Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,164,102	A→C	7.6%	T298P (ACG→CCG) ‡	PP_2778 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,164,102	0	A	C	7.6%	158.2 / 5.1	53	T298P (ACG→CCG) ‡	PP_2778	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base C (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

GGCGGCGCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCAC  >  NC_002947/3163954‑3164247                                                                                                                                                      |                                                                                                                                                   ggcggcGCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGa                                                                                                                                                    <  6:280836/149‑1 (MQ=255)       gcTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGACAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGAcggcgg                                                                                                                                              <  1:306163/149‑1 (MQ=255)         tGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGcc                                                                                                                                            >  2:138813/1‑149 (MQ=255)          gTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGcc‑c                                                                                                                                          >  1:334443/1‑149 (MQ=255)           ttGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCGTCATGCAggcggca                                                                                                                                            <  3:269720/147‑2 (MQ=255)             ggTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCGTCATGCAggcggcatac                                                                                                                                         <  3:108646/148‑5 (MQ=255)              gggCTGGAAGCCCGCGCGCATGCCGCGCGTCGTCAGCCCTGGTCCCTGGGCCGGGTGCTGGGT‑AACCCCAATCTGTGCGAGGCAGAGAAACTCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGCCC‑CTGGc                                                                                                                                      <  8:288894/147‑1 (MQ=255)              gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTgg                                                                                                                                       <  7:160962/148‑1 (MQ=255)              gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGc                                                                                                                                      <  5:163279/149‑1 (MQ=255)              gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGc                                                                                                                                      <  4:182046/149‑1 (MQ=255)               tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                                     <  6:25757/149‑1 (MQ=255)               tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                                     <  5:234014/149‑1 (MQ=255)               tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                                     <  4:98210/149‑1 (MQ=255)                 cTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGa                                                                                                                                   >  6:215228/1‑149 (MQ=255)                 cTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGa                                                                                                                                   >  7:203647/1‑149 (MQ=255)                    gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                                >  3:33712/1‑149 (MQ=255)                    gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                                <  4:33712/149‑1 (MQ=255)                    gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                                >  2:274663/1‑149 (MQ=255)                         ccGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAAc                                                                                                                           <  1:274663/149‑1 (MQ=255)                                  tGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGTGTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGt                                                                                                                   >  1:74954/1‑149 (MQ=255)                                           tcgtcAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgtg                                                                                                          >  1:134095/1‑148 (MQ=255)                                                aGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAt                                                                                                    <  2:334443/149‑1 (MQ=255)                                                  gCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                                  >  1:42346/1‑149 (MQ=255)                                                  gCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                                  >  2:194547/1‑149 (MQ=255)                                                    cTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGa                                                                                                <  1:138813/149‑1 (MQ=255)                                                     tGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGaa                                                                                               >  4:130870/1‑149 (MQ=255)                                                           cTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCa                                                                                         <  2:247206/149‑1 (MQ=255)                                                              ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCAc                                                                                        <  2:134095/147‑1 (MQ=255)                                                              ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACgg                                                                                      <  8:203647/149‑1 (MQ=255)                                                              ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACg                                                                                       <  5:30620/148‑1 (MQ=255)                                                               gCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGt                                                                                     <  1:194547/149‑1 (MQ=255)                                                                           gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgt                                                                                                           <  6:13533/115‑1 (MQ=255)                                                                           gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgt                                                                                                           >  5:13533/1‑115 (MQ=255)                                                                           gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                         <  2:42346/149‑1 (MQ=255)                                                                           gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                         >  4:274886/1‑149 (MQ=255)                                                                            t‑tACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAgg                                                                        <  5:215228/149‑1 (MQ=255)                                                                            ggtACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                         <  2:74954/147‑1 (MQ=255)                                                                                      tCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCCGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGAcc                                                               <  4:285542/149‑1 (MQ=255)                                                                                               ggCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTTGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAgg                                                      <  3:274886/149‑1 (MQ=255)                                                                                                     gaAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTgg                                                >  7:45999/1‑149 (MQ=255)                                                                                                      aaaaTCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCGCTGGCCGATGCCGAACTGACCAGTGCTGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTgg                                                >  1:304383/1‑149 (MQ=255)                                                                                                              cTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGgcg                                        >  2:303644/1‑148 (MQ=255)                                                                                                              cTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGgcg                                        >  8:142525/1‑148 (MQ=255)                                                                                                                       ccGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCg                              >  4:310666/1‑149 (MQ=255)                                                                                                                                 agcaAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAACCCCt                    >  5:10413/1‑149 (MQ=255)                                                                                                                                    aaGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGa                 <  7:142525/149‑1 (MQ=255)                                                                                                                                     ccagggccgccGTCATGCAGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg                >  5:181558/6‑149 (MQ=255)                                                                                                                                      gTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg                >  6:196009/1‑148 (MQ=255)                                                                                                                                      cagggccgccGTCATGCAGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg                >  4:51436/5‑148 (MQ=255)                                                                                                                                       tATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg                >  5:167124/1‑147 (MQ=255)                                                                                                                                            cgccTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGa         >  7:244851/1‑149 (MQ=255)                                                                                                                                                  cATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGa                                                        >  6:382314/1‑96 (MQ=255)                                                                                                                                                  cATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGa                                                        <  5:382314/96‑1 (MQ=255)                                                                                                                                                   aTGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTGACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCAc  >  5:105126/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GGCGGCGCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCAC  >  NC_002947/3163954‑3164247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑