Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,041,395 G→C 8.2% A199A (GCC→GCG) ‡ PP_2653 ← Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,041,3950GC 8.2% 189.4 / 6.4 61A199A (GCC→GCG) ‡PP_2653Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (31/25);  new base C (3/2);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01

TTCGATGCGCAGTGACTGCACGGTGACATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCTGCAGCAGGTT  >  NC_002947/3041251‑3041541
                                                                                                                                                |                                                                                                                                                  
ttCGATGCGCAGTGACTGCACGGTGACATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCg                                                                                                                                                >  6:161298/1‑149 (MQ=255)
   gATGCGCAGTGACTGCACGGTGACATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGc                                                                                                                                              <  5:324362/148‑1 (MQ=255)
    aTGCGCAGTGACTGCACGGTGACATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGc                                                                                                                                              >  8:159845/1‑147 (MQ=255)
                tGCACGGTGTCATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAgcg                                                                                                                                >  2:65444/1‑149 (MQ=255)
                tGCACGGTGACATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCAACTGGCACTGGCTTGCCGGGGCCGGCAAGTCGCGCAAgcg                                                                                                                                >  7:156965/1‑149 (MQ=255)
                 gCACGGTGACATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAgcgc                                                                                                                               >  4:210062/1‑149 (MQ=255)
                         aattCCTGCTGCATGCTAAAGGTGGTGTTCGTGGTCAGGAAGCGCATCTCACCATACGGGGTGTTCAAGCGGGTCAGCAGCAGAGGTGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCa                                                                                                                        <  8:156965/145‑1 (MQ=255)
                          cATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGt                                                                                                                      <  8:4249/149‑1 (MQ=255)
                            tCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGc                                                                                                                    <  5:121645/149‑1 (MQ=255)
                                       gCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGc                                                                                                                    >  1:118473/1‑138 (MQ=255)
                                       gCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGc                                                                                                                    <  2:118473/138‑1 (MQ=255)
                                        ccAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGc                                                                                                        >  7:117522/1‑149 (MQ=255)
                                         cAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCg                                                                                                       >  3:185689/1‑149 (MQ=255)
                                         cAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCg                                                                                                       <  4:183704/149‑1 (MQ=255)
                                          aaaGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTAACGCGGGTCAGCAGCAGAGGGGTGGCTGTCCTCATCTGGCACGGGCTTGCCCGGGCCGGCAAGACGCGCAAGCGAGCTGCCAGTGCCTGATTGCCTGCgg                                                                                                      >  2:390936/1‑149 (MQ=255)
                                                 gtgAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCt                                                                                               >  5:2727/1‑149 (MQ=255)
                                                   gAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTg                                                                                              >  5:55097/1‑148 (MQ=255)
                                                          gACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGACTTGCCGGCCCCGGCAAgcccg                                                                                                                                       >  8:102804/1‑96 (MQ=255)
                                                          gACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGACTTGCCGGCCCCGGCAAgcccg                                                                                                                                       <  7:102804/100‑5 (MQ=255)
                                                             aGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGAACGTTg                                                                                   >  7:369471/1‑149 (MQ=255)
                                                               gAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGc                                                                                  >  2:64756/1‑148 (MQ=255)
                                                                aaCCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGc                                                                                  >  3:159925/1‑147 (MQ=255)
                                                                aaCCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGc                                                                                  >  3:113785/1‑147 (MQ=255)
                                                                aaCCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGGTGc                                                                                  >  8:121567/1‑147 (MQ=255)
                                                                          cACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGgcagca                                                                         >  2:355881/1‑146 (MQ=255)
                                                                          cACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGgcagca                                                                         <  1:355881/146‑1 (MQ=255)
                                                                          cACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAgcg                                                                      >  5:79551/1‑149 (MQ=255)
                                                                           aCCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGcc                                                                                                                   <  1:163763/103‑1 (MQ=255)
                                                                           aCCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGcc                                                                                                                   >  2:163763/1‑103 (MQ=255)
                                                                           aCCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAgcgc                                                                     <  1:64756/149‑1 (MQ=255)
                                                                            ccATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGcgcg                                                                    <  1:390936/149‑1 (MQ=255)
                                                                              aTACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCATTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGa                                                                  <  1:65444/149‑1 (MQ=255)
                                                                              aTACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGa                                                                  >  4:4627/1‑149 (MQ=255)
                                                                               tACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGAt                                                                 <  3:210062/149‑1 (MQ=255)
                                                                                  ggggTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTc                                                              <  4:159925/149‑1 (MQ=255)
                                                                                    ggTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCg                                                            <  8:117522/149‑1 (MQ=255)
                                                                                    ggTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCg                                                            >  2:145313/1‑149 (MQ=255)
                                                                                      tgtTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGgc                                                          <  7:104137/149‑1 (MQ=255)
                                                                                                ggTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCa                                                <  3:163871/149‑1 (MQ=255)
                                                                                                 gTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAg                                               >  2:318580/1‑149 (MQ=255)
                                                                                                 gTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAg                                               <  6:79551/149‑1 (MQ=255)
                                                                                                 gTCAGCAGCAGAGGGGTGGCCGTCGGCAGCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGGAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCTTTGGCCAg                                               <  3:347419/149‑1 (MQ=255)
                                                                                                     gcagcagAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGa                                           >  4:380994/1‑149 (MQ=255)
                                                                                                        gcagAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCa                                        >  4:382995/1‑149 (MQ=255)
                                                                                                          agagGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCAcc                                      <  5:161298/149‑1 (MQ=255)
                                                                                                               ggTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGac                                 <  7:159845/149‑1 (MQ=255)
                                                                                                                     tGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCgg                           <  4:113785/149‑1 (MQ=255)
                                                                                                                     tGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCgg                           >  8:117737/1‑149 (MQ=255)
                                                                                                                       tcgtcATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCggg                          <  4:185689/148‑1 (MQ=255)
                                                                                                                       tcggcATCTGGCAAGTGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCTAGACCACCggg                          <  8:369471/148‑1 (MQ=255)
                                                                                                                            aTCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCAcag                    >  7:35960/1‑149 (MQ=255)
                                                                                                                             tCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGTTTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCAcag                    >  1:80834/1‑148 (MQ=255)
                                                                                                                                 gCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAgt                <  3:4627/148‑1 (MQ=255)
                                                                                                                                   aCGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTc             >  2:289168/1‑149 (MQ=255)
                                                                                                                                    gcgacTTGCCGGCCCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCt            >  2:340518/5‑149 (MQ=255)
                                                                                                                                    gcgacTTGCCGGCCCCGGCAAGCCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCt            >  8:78724/5‑149 (MQ=255)
                                                                                                                                     cgacTTGCCGGCCCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCTg           <  1:340518/146‑1 (MQ=255)
                                                                                                                                       gCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCTgca         <  7:121567/149‑1 (MQ=255)
                                                                                                                                         ttGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCTgcag        >  4:345720/1‑148 (MQ=255)
                                                                                                                                         ttGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCTgcag        >  6:183547/1‑148 (MQ=255)
                                                                                                                                            ccGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCTGCAGCAgg    >  8:138936/1‑149 (MQ=255)
                                                                                                                                              ggggCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCTGCAGCAGGtt  <  6:2727/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TTCGATGCGCAGTGACTGCACGGTGACATCCTGCGGCATGCCAAAGGTGGTGAACGTGGACAGGAACCGCAGCTCACCATACGGGGTGTTCACGCGGGTCAGCAGCAGAGGGGTGGCTGTCGTCATCTGGCACGGGCTTGCCGGGGCCGGCAAGTCGCGCAAGCGCGCTGCCAGTGCCGGATTGCCTGCGGCCTCCCTGGAGGCACGTTGCCAGGCAGCAGCGCGGATTTCCGGCGCGTTGGCCAGGTGATCACCCAGACCACCGGGGCACAGCAGTGTCTGCAGCAGGTT  >  NC_002947/3041251‑3041541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑