| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,212,276 | T→C | 10.7% | *189W (TGA→TGG) ‡ | PP_2816 ← | transcriptional regulator NfxB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,212,276 | 0 | T | C | 10.7% | 144.2 / 7.9 | 56 | *189W (TGA→TGG) ‡ | PP_2816 | transcriptional regulator NfxB |
| Reads supporting (aligned to +/- strand): ref base T (23/27); new base C (2/4); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01 | |||||||||||
CGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACAT > NC_002947/3212135‑3212424 | cGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAgg < 3:75142/149‑1 (MQ=255)cGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAgg > 2:93627/1‑149 (MQ=255) gCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAggg < 1:110646/149‑1 (MQ=255) gCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCCCTATTGAgcgc < 4:110624/148‑1 (MQ=255) ccGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCCCTATTGAGCGccc < 7:88108/149‑1 (MQ=255) cATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTg < 8:120878/149‑1 (MQ=255) cATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCCCTATTGAGCGCCcgcgca < 7:83779/149‑3 (MQ=255) aTCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTgg < 5:110406/149‑1 (MQ=255) tCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTgg > 4:179017/1‑148 (MQ=255) tCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTgg > 1:56001/1‑148 (MQ=255) tCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTgg > 2:185492/1‑148 (MQ=255) tCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTgg > 7:132600/1‑148 (MQ=255) tCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTACGCGGGCGCCCTATTGAGCGCCcgcgca < 5:252768/147‑3 (MQ=255) tCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTACGCGGGCGCCCTATTGAGCGCCcgcgca > 6:252768/1‑145 (MQ=255) cATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAAt > 6:32751/1‑149 (MQ=255) cATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAAt < 6:238671/149‑1 (MQ=255) cAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGcc < 8:132600/148‑1 (MQ=255) cAGATCGCCGCTGCCCTTGAGCTGGTGATCCGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGccc < 1:367352/149‑1 (MQ=255) gcTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAAc < 1:185492/149‑1 (MQ=255) cTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTa < 6:88843/138‑1 (MQ=255) cTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAAc > 2:348699/1‑143 (MQ=255) cTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAAc < 1:348699/143‑1 (MQ=255) cTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCATGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTa > 5:88843/1‑138 (MQ=255) gAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAAt < 7:335453/127‑1 (MQ=255) gAGCTGGTGATCTGGAGCACGCAGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAAt > 8:335453/1‑127 (MQ=255) aGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATATGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTcc < 1:180231/148‑1 (MQ=255) aGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCa < 2:56001/149‑1 (MQ=255) cTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGTGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAg > 1:240742/1‑148 (MQ=255) tGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCg > 6:44526/1‑149 (MQ=255) aTCTGGAGCACGCAGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGt < 5:93983/148‑1 (MQ=255) gAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGcaca > 8:306211/1‑149 (MQ=255) gccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCTCTCTCCAGAACCGTTGCACActctct > 5:270389/1‑149 (MQ=255) ccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCtt < 3:179017/149‑1 (MQ=255) ctcgcGATGTCGCCGAGCATTACCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTg < 6:270389/145‑1 (MQ=255) cTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGgc < 5:32751/149‑1 (MQ=255) gATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGgcgcg > 2:313196/1‑149 (MQ=255) gATGACGCCGAGCAATAGCGTTCATTCACTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGgcgcg > 4:338123/1‑149 (MQ=255) gACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGgcgcgcgc > 8:10971/1‑149 (MQ=255) ccGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGccccc < 1:313196/149‑1 (MQ=255) aTAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGTGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcg < 2:240742/149‑1 (MQ=255) cGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcgg < 2:285531/146‑1 (MQ=255) cGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcgg > 1:285531/1‑146 (MQ=255) ccttccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatg > 8:160833/1‑149 (MQ=255) tccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgcca > 1:238935/1‑149 (MQ=255) cttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgccat < 7:306211/148‑1 (MQ=255) tttGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgccatg > 6:216008/1‑148 (MQ=255) cGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAg < 6:73412/149‑1 (MQ=255) gCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcgggcg > 7:260648/1‑128 (MQ=255) gCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcgggcg < 8:260648/128‑1 (MQ=255) aaTAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgc < 3:35684/136‑1 (MQ=255) aaTAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgc > 4:35684/1‑136 (MQ=255) cACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACAt < 7:10971/148‑1 (MQ=255) aCGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACAtct < 7:160833/149‑1 (MQ=255) ggcaacagCCGTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCg < 3:143258/142‑1 (MQ=255) tGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAAcgc > 3:172407/1‑149 (MQ=255) acgcgGGCGCCCTATTGAGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAAcgcc > 2:42583/2‑149 (MQ=255) tCAATAGGGCGCCCGCGTGGCAATGCCTTGTAGAAACGCGCTCTCCAGCACCGTTGCCCACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACAt > 1:218633/1‑149 (MQ=255) tCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACAt > 3:222958/1‑149 (MQ=255) | CGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACAT > NC_002947/3212135‑3212424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |