Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,852,197 A→C 5.2% intergenic (+108/+97) PP_5531 → / ← PP_2505 hypothetical protein/GAF domain/GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,852,1970AC 5.2% 235.9 / 3.0 77intergenic (+108/+97)PP_5531/PP_2505hypothetical protein/GAF domain/GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (34/38);  new base C (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

GATTACGTGCAGCACATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGC  >  NC_002947/2852051‑2852344
                                                                                                                                                  |                                                                                                                                                   
gATTACGTGCAGCACATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAg                                                                                                                                                   >  5:129944/1‑149 (MQ=255)
  ttACGTGAATCACATGTTCGCCTATCTGGGAGAGTGAATCCTGGACGATGTGGGCGCTAAGCAAGTGTGCCGAGCTCGAGTCTCGGGGGGGTCGCTGTTTGTTTGTCCAGCGATGTGGACTCCATTCCCGCCTCTGGTTGCAATAAggg                                                                                                                                                 <  8:164565/149‑1 (MQ=255)
      gTGCAGCACATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTTATTGCAACCAGGggc                                                                                                                                               >  4:72492/1‑147 (MQ=255)
      gTGCAGCACATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTTATTGCAACCAGGggc                                                                                                                                               <  3:72492/147‑1 (MQ=255)
      gTGCAGCACATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAg                                                                                                                                                   >  4:79603/1‑143 (MQ=255)
      gTGCAGCACATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAg                                                                                                                                                   <  3:79603/143‑1 (MQ=255)
       tGCAGCACATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTACCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGggcggc                                                                                                                                            >  1:314204/1‑149 (MQ=255)
        gcagcaCATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTCGACGCCATTCCCGCCCCTGGATGCAATAAGGggcggc                                                                                                                                            >  8:304836/1‑148 (MQ=255)
         cagcaCATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTTATTGCAACCAGGggcggga                                                                                                                                           <  7:286355/148‑3 (MQ=255)
          agcaCATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGAt                                                                                                                                         <  8:151951/149‑1 (MQ=255)
            cacaTGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGAt                                                                                                                                         >  4:367661/1‑147 (MQ=255)
            cacaTGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGAt                                                                                                                                         >  2:331561/1‑147 (MQ=255)
             acaTGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAg                                                                                                                                      >  8:354734/1‑149 (MQ=255)
              caTGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGt                                                                                                                                     <  8:140146/149‑1 (MQ=255)
               aTGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAg                                                                                                                                                   <  4:338762/134‑1 (MQ=255)
               aTGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAg                                                                                                                                                   >  3:338762/1‑134 (MQ=255)
                 gTTCGCCTACCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTg                                                                                                                                   >  7:8770/1‑148 (MQ=255)
                  ttCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGa                                                                                                                                  <  8:128167/148‑1 (MQ=255)
                    cGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGaaa                                                                                                                                <  5:295807/148‑1 (MQ=255)
                     gcgtATCTGCGAGAGTGAATCCTGCTCGTGGTTGGCGCTAAGCATCTGTGCCGCGCTCTGCTCTGGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCGATTCCGGCCCGTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGc                                                                                                                              <  4:135846/146‑1 (MQ=255)
                     gCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGc                                                                                                                              >  8:77509/1‑149 (MQ=255)
                       cTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGa                                                                                                                            <  6:291650/149‑1 (MQ=255)
                          tCTGCGACAGTGACTCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAg                                                                                                                           <  5:329613/147‑1 (MQ=255)
                              cGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGAt                                                                                                                      <  8:8770/148‑1 (MQ=255)
                               gACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCa                                                                                                                    <  2:166064/149‑1 (MQ=255)
                                      aaTCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCaaa                                                                                                              <  1:189855/148‑1 (MQ=255)
                                      aaTCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGATTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAAt                                                                                                             >  8:369999/1‑149 (MQ=255)
                                       aTCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAAt                                                                                                             <  8:252778/148‑1 (MQ=255)
                                             cACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTaa                                                                                                       <  1:331561/148‑1 (MQ=255)
                                             cACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTaa                                                                                                       <  6:285617/148‑1 (MQ=255)
                                             cACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTa                                                                                                        <  8:280803/147‑1 (MQ=255)
                                                 aGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAg                                                                                                  <  7:77509/149‑1 (MQ=255)
                                                      ggCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGaa                                                                                             >  7:340340/1‑149 (MQ=255)
                                                        cgcTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAAtg                                                                                           >  1:120475/1‑149 (MQ=255)
                                                           tAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAAt                                                                                            <  5:181742/145‑1 (MQ=255)
                                                           tAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAAt                                                                                            >  6:181742/1‑145 (MQ=255)
                                                             aGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGAt                                                                                       <  6:23420/148‑1 (MQ=255)
                                                             aGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATg                                                                                      <  7:354734/149‑1 (MQ=255)
                                                             aGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATg                                                                                      >  1:349165/1‑149 (MQ=255)
                                                                aaCTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCGCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGt                                                                                   <  5:110715/149‑1 (MQ=255)
                                                                     tgCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCt                                                                              >  8:90889/1‑149 (MQ=255)
                                                                       ccGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTcc                                                                            >  3:91027/1‑149 (MQ=255)
                                                                            cTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGAt                                                                       >  1:179537/1‑149 (MQ=255)
                                                                             tCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAACCGCGCTGATCAGGCAAATTGGTAACTCGGCCGATTGTGATGGGTCCCCTCCCGGagc                                                                      >  8:79913/1‑147 (MQ=255)
                                                                                ccACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCg                                                                    >  5:79947/1‑148 (MQ=255)
                                                                                 cACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGga                                                                  <  3:388328/149‑1 (MQ=255)
                                                                                       gggCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCtt                                                            <  5:219799/149‑1 (MQ=255)
                                                                                             tCGCAGTTTGTTTGTCCAGCGATGTGGACGCAATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTc                                                      <  7:125584/149‑1 (MQ=255)
                                                                                               gCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCt                                                    >  6:167461/1‑149 (MQ=255)
                                                                                                   tttgtttgTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAg                                                 >  8:328725/1‑148 (MQ=255)
                                                                                                     tgtttgtCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGa                                               <  7:369999/148‑1 (MQ=255)
                                                                                                       tttgtCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCg                                                                          >  8:339478/1‑119 (MQ=255)
                                                                                                       tttgtCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCg                                                                          <  7:339478/119‑1 (MQ=255)
                                                                                                          gtCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAATGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACg                                          <  3:34195/148‑1 (MQ=255)
                                                                                                           tCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCt                                        >  3:108775/1‑149 (MQ=255)
                                                                                                            ccAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTg                                       >  4:116860/1‑149 (MQ=255)
                                                                                                             cAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTgg                                      >  1:330485/1‑149 (MQ=255)
                                                                                                             cAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTgg                                      >  5:207769/1‑149 (MQ=255)
                                                                                                             cAGCGATGTGCACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAATGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCggg                                      <  1:230710/149‑1 (MQ=255)
                                                                                                                cGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCgg                                                                   >  1:19102/1‑117 (MQ=255)
                                                                                                                cGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCgg                                                                   <  2:19102/117‑1 (MQ=255)
                                                                                                                  aTGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACAt                                 <  3:367661/149‑1 (MQ=255)
                                                                                                                         cGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCCTAACTTCCTCAGGATAACGCTGGCACATAGACAAc                          >  8:325034/1‑149 (MQ=255)
                                                                                                                            cATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTg                       <  2:314204/149‑1 (MQ=255)
                                                                                                                            cATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTg                       <  6:79947/149‑1 (MQ=255)
                                                                                                                              ttCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGt                     <  4:91027/149‑1 (MQ=255)
                                                                                                                                gccGCCCCTTATTGCAACCAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCa                   >  3:175460/2‑149 (MQ=255)
                                                                                                                                cccGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTc                    <  7:304836/148‑1 (MQ=255)
                                                                                                                                     cccTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTcc              <  2:179537/149‑1 (MQ=255)
                                                                                                                                           ttGCAATTAGGGGCGGCGATCAGTTGAAAGCGCGCTGATGAGGCTAATTGGTAAATCCGCCGAATGTGATGCGTACCCTCCCGGATCCGGACATGTTAACTTCCTCAGGAGAACGCTGGCACATAGACAACTTGGTCACGTCCACCATg        >  6:81029/1‑149 (MQ=255)
                                                                                                                                              cAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGttt     <  2:120475/149‑1 (MQ=255)
                                                                                                                                               aaTAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCa                                                                                                                    <  3:327410/37‑1 (MQ=255)
                                                                                                                                               aaTAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCa                                                                                                                    >  4:327410/1‑37 (MQ=255)
                                                                                                                                               aaTAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTg    >  7:217170/1‑149 (MQ=255)
                                                                                                                                               aaTAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTg    >  5:53113/1‑149 (MQ=255)
                                                                                                                                                aTAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTgg   >  8:203868/1‑149 (MQ=255)
                                                                                                                                                 tAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGc  >  3:289523/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATTACGTGCAGCACATGTTCGCCTATCTGCGACAGTGAATCCTGCACGAGGTGGGCGCTAAGCAACTGTGCCGCGCTCGCCACTCGGGGCGGTCGCAGTTTGTTTGTCCAGCGATGTGGACGCCATTCCCGCCCCTGGTTGCAATAAGGGGCGGCGATCAGTTGAAAGCGAGCTGATCAGGCAAATTGGTAACTCAGCCGAATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGC  >  NC_002947/2852051‑2852344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑