Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,266,116	G→C	11.0%	intergenic (+33/+26)	PP_2863 → / ← PP_2864	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,266,116	0	G	C	11.0%	170.8 / 10.2	62	intergenic (+33/+26)	PP_2863/PP_2864	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/26);  new base C (2/5);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GCACAAGACCCGCAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGC  >  NC_002947/3265969‑3266257                                                                                                                                                     |                                                                                                                                               gCACAAGACCCGCAAGTGTTCGTTCCAGGT‑GGTGTGCTGGGGGAATTTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTTTCGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGc                                                                                                                                               <  7:268140/149‑1 (MQ=255)     aaGACCCGCAAGGTTTCGTTCCAGGTGGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCcgc                                                                                                                                            >  1:3832/1‑149 (MQ=255)       gACCCGCAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGCTTTACCCGCGaa                                                                                                                                         <  4:362686/149‑1 (MQ=255)         cccGCAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAg                                                                                                                                       <  7:203272/149‑1 (MQ=255)          ccGCAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGaagag                                                                                                                                      >  1:159409/1‑146 (MQ=38)          ccGCAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAgg                                                                                                                                      >  3:122163/1‑149 (MQ=255)            gCAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGaagaggc                                                                                                                                    <  4:131130/149‑6 (MQ=38)            gCAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCCTTTCGCGGCTTTACCCGCGaagaggc                                                                                                                                    <  6:286357/149‑6 (MQ=255)             cAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGcc                                                                                                                                   <  7:195994/149‑1 (MQ=255)              aaGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCg                                                                                                                                  <  3:175283/149‑1 (MQ=255)                  tttCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGaa                                                                                                                               >  2:370531/1‑148 (MQ=255)                   ttCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGaa                                                                                                                               >  2:183732/1‑147 (MQ=255)                      gTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                                           <  2:159409/148‑3 (MQ=37)                      gTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGt                                                                                                                          <  1:183732/149‑1 (MQ=255)                          cAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAgg                                                                                                                           <  1:225002/144‑1 (MQ=255)                          cAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAgg                                                                                                                           >  2:225002/1‑144 (MQ=255)                          cAGGT‑CGTGTGCTGGGCGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGAt                                                                                                                      <  3:70782/149‑1 (MQ=255)                              t‑ggtgTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATcaca                                                                                                                  <  7:291552/149‑1 (MQ=255)                                            gATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCa                                                                                                      >  1:115462/1‑148 (MQ=255)                                             aTTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑cc                                                                                                   >  7:182194/1‑149 (MQ=255)                                                 tgctgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCTAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGc                                                                                                <  7:7194/148‑1 (MQ=255)                                                   ctgctgcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAAc                                                                                             <  2:3832/149‑1 (MQ=255)                                                        gcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCtt                                                                                        >  4:227292/1‑149 (MQ=255)                                                        gcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCggta                                                                                                                               <  7:352623/111‑3 (MQ=25)                                                        gcACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCggta                                                                                                                               >  8:352623/1‑109 (MQ=25)                                                         cACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCtt                                                                                        <  4:335945/148‑1 (MQ=255)                                                         cACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTg                                                                                       <  1:370531/149‑1 (MQ=255)                                                         cACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTg                                                                                       <  1:15107/149‑1 (MQ=255)                                                           ccAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGGAGATCACATGCCCAAGCCCC‑CCGGCAACCGCGTggg                                                                                     >  2:354994/1‑149 (MQ=255)                                                               gtgtTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGCTGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAAc                                                                                 <  6:76131/149‑1 (MQ=255)                                                                 gtTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAAcc                                                                                >  7:30516/1‑148 (MQ=255)                                                                 gtTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAAcc                                                                                >  1:143624/1‑148 (MQ=255)                                                                             cTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAAc                                                                                 >  7:354153/1‑135 (MQ=255)                                                                              tGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCTGTTGTACCGGCCTCTTCGCGGGTAAAGCCGGGAAAGGGCCGGAAGAGGTCGAAAACATGACCAACCACC‑CCGGCAACCGCTAGGGGCACCGACGCACTACCAGc                                                                  >  4:204589/1‑149 (MQ=255)                                                                                gCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTg                                                                >  2:97418/1‑149 (MQ=255)                                                                                          gACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCg                                                       <  2:115462/148‑1 (MQ=255)                                                                                          gACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGc                                                      >  3:105715/1‑149 (MQ=255)                                                                                            cGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCACTg                                                    <  3:227292/149‑1 (MQ=255)                                                                                               gCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTgg                                                                                      >  5:21960/1‑112 (MQ=255)                                                                                               gCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTgg                                                                                      <  6:21960/112‑1 (MQ=255)                                                                                               gCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCaa                                                 >  3:109336/1‑149 (MQ=255)                                                                                                cGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCaa                                                 >  6:304647/1‑148 (MQ=255)                                                                                                 gTTATCTGCCCCTTGGTTGCTGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAac                                                <  1:97418/149‑1 (MQ=255)                                                                                                     tCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTg                                                                <  8:213014/128‑1 (MQ=255)                                                                                                     tCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTg                                                                >  7:213014/1‑128 (MQ=255)                                                                                                      cTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTca                                          >  7:41106/1‑149 (MQ=255)                                                                                                        gCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCGACCGAGGCACTACCCGGTGCAGCGCCCGCTGCCACACCTcacc                                        >  8:325886/1‑148 (MQ=255)                                                                                                        gCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTcaca                                        >  3:231311/1‑149 (MQ=255)                                                                                                         ccccTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTca                                          <  8:41106/146‑1 (MQ=255)                                                                                                             ttGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGtt                                   >  3:188995/1‑149 (MQ=255)                                                                                                                 ttGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAAACGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTtcat                                >  3:338698/1‑148 (MQ=255)                                                                                                                   gATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTtcatca                              <  4:231311/148‑1 (MQ=255)                                                                                                                       tgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGccc                         >  3:287458/1‑149 (MQ=255)                                                                                                                       tgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCACTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGccc                         >  7:366554/1‑149 (MQ=255)                                                                                                                         cAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGcccct                       <  2:143624/149‑2 (MQ=255)                                                                                                                                   ggCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAggg             >  8:16142/1‑149 (MQ=255)                                                                                                                                    gCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGCCCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGGTCATCACGCCCCATGGGGTAGggc            >  4:293256/1‑149 (MQ=255)                                                                                                                                           cgcgGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGgcggcg        <  4:105715/146‑1 (MQ=255)                                                                                                                                           cgcgGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGcc      <  4:109336/148‑1 (MQ=255)                                                                                                                                              gggTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGc  <  8:182194/149‑1 (MQ=255)                                                                                                                                               ggTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTtca                                 >  6:335924/1‑117 (MQ=255)                                                                                                                                               ggTAAAGCCCCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTtca                                 <  5:335924/117‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               GCACAAGACCCGCAAGGTTTCGTTCCAGGT‑GGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCA‑CCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGC  >  NC_002947/3265969‑3266257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑