Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,164,103	C→A	7.7%	T298K (ACG→AAG) ‡	PP_2778 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,164,103	0	C	A	7.7%	160.3 / 4.2	52	T298K (ACG→AAG) ‡	PP_2778	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base A (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

GCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCAC  >  NC_002947/3163960‑3164247                                                                                                                                                 |                                                                                                                                                  gcTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGACAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGAcggcgg                                                                                                                                              <  1:306163/149‑1 (MQ=255)   tGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGcc                                                                                                                                            >  2:138813/1‑149 (MQ=255)    gTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGcc‑c                                                                                                                                          >  1:334443/1‑149 (MQ=255)     ttGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCGTCATGCAggcggca                                                                                                                                            <  3:269720/147‑2 (MQ=255)       ggTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCGTCATGCAggcggcatac                                                                                                                                         <  3:108646/148‑5 (MQ=255)        gggCTGGAAGCCCGCGCGCATGCCGCGCGTCGTCAGCCCTGGTCCCTGGGCCGGGTGCTGGGT‑AACCCCAATCTGTGCGAGGCAGAGAAACTCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGCCC‑CTGGc                                                                                                                                      <  8:288894/147‑1 (MQ=255)        gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTgg                                                                                                                                       <  7:160962/148‑1 (MQ=255)        gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGc                                                                                                                                      <  4:182046/149‑1 (MQ=255)        gTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGc                                                                                                                                      <  5:163279/149‑1 (MQ=255)         tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                                     <  6:25757/149‑1 (MQ=255)         tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                                     <  4:98210/149‑1 (MQ=255)         tGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGcc                                                                                                                                     <  5:234014/149‑1 (MQ=255)           cTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGa                                                                                                                                   >  6:215228/1‑149 (MQ=255)           cTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGa                                                                                                                                   >  7:203647/1‑149 (MQ=255)              gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                                >  2:274663/1‑149 (MQ=255)              gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                                >  3:33712/1‑149 (MQ=255)              gAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGc                                                                                                                                <  4:33712/149‑1 (MQ=255)                   ccGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAAc                                                                                                                           <  1:274663/149‑1 (MQ=255)                            tGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGTGTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGt                                                                                                                   >  1:74954/1‑149 (MQ=255)                                     tcgtcAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgtg                                                                                                          >  1:134095/1‑148 (MQ=255)                                          aGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAt                                                                                                    <  2:334443/149‑1 (MQ=255)                                            gCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                                  >  2:194547/1‑149 (MQ=255)                                            gCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                                  >  1:42346/1‑149 (MQ=255)                                              cTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGa                                                                                                <  1:138813/149‑1 (MQ=255)                                               tGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGaa                                                                                               >  4:130870/1‑149 (MQ=255)                                                     cTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCa                                                                                         <  2:247206/149‑1 (MQ=255)                                                        ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCAc                                                                                        <  2:134095/147‑1 (MQ=255)                                                        ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACgg                                                                                      <  8:203647/149‑1 (MQ=255)                                                        ggCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACg                                                                                       <  5:30620/148‑1 (MQ=255)                                                         gCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGt                                                                                     <  1:194547/149‑1 (MQ=255)                                                                     gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgt                                                                                                           >  5:13533/1‑115 (MQ=255)                                                                     gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCtgt                                                                                                           <  6:13533/115‑1 (MQ=255)                                                                     gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                         >  4:274886/1‑149 (MQ=255)                                                                     gT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                         <  2:42346/149‑1 (MQ=255)                                                                      t‑tACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAgg                                                                        <  5:215228/149‑1 (MQ=255)                                                                      ggtACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAg                                                                         <  2:74954/147‑1 (MQ=255)                                                                                tCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCCGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGAcc                                                               <  4:285542/149‑1 (MQ=255)                                                                                         ggCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTTGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAgg                                                      <  3:274886/149‑1 (MQ=255)                                                                                               gaAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTgg                                                >  7:45999/1‑149 (MQ=255)                                                                                                aaaaTCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCGCTGGCCGATGCCGAACTGACCAGTGCTGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTgg                                                >  1:304383/1‑149 (MQ=255)                                                                                                        cTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGgcg                                        >  8:142525/1‑148 (MQ=255)                                                                                                        cTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGgcg                                        >  2:303644/1‑148 (MQ=255)                                                                                                                 ccGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCg                              >  4:310666/1‑149 (MQ=255)                                                                                                                           agcaAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAACCCCt                    >  5:10413/1‑149 (MQ=255)                                                                                                                              aaGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGa                 <  7:142525/149‑1 (MQ=255)                                                                                                                               ccagggccgccGTCATGCAGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg                >  5:181558/6‑149 (MQ=255)                                                                                                                                gTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg                >  6:196009/1‑148 (MQ=255)                                                                                                                                cagggccgccGTCATGCAGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg                >  4:51436/5‑148 (MQ=255)                                                                                                                                 tATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAg                >  5:167124/1‑147 (MQ=255)                                                                                                                                      cgccTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGa         >  7:244851/1‑149 (MQ=255)                                                                                                                                            cATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGa                                                        >  6:382314/1‑96 (MQ=255)                                                                                                                                            cATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGa                                                        <  5:382314/96‑1 (MQ=255)                                                                                                                                             aTGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTGACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCAc  >  5:105126/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GCTGTTGGTGCTGGAATCCCGCGAGCATGCCGAGCGTCGTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGT‑TACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCC‑CTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCAC  >  NC_002947/3163960‑3164247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑