Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,393,653 T→A 8.0% T252S (ACA→TCA) ‡ PP_2997 ← protein CnmA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,393,6530TA 8.0% 133.7 / 2.6 50T252S (ACA→TCA) ‡PP_2997protein CnmA
Reads supporting (aligned to +/- strand):  ref base T (25/21);  new base A (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

ACTGGAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGC  >  NC_002947/3393509‑3393799
                                                                                                                                                |                                                                                                                                                  
aCTGGAACAATCCGCGAACGTCCTCCCTCAACCCTCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCt                                                                                                                                                  <  4:5372/147‑1 (MQ=255)
aCTGGAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGc                                                                                                                                                >  6:233085/1‑149 (MQ=255)
    gAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGa                                                                                                                                            >  7:372021/1‑149 (MQ=255)
    gAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGCCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGa                                                                                                                                            <  8:343436/149‑1 (MQ=255)
         aTCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGc                                                                                                                                       <  8:372021/149‑1 (MQ=255)
         aTCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGc                                                                                                                                       <  5:390933/149‑1 (MQ=255)
            cgcgAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCcgc                                                                                                                                    >  1:65588/1‑149 (MQ=255)
             gcgAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCcgcg                                                                                                                                   <  3:232498/149‑1 (MQ=255)
               gAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACATCGACCGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGc                                                                                                                                 <  5:272896/149‑1 (MQ=255)
                  cGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAg                                                                                                                              <  8:60232/149‑1 (MQ=255)
                  cGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAg                                                                                                                              <  5:233085/149‑1 (MQ=255)
                    aCCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTc                                                                                                                                                   <  8:297060/126‑1 (MQ=255)
                    aCCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTc                                                                                                                                                   >  7:297060/1‑126 (MQ=255)
                         cctcAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGcc                                                                                                                         >  5:155666/1‑147 (MQ=255)
                                cATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTc                                                                                                                >  8:88463/1‑149 (MQ=255)
                                cATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTc                                                                                                                >  5:158560/1‑149 (MQ=255)
                                        tCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTcc                                                                                                        >  8:86503/1‑149 (MQ=255)
                                          cATTGTCCTGATGATAGCGGGCGTGCCAATGGTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCTTATCGGCCAGTGTCTGCCCGATATGCCGCGGCTAGGTGCCCAGTa                                                                                                                    >  1:181700/1‑135 (MQ=255)
                                          cATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTa                                                                                                                    >  1:175752/1‑135 (MQ=255)
                                          cATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTa                                                                                                                    <  2:175752/135‑1 (MQ=255)
                                          cATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTccc                                                                                                       >  1:363580/1‑148 (MQ=255)
                                               tCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGGCAGACACTGGCCGatat                                                                                                                                         >  5:142233/1‑109 (MQ=39)
                                               tCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGGCAGACACTGGCCGatat                                                                                                                                         <  6:142233/109‑1 (MQ=39)
                                                   gatgatAGCGGGTGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGGCAGACACTGGCCGATATGc                                                                                                                                       <  8:32232/107‑1 (MQ=38)
                                                   gatgatAGCGGGTGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGGCAGACACTGGCCGATATGc                                                                                                                                       >  7:32232/1‑107 (MQ=38)
                                                    atgatAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTc                                                                                             <  6:155666/148‑1 (MQ=255)
                                                                       tGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTgg                                                                         <  2:65588/149‑1 (MQ=255)
                                                                           gTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGc                                                                     >  1:391681/1‑149 (MQ=255)
                                                                                           cTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATccgcc                                                     <  2:343805/149‑1 (MQ=255)
                                                                                           cTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGAGTCTGCCCGATATGCCGCGGCAAGGTGCCAAGTAGGTCGGTAGTCACGATAAACTTCCCCAGCCCCAGAAACGCAGGCAGCTCGAGCATGATccgcc                                                     >  7:325179/1‑149 (MQ=255)
                                                                                            tCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATccgccg                                                    >  7:70792/1‑149 (MQ=255)
                                                                                              cACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGtt                                                  >  5:164871/1‑149 (MQ=255)
                                                                                               aCTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTc                                                 <  7:86503/149‑1 (MQ=255)
                                                                                                 ttCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGAtt                                                         >  4:253633/1‑138 (MQ=255)
                                                                                                 ttCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGAtt                                                         <  3:253633/139‑2 (MQ=255)
                                                                                                 ttCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGa                                               >  7:85166/1‑149 (MQ=255)
                                                                                                 ttCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGa                                               >  2:268120/1‑149 (MQ=255)
                                                                                                   cGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGAt                                              <  2:363580/148‑1 (MQ=255)
                                                                                                    gAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATcc                                            >  4:154913/1‑149 (MQ=255)
                                                                                                    gAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATcc                                            >  5:122234/1‑149 (MQ=255)
                                                                                                    gAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATc                                             <  7:88463/148‑1 (MQ=255)
                                                                                                          gCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGccc                                      <  8:85166/149‑1 (MQ=255)
                                                                                                                 tGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGGATGATCCGCCGTTCGATCCCGGCCCGCGCCaa                               >  7:162336/1‑149 (MQ=255)
                                                                                                                  ggACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTTTCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAAt                              >  2:383864/1‑149 (MQ=255)
                                                                                                                    aCCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATcc                            >  8:115315/1‑149 (MQ=255)
                                                                                                                        gTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCt                        >  3:259721/1‑149 (MQ=255)
                                                                                                                        ctAGCCCATGCATATCGGCCAGTGTCTGCCCGCTATGCCGCGGCAAGGTGCCCACTAGGTCGGTAGTCCCGCTTATCTTCCCCAGCCCGAGAAACGCTCCCAGCTCGACCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCt                        <  2:358311/148‑1 (MQ=255)
                                                                                                                         tAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGc                         <  8:70792/147‑1 (MQ=255)
                                                                                                                                tGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCcagcag                <  6:122234/149‑1 (MQ=255)
                                                                                                                                         gCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGccc       >  5:110132/1‑149 (MQ=255)
                                                                                                                                             gtgtCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTg   <  1:268120/149‑1 (MQ=255)
                                                                                                                                             gtgtCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGAATGATCCGCAGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGACCGGTg   >  3:269932/1‑149 (MQ=255)
                                                                                                                                              tgtCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGc  <  6:158560/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
ACTGGAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGC  >  NC_002947/3393509‑3393799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑