Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,164,097	G→T	9.0%	C296F (TGC→TTC) ‡	PP_2778 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,164,097	0	G	T	9.0%	157.1 / 2.6	52	C296F (TGC→TTC) ‡	PP_2778	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base T (3/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGCGCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGA  >  NC_002947/3163954‑3164240                                                                                                                                                 |                                                                                                                                                 ggcggcGCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGa                                                                                                                                             <  6:280836/149‑1 (MQ=255)    ggcgCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCGTCATgcagg                                                                                                                                          >  4:108646/1‑145 (MQ=255)       gcTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGACAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGAcggcgg                                                                                                                                       <  1:306163/149‑1 (MQ=255)         tGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGcc                                                                                                                                     >  2:138813/1‑149 (MQ=255)          gTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGcc‑c                                                                                                                                   >  1:334443/1‑149 (MQ=255)           ttGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCGTCATGCAggcggca                                                                                                                                     <  3:269720/147‑2 (MQ=255)             ggTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCGTCATGCAggcggcatac                                                                                                                                  <  3:108646/148‑5 (MQ=255)              gggCTGGAAGCCCGCGCGCATGCCGCGCGTCGTCAGCCCTGGTCCCTGGGCCGGGTGCTGGGT‑AACCCCAATCTGTGCGAGGCAGAGAAACTCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGCCC‑CTGGc                                                                                                                               <  8:288894/147‑1 (MQ=255)              gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTgg                                                                                                                                <  7:160962/148‑1 (MQ=255)              gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGc                                                                                                                               <  5:163279/149‑1 (MQ=255)              gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGc                                                                                                                               <  4:182046/149‑1 (MQ=255)               tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                              <  4:98210/149‑1 (MQ=255)               tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                              <  6:25757/149‑1 (MQ=255)               tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                              <  5:234014/149‑1 (MQ=255)                 cTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGa                                                                                                                            >  7:203647/1‑149 (MQ=255)                 cTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGa                                                                                                                            >  6:215228/1‑149 (MQ=255)                    gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                         >  2:274663/1‑149 (MQ=255)                    gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                         >  3:33712/1‑149 (MQ=255)                    gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                         <  4:33712/149‑1 (MQ=255)                         ccGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAAc                                                                                                                    <  1:274663/149‑1 (MQ=255)                                  tGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGTGTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGt                                                                                                            >  1:74954/1‑149 (MQ=255)                                           tcgtcAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgtg                                                                                                   >  1:134095/1‑148 (MQ=255)                                                aGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAt                                                                                             <  2:334443/149‑1 (MQ=255)                                                  gCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                           >  2:194547/1‑149 (MQ=255)                                                  gCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                           >  1:42346/1‑149 (MQ=255)                                                    cTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGa                                                                                         <  1:138813/149‑1 (MQ=255)                                                     tGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGaa                                                                                        >  4:130870/1‑149 (MQ=255)                                                           cTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCa                                                                                  <  2:247206/149‑1 (MQ=255)                                                              ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCAc                                                                                 <  2:134095/147‑1 (MQ=255)                                                              ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACgg                                                                               <  8:203647/149‑1 (MQ=255)                                                              ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACg                                                                                <  5:30620/148‑1 (MQ=255)                                                               gCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGt                                                                              <  1:194547/149‑1 (MQ=255)                                                                           gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgt                                                                                                    <  6:13533/115‑1 (MQ=255)                                                                           gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgt                                                                                                    >  5:13533/1‑115 (MQ=255)                                                                           gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                  >  4:274886/1‑149 (MQ=255)                                                                           gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                  <  2:42346/149‑1 (MQ=255)                                                                            t‑tACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAgg                                                                 <  5:215228/149‑1 (MQ=255)                                                                            ggtACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                  <  2:74954/147‑1 (MQ=255)                                                                                      tCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCCGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGAcc                                                        <  4:285542/149‑1 (MQ=255)                                                                                               ggCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTTGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAgg                                               <  3:274886/149‑1 (MQ=255)                                                                                                     gaAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTgg                                         >  7:45999/1‑149 (MQ=255)                                                                                                      aaaaTCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCGCTGGCCGATGCCGAACTGACCAGTGCTGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTgg                                         >  1:304383/1‑149 (MQ=255)                                                                                                              cTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGgcg                                 >  8:142525/1‑148 (MQ=255)                                                                                                              cTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGgcg                                 >  2:303644/1‑148 (MQ=255)                                                                                                                       ccGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCg                       >  4:310666/1‑149 (MQ=255)                                                                                                                                 agcaAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAACCCCt             >  5:10413/1‑149 (MQ=255)                                                                                                                                    aaGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGa          <  7:142525/149‑1 (MQ=255)                                                                                                                                     ccagggccgccGTCATGCAGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg         >  5:181558/6‑149 (MQ=255)                                                                                                                                      gTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg         >  6:196009/1‑148 (MQ=255)                                                                                                                                      cagggccgccGTCATGCAGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg         >  4:51436/5‑148 (MQ=255)                                                                                                                                       tATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg         >  5:167124/1‑147 (MQ=255)                                                                                                                                            cgccTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGa  >  7:244851/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GGCGGCGCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGA  >  NC_002947/3163954‑3164240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑