Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,822,180 T→A 8.2% D153E (GAT→GAA)  PP_2475 → TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,822,1800TA 8.2% 138.9 / 3.4 49D153E (GAT→GAA) PP_2475TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base A (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GGTGAAGCTGAGCGCAGAGGTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATGGCGCGGT  >  NC_002947/2822042‑2822324
                                                                                                                                          |                                                                                                                                                
ggtgAAGCTGAGCGCAGAGGTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCcaca                                                                                                                                        >  1:387691/1‑149 (MQ=255)
  tgAAGCTGAGCGCAGAGGTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATCGGCGATGCTTTCCACACt                                                                                                                                      >  6:245819/1‑149 (MQ=255)
      gCTGAGCGCAGAGGTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCttt                                                                                                                                             >  8:81499/1‑138 (MQ=255)
      gCTGAGCGCAGAGGTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCttt                                                                                                                                             <  7:81499/138‑1 (MQ=255)
               agagGTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGCATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGCTTCTCTGGCAATGGGCGAAGCTTTCCACACTGCGACGGCCCtt                                                                                                                          >  4:359514/1‑148 (MQ=255)
               agagGTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGAGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGAAGCTTTCCACACTGCGACGGACCtt                                                                                                                          >  4:353649/1‑148 (MQ=255)
                   gTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTAc                                                                                                                       >  2:371731/1‑147 (MQ=255)
                       cTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCGACACTGCGACGGCCGTTTACCAGTtg                                                                                                                 >  4:202525/1‑149 (MQ=255)
                          aTCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTtgt                                                                                                                <  1:14720/147‑1 (MQ=255)
                               tCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGGGACGGCCCTTTACCAGTTGTGGCTCg                                                                                                          <  3:250408/148‑1 (MQ=255)
                                cGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCgg                                                                                                         <  8:246035/148‑1 (MQ=255)
                                 ggAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGt                                                                                                        <  2:387691/148‑1 (MQ=255)
                                    gCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGa                                                                                                    <  5:252611/149‑1 (MQ=255)
                                         tGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTTAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCt                                                                                                                           <  4:292407/121‑1 (MQ=255)
                                         tGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCt                                                                                                                           >  3:292407/1‑121 (MQ=255)
                                            gcCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGa                                                                                                    <  1:151702/141‑1 (MQ=255)
                                            gcCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGa                                                                                                    >  2:151702/1‑141 (MQ=255)
                                              cTCACGCTGCGCGATGGTCCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGCCGGCCCATTACCAGTTGTGGCTCGGTGCGAGTCTGCTGac                                                                                          >  6:395560/1‑148 (MQ=255)
                                               tCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGACGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAg                                                                                          >  2:168569/1‑148 (MQ=255)
                                                 aCGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCaa                                                                                       <  5:161489/149‑1 (MQ=255)
                                                  cGCTGCGCGATGGTGGAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAg                                                                                      <  3:106773/149‑1 (MQ=255)
                                                   gCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGc                                                                                     >  2:309700/1‑149 (MQ=255)
                                                         cgATTGTGCAGATCAAATCATTGCTCGGATATCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACAcc                                                                               <  4:250124/149‑1 (MQ=255)
                                                              gTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTCAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCATTCCACACTGCGACGGCCCTTTACCACTTGTCGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTaa                                                                           >  5:134351/1‑148 (MQ=255)
                                                                        aaTCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGTTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGc                                                                >  6:42656/1‑149 (MQ=255)
                                                                         aTCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCt                                                               <  5:78535/149‑1 (MQ=255)
                                                                                    gATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCa                                                    <  7:122738/149‑1 (MQ=255)
                                                                                            aaTGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATa                                                                         <  3:147165/120‑1 (MQ=255)
                                                                                            aaTGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATa                                                                         >  4:147165/1‑120 (MQ=255)
                                                                                                 aTTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACt                                         >  1:225171/1‑147 (MQ=255)
                                                                                                 aTTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACt                                         >  3:166562/1‑147 (MQ=255)
                                                                                                  ttGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACt                                         >  1:382379/1‑146 (MQ=255)
                                                                                                  ttGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACt                                         >  1:388268/1‑146 (MQ=255)
                                                                                                    gAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGa                                     <  2:382379/148‑1 (MQ=255)
                                                                                                    gAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGa                                     <  2:388268/148‑1 (MQ=255)
                                                                                                        aGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATg                                                  >  6:118744/1‑131 (MQ=255)
                                                                                                        aGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATg                                                  <  5:118744/131‑1 (MQ=255)
                                                                                                        aGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCGCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCAtt                                <  5:245819/149‑1 (MQ=255)
                                                                                                                 gagaCGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACctct                       <  4:166562/149‑1 (MQ=255)
                                                                                                                  agaCGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTg                      <  2:225171/149‑1 (MQ=255)
                                                                                                                    aCGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGac                    <  6:359460/149‑1 (MQ=255)
                                                                                                                      ggTTCTCTGGCAAAGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGACTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACAATTCTGAACTCAGaca                   >  1:270993/1‑148 (MQ=255)
                                                                                                                         tctcTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCTGCCGCTGGAAACAGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACAt               >  3:224460/1‑149 (MQ=255)
                                                                                                                          ctctGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACAt               <  1:371731/148‑1 (MQ=255)
                                                                                                                           gctGGCAATGGGCGATGCTTTGCACACTGCGACGGCCCTTTACCAGTAGTGGCTCGGTGCGAGTCTTCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATc              <  6:134351/147‑1 (MQ=255)
                                                                                                                             tGGCAATGGGCGATGCTTTCCACTCTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATc              <  5:395560/146‑1 (MQ=255)
                                                                                                                               gCAATGGTCGAAGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTg            <  3:353649/146‑1 (MQ=255)
                                                                                                                               gCAATGGGCGAAGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAATCGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTg            <  3:359514/146‑1 (MQ=255)
                                                                                                                                      gCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTCCACCGTAATAGTCGGCCGCTCGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATGGCGCGGt  >  5:217431/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                
GGTGAAGCTGAGCGCAGAGGTAGCTGATCTTTCGGAGCCCATGCGCCTCACGCTGCGCGATGGTGCAGATCAAATCATTGCGCGGATTTCTGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATGGCGCGGT  >  NC_002947/2822042‑2822324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑