/1‑149 (MQ=255)
                                                                                        cATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGaaaa                                                            >  7:275574/1‑149 (MQ=255)
                                                                                          tAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAAcc                                                          <  7:221181/149‑1 (MQ=255)
                                                                                          tAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAAcc                                                          <  7:215422/149‑1 (MQ=255)
                                                                                           aaCGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAACAcc                                                          >  8:97718/1‑148 (MQ=255)
                                                                                                      tAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCgg                                              <  8:275574/149‑1 (MQ=255)
                                                                                                      tAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCgg                                              >  2:337253/1‑149 (MQ=255)
                                                                                                        agcagcCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCggg                                             <  3:221543/148‑1 (MQ=255)
                                                                                                            gccgccAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCAcc                                        <  4:11397/149‑1 (MQ=255)
                                                                                                                ccAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGc                                    >  8:304405/1‑149 (MQ=255)
                                                                                                                     gCTGT‑ATTACCTTCCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATc                               <  7:204331/149‑1 (MQ=255)
                                                                                                                     gCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTgg                                                   >  4:176365/1‑129 (MQ=255)
                                                                                                                     gCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTgg                                                   <  3:176365/129‑1 (MQ=255)
                                                                                                                       tGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATc                               >  6:249622/1‑147 (MQ=255)
                                                                                                                              acctaccGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTGAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGt                       >  6:13788/1‑149 (MQ=255)
                                                                                                                               cctaccGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGtt                      <  8:309/149‑1 (MQ=255)
                                                                                                                               cctaccGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGtt                      <  1:337253/149‑1 (MQ=255)
                                                                                                                                ctaccGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCAAGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTg                     <  8:13520/149‑1 (MQ=255)
                                                                                                                                    cGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTc                 >  8:106481/1‑149 (MQ=255)
                                                                                                                                         cGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTc             >  2:69466/1‑148 (MQ=255)
                                                                                                                                           aaaaaCGCCCAGGCGAGGTCGCCCGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTcgac          >  3:65145/3‑149 (MQ=255)
                                                                                                                                              aaCGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTccc               >  6:233131/1‑141 (MQ=255)
                                                                                                                                              aaCGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTccc               <  5:233131/141‑1 (MQ=255)
                                                                                                                                              aaCGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCGACTTGGAGCGGGTCACCCCGCCGATAGAGATCGTTGCTTCCCTCgacgac       >  5:208621/1‑149 (MQ=255)
                                                                                                                                                 gCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCAGAGTTTTGAAAAAGAAATCTACAGAATGTTGGAAGCCAAGCTTAAATAGCAACTGAAAAAATACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGgc    >  6:162264/1‑149 (MQ=255)
                                                                                                                                                 gCCCAGGCGAGGTCGCCCGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGgc    >  7:167489/1‑149 (MQ=255)
                                                                                                                                                   ccGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGgcgc  >  2:177123/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGGCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGA‑AGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGC  >  NC_002947/2787475‑2787769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑