Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,804,385 G→C 7.3% V66V (GTG→GTC)  rbsR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,804,3850GC 7.3% 209.4 / 4.6 65V66V (GTG→GTC) rbsRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base C (2/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAA  >  NC_002947/2804244‑2804526
                                                                                                                                             |                                                                                                                                             
ccATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCaa                                                                                                                                          >  3:28023/1‑147 (MQ=255)
 cATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAg                                                                                                                                       >  5:383044/1‑149 (MQ=255)
  aTGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCTAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGc                                                                                                                                      >  5:396813/1‑149 (MQ=255)
   tGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGGCACCAACAggc                                                                                                                                     <  2:134356/149‑3 (MQ=255)
   tGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCGCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGGCACCAACAggc                                                                                                                                     <  6:361904/149‑3 (MQ=255)
     tACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTc                                                                                                                                   <  4:28023/149‑1 (MQ=255)
      aCTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTc                                                                                                                                   >  3:71460/1‑148 (MQ=255)
        tCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAAc                                                                                                                                >  6:398322/1‑149 (MQ=255)
        tCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAAc                                                                                                                                >  5:247618/1‑149 (MQ=255)
          aacaaGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAAccc                                                                                                                              <  6:154134/149‑1 (MQ=255)
            caaGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCg                                                                                                                             >  2:67861/1‑148 (MQ=255)
             aaGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTa                                                                                                                           <  6:315551/149‑1 (MQ=255)
                  ccGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTc                                                                                                                       <  3:65064/148‑1 (MQ=255)
                  ccGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTc                                                                                                                       >  4:65064/1‑148 (MQ=255)
                   cGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGc                                                                                                                     <  3:120023/149‑1 (MQ=255)
                    gTCCGGTCGGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGcc                                                                                                                    >  2:162798/1‑149 (MQ=255)
                     tCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACa                                                                                                                                        >  7:358866/1‑128 (MQ=255)
                     tCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACa                                                                                                                                        <  8:358866/128‑1 (MQ=255)
                       cGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGa                                                                                                                  <  3:196479/148‑1 (MQ=255)
                        ggTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGc                                                                                                                <  2:99905/149‑1 (MQ=255)
                        ggTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGc                                                                                                                <  2:105165/149‑1 (MQ=255)
                         gTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCt                                                                                                               <  2:276753/149‑1 (MQ=255)
                            aGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTgg                                                                                                             <  4:302088/148‑1 (MQ=255)
                            aGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGgc                                                                                                            >  8:337387/1‑149 (MQ=255)
                                 gCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCggg                                                                                                       <  1:162798/149‑1 (MQ=255)
                                  cAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCgggg                                                                                                      >  8:73080/1‑149 (MQ=255)
                                   aGGTGCGGCAGAAAGTGGCAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGt                                                                                                     <  7:148037/149‑1 (MQ=255)
                                     gTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTCCTTCGCCGCGCTGGCGCGGGGTAt                                                                                                   >  6:322948/1‑149 (MQ=255)
                                     gTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCgg                                                                                                        <  8:149041/144‑1 (MQ=255)
                                     gTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCgg                                                                                                        >  7:149041/1‑144 (MQ=255)
                                     gTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTAt                                                                                                   >  1:287487/1‑149 (MQ=255)
                                         ggCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGaa                                                                                               <  5:398322/149‑1 (MQ=255)
                                          gCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGGCACCAACAggc                                                                                                                                     <  4:204074/110‑3 (MQ=255)
                                          gCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGGCACCAACAggc                                                                                                                                     >  3:204074/1‑108 (MQ=255)
                                           cTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGa                                                                                             <  2:148386/149‑1 (MQ=255)
                                                  tGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGc                                                                                      <  4:71460/149‑1 (MQ=255)
                                                    gAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGc                                                                                      >  4:176524/1‑147 (MQ=255)
                                                          gccATCATCCAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGAACGGCCACCATCGGCCTGTTGGTCCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCCAGCGCaa                                                                              >  2:92560/1‑149 (MQ=255)
                                                                   gAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGTCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTAtt                                                                      <  6:312319/148‑1 (MQ=255)
                                                                         gATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGa                                                                <  6:247618/148‑1 (MQ=255)
                                                                          aTTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATc                                                              <  6:29782/149‑1 (MQ=255)
                                                                             aCGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCt                                                            <  3:176524/148‑1 (MQ=255)
                                                                              cGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCtgt                                                          <  2:287487/149‑1 (MQ=255)
                                                                                      gTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATc                                                                                                  <  1:395572/101‑1 (MQ=255)
                                                                                      gTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATc                                                                                                  >  2:395572/1‑101 (MQ=255)
                                                                                         cGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCcgac                                               <  6:383044/149‑1 (MQ=255)
                                                                                           gTGGCGAGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCTACGCGTACTTCGCCGAGGTGGCGCGGGGTATCGAAGTCGCCTGCTAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCcgacga                                             <  1:92560/149‑1 (MQ=255)
                                                                                                gcgcTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAAcc                                        >  7:333470/1‑149 (MQ=255)
                                                                                                  gctcgctGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCg                                      <  7:73080/149‑1 (MQ=255)
                                                                                                    tcgctGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCtgt                                                          >  2:88689/1‑127 (MQ=255)
                                                                                                    tcgctGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCtgt                                                          <  1:88689/127‑1 (MQ=255)
                                                                                                    tcgctGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGc                                                        <  5:44132/129‑1 (MQ=255)
                                                                                                    tcgctGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGc                                                        >  6:44132/1‑129 (MQ=255)
                                                                                                      gctGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAg                                   >  6:18237/1‑148 (MQ=255)
                                                                                                      gctGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAg                                   >  7:348662/1‑148 (MQ=255)
                                                                                                               gcgcAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAgcagcg                           >  4:25397/1‑147 (MQ=255)
                                                                                                                 gcagcaCGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGTGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAgcagcgcag                        >  6:80751/1‑148 (MQ=255)
                                                                                                                    gcaCGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTAc                    <  6:396813/149‑1 (MQ=255)
                                                                                                                         gCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTgcg               >  1:89049/1‑149 (MQ=255)
                                                                                                                          ccaccaTCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGaa                                                                                               >  4:312029/1‑68 (MQ=255)
                                                                                                                          ccaccaTCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGaa                                                                                               <  3:312029/68‑1 (MQ=255)
                                                                                                                             ccaTCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGtg           >  6:29745/1‑149 (MQ=255)
                                                                                                                              caTCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGtgc          >  1:178535/1‑149 (MQ=255)
                                                                                                                                        ttGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGaa  >  5:1231/1‑147 (MQ=255)
                                                                                                                                        ttGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGaa  >  5:235329/1‑147 (MQ=255)
                                                                                                                                             |                                                                                                                                             
CCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAA  >  NC_002947/2804244‑2804526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑