Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,212,977 T→A 7.7% M15K (ATG→AAG)  mexC → multidrug RND transporter membrane fusion protein MexC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,212,9770TA 7.7% 153.9 / 2.9 52M15K (ATG→AAG) mexCmultidrug RND transporter membrane fusion protein MexC
Reads supporting (aligned to +/- strand):  ref base T (25/23);  new base A (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TGAAGGGGTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATGC  >  NC_002947/3212831‑3213117
                                                                                                                                                  |                                                                                                                                            
tGAAGGGGTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGAGGGCGCATTGGCCATgg                                                                                                                                            >  4:372088/1‑149 (MQ=255)
  aaGGGGTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGTCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATg                                                                                                                                             <  6:307342/146‑1 (MQ=255)
      ggTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCg                                                                                                                                      >  3:98803/1‑149 (MQ=255)
       gTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGc                                                                                                                                     >  5:230225/1‑149 (MQ=255)
                 gTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGccaat                                                                                                                           <  1:9859/149‑4 (MQ=255)
                  tCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGgt                                                                                                                          <  6:146320/149‑1 (MQ=255)
                    ttttATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGACCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGgtgt                                                                                                                        >  8:84058/1‑149 (MQ=255)
                            ttttAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCg                                                                                                                 >  3:30563/1‑148 (MQ=255)
                                     gaCAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGCGCCCGCagcagagc                                                                                                        <  4:98803/148‑1 (MQ=255)
                                      aCAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagcag                                                                                                      >  8:148948/1‑149 (MQ=255)
                                        aaTATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGa                                                                                                    >  6:30397/1‑149 (MQ=255)
                                          tatTGACTGATGTGGGTTTGATAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCtt                                                                                                                                  <  6:367433/117‑1 (MQ=255)
                                          tatTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCAGTTTTCAATCAACGTTCGGGTGTGTGATGGGCAAGACAGGATCGATAAGGGTGGGCCCATTGGACATGGCGATCGCCtt                                                                                                                                  >  5:367433/1‑117 (MQ=255)
                                              gACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTg                                                                                               >  1:234787/1‑148 (MQ=255)
                                              gACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTg                                                                                               <  7:148948/148‑1 (MQ=255)
                                              gACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTg                                                                                               <  7:29700/148‑1 (MQ=255)
                                                 tCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGc                                                                                           >  7:206234/1‑149 (MQ=255)
                                                 tCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGc                                                                                           >  8:119501/1‑149 (MQ=255)
                                                   aTGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGcc                                                                                          >  4:37103/1‑148 (MQ=255)
                                                   aTGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGc                                                                                           <  4:30563/147‑1 (MQ=255)
                                                      tgGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAAt                                                                                       <  3:372088/148‑1 (MQ=255)
                                                              aaaaGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTAc                                                                              >  2:206363/1‑149 (MQ=255)
                                                                aaGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTAccc                                                                            >  8:12571/1‑149 (MQ=255)
                                                                aaGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGAGAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTAccc                                                                            >  2:93185/1‑149 (MQ=255)
                                                                 aGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCg                                                                           >  2:195479/1‑149 (MQ=255)
                                                                   tAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGt                                                                         >  1:146433/1‑149 (MQ=255)
                                                                   tAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGt                                                                         <  6:230225/149‑1 (MQ=255)
                                                                     gCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTg                                                                        <  7:84058/148‑1 (MQ=255)
                                                                        tCCGCCCCGTTTTCAATCAAGCTTCGGCGGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGGGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGaaa                                                                    <  2:79109/149‑1 (MQ=255)
                                                                          cGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATc                                                                  <  2:234787/149‑1 (MQ=255)
                                                                          cGCCCAGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATc                                                                  <  3:37103/149‑1 (MQ=255)
                                                                            ccccGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGt                                                                >  5:107949/1‑149 (MQ=255)
                                                                            ccccGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGt                                                                <  2:146433/149‑1 (MQ=255)
                                                                                      aatcaaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGgc                                                       <  5:30397/148‑1 (MQ=255)
                                                                                       atcaaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGa                                                              >  1:230390/1‑140 (MQ=255)
                                                                                       atcaaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGa                                                              <  2:230390/140‑1 (MQ=255)
                                                                                                          aTGCGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTggc                                  >  6:299599/1‑149 (MQ=255)
                                                                                                             ggCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTggcggc                               >  5:260951/1‑149 (MQ=255)
                                                                                                                   aCAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACt                         <  6:260951/149‑1 (MQ=255)
                                                                                                                     aGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACACGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGTCTTGGCGGCTGAACTgc                       >  3:28433/1‑149 (MQ=255)
                                                                                                                        ttCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgccgg                    >  5:318386/1‑149 (MQ=255)
                                                                                                                            aTAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTTGCCTTGGCGGCTGAACTgccgggccg                <  1:206363/149‑1 (MQ=255)
                                                                                                                               aGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGt             <  8:206234/149‑1 (MQ=255)
                                                                                                                                 ggTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTc            >  4:157045/1‑148 (MQ=255)
                                                                                                                                       acacccggCCAAGGCGATCGCCATGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCAGGGTCGCGCCGa     >  3:41166/7‑149 (MQ=255)
                                                                                                                                        gcATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagc                                                                                                        >  5:301133/1‑49 (MQ=255)
                                                                                                                                        gcATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagc                                                                                                        <  6:301133/49‑1 (MQ=255)
                                                                                                                                        gcATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGAt    <  1:195479/149‑1 (MQ=255)
                                                                                                                                          aTTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATgc  <  7:119501/149‑1 (MQ=255)
                                                                                                                                          aTTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATgc  <  6:318386/149‑1 (MQ=255)
                                                                                                                                           ccggCCAAGGCGATCGCCATGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgcc                      >  3:215030/3‑128 (MQ=255)
                                                                                                                                           ccggCCAAGGCGATCGCCATGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgcc                      <  4:215030/126‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TGAAGGGGTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATGC  >  NC_002947/3212831‑3213117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑