Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,978,497 T→A 10.3% intergenic (‑143/+99) PP_2604 ← / ← PP_2605 major facilitator family transporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,978,4970TA10.3% 151.6 / 5.7 55intergenic (‑143/+99)PP_2604/PP_2605major facilitator family transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/22);  new base A (3/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATAGACTGCTCGTTTCTTGAAATTGTATTCAGCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGCCAGATACC  >  NC_002947/2978351‑2978642
                                                                                                                                                  |                                                                                                                                                 
tCATAGACTGCTCGTTTCTTGAAATTGTATTCAGCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTAt                                                                                                                                                 >  7:165778/1‑149 (MQ=255)
     gACTGCTCGTTTCTTGAAATTGTATTCAGCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGATAACCGaa                                                                                                                                             <  5:337666/148‑1 (MQ=255)
        tGCTCGTTTCTTGAAATTGTATTCAGCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGt                                                                                                                                         >  3:224999/1‑149 (MQ=255)
          cTCGTTTCTTGAAATTGTATTCAGCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTg                                                                                                                                       >  1:103763/1‑149 (MQ=255)
          cTCGTTTCTTGAAATTGTATTCAGCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGATAACCGAACAGta                                                                                                                                        <  7:190944/148‑2 (MQ=255)
                  ttGAAATTGTATTCAGCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGcc                                                                                                                               >  8:361213/1‑149 (MQ=255)
                        ttGTATTCAGCTGCGGTGATACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCggcggg                                                                                                                         >  4:147665/1‑149 (MQ=255)
                             ttCAGCTGCGGTGCTAC‑GGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCgg                                                                                                                    <  2:49185/148‑1 (MQ=255)
                                 gCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAgg                                                                                                                >  4:301906/1‑149 (MQ=255)
                                 gCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAgg                                                                                                                >  6:117296/1‑149 (MQ=255)
                                     cGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTa                                                                                                              <  5:117296/147‑1 (MQ=255)
                                       gTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCa                                                                                                          <  5:140948/149‑1 (MQ=255)
                                       gTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCa                                                                                                          >  6:39042/1‑149 (MQ=255)
                                        tGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCAc                                                                                                         <  4:144269/149‑1 (MQ=255)
                                           tACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGt                                                                                                       >  6:128923/1‑148 (MQ=255)
                                                 tGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGaa                                                                                                <  4:224999/149‑1 (MQ=255)
                                                        ccGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAg                                                                                          <  3:301906/148‑1 (MQ=255)
                                                         cGTTTCCCAGGCAGGGGGGCACGCAAGGTCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAg                                                                                          >  6:84255/1‑147 (MQ=255)
                                                               ccAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGATAACCGAACAGtag                                                                                                                                       <  6:162831/96‑3 (MQ=255)
                                                               ccAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGATAACCGAACAGtag                                                                                                                                       >  5:162831/1‑94 (MQ=255)
                                                                caggcaggGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAggg                                                                                             <  6:31032/137‑1 (MQ=255)
                                                                caggcaggGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAggg                                                                                             >  5:31032/1‑137 (MQ=255)
                                                                     aggGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCtt                                                                            >  5:361203/1‑149 (MQ=255)
                                                                      ggggggCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTg                                                                           <  5:39042/149‑1 (MQ=255)
                                                                          ggCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTg                                                                       >  8:320961/1‑149 (MQ=255)
                                                                               gCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGACCGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCGTTGGCCTTGGTTGCCTgc                                                                  >  8:103566/1‑148 (MQ=255)
                                                                                cAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGAt                                                                 <  4:197069/149‑1 (MQ=255)
                                                                                 aaGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATg                                                                <  8:165778/149‑1 (MQ=255)
                                                                                 aaGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATg                                                                <  7:320961/149‑1 (MQ=255)
                                                                                   ggCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATggg                                                              <  6:338561/149‑1 (MQ=255)
                                                                                        gTGGATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTc                                                                                                           <  2:127252/99‑1 (MQ=255)
                                                                                        gTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCaa                                                         >  4:36306/1‑149 (MQ=255)
                                                                                             ttgTGAGCAGTGATGACGGCAGCGTCAATTCATGAGACGGTCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGGGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGt                                                                         <  5:220250/126‑1 (MQ=255)
                                                                                             aggTGAGCAGTGAAGACGGCAGCGTCAATTCAACAGACGGGCTACTGTTCGGTTATCGAACGGTAGCGTTGGCCGGCGGGCGCGGAAGGTATTCACGTCACTGAAGCGTACAACGCGTTGGCCTTGGt                                                                         >  6:220250/2‑128 (MQ=255)
                                                                                                    cAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCaa                                             >  5:212905/1‑149 (MQ=255)
                                                                                                       tGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTAATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGa                                          >  1:270214/1‑149 (MQ=255)
                                                                                                         aggACGGCAGCTTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGa                                          <  4:373882/145‑1 (MQ=255)
                                                                                                         aaGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGAt                                         >  3:373882/1‑148 (MQ=255)
                                                                                                         aaGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATc                                        <  6:361203/149‑1 (MQ=255)
                                                                                                           gACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTg                                      >  3:135929/1‑149 (MQ=255)
                                                                                                            aCGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGc                                     <  5:128923/149‑1 (MQ=255)
                                                                                                             cGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGca                                    <  7:361213/149‑1 (MQ=255)
                                                                                                             cGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGca                                    <  6:212905/149‑1 (MQ=255)
                                                                                                                     tCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCcgc                            >  5:182532/1‑149 (MQ=255)
                                                                                                                        aTTCAAGAGACGGGCTACTGTTCGGTAATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCt                         >  1:10637/1‑149 (MQ=255)
                                                                                                                           cAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCaa                      >  7:189730/1‑149 (MQ=255)
                                                                                                                            aaGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCaa                      <  2:199601/148‑1 (MQ=255)
                                                                                                                                gaCGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGctc                 >  8:18764/1‑149 (MQ=255)
                                                                                                                                         cTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGCCa        <  5:84255/149‑1 (MQ=255)
                                                                                                                                          tGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGc          >  6:36292/1‑146 (MQ=255)
                                                                                                                                            ttCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGCCAGa      >  1:303328/1‑148 (MQ=255)
                                                                                                                                            ttCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGCCAGa      >  3:241971/1‑148 (MQ=255)
                                                                                                                                            ttCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGCCAGa      <  5:26597/148‑1 (MQ=255)
                                                                                                                                              cGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGCCAGATAc   >  7:23689/1‑149 (MQ=255)
                                                                                                                                               ggTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGCCAGATAcc  >  7:259019/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCATAGACTGCTCGTTTCTTGAAATTGTATTCAGCTGCGGTGCTACGGGTGCGTGACCGTTTCCCAGGCAGGGGGGCACGCAAGGCCGGTGCATGGTGAGCAGTGAAGACGGCAGCGTCAATTCAAGAGACGGGCTACTGTTCGGTTATCGAACGGTTGCGTTGGCCGGCGGGCGCGGCAGGTATTCACGTCACTGAAGGGTAGAAGGCTTTGGCCTTGGTTGCCTGATGGGCGCAACACCGACGTCAATGATCTGCACAGCCCGCGCTCAACGCTCTACCGGCCAGATACC  >  NC_002947/2978351‑2978642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑