Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,393,657 C→G 7.7% G250G (GGG→GGC)  PP_2997 ← protein CnmA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,393,6570CG 7.7% 144.7 / 2.3 51G250G (GGG→GGC) PP_2997protein CnmA
Reads supporting (aligned to +/- strand):  ref base C (26/21);  new base G (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

ACTGGAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGCCGAAAC  >  NC_002947/3393509‑3393805
                                                                                                                                                    |                                                                                                                                                    
aCTGGAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGc                                                                                                                                                      >  6:233085/1‑149 (MQ=255)
    gAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGa                                                                                                                                                  >  7:372021/1‑149 (MQ=255)
    gAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGCCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGa                                                                                                                                                  <  8:343436/149‑1 (MQ=255)
         aTCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGc                                                                                                                                             <  8:372021/149‑1 (MQ=255)
         aTCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGc                                                                                                                                             <  5:390933/149‑1 (MQ=255)
            cgcgAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCcgc                                                                                                                                          >  1:65588/1‑149 (MQ=255)
             gcgAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCcgcg                                                                                                                                         <  3:232498/149‑1 (MQ=255)
               gAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACATCGACCGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGc                                                                                                                                       <  5:272896/149‑1 (MQ=255)
                  cGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAg                                                                                                                                    <  8:60232/149‑1 (MQ=255)
                  cGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAg                                                                                                                                    <  5:233085/149‑1 (MQ=255)
                         cctcAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGcc                                                                                                                               >  5:155666/1‑147 (MQ=255)
                                cATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTc                                                                                                                      >  8:88463/1‑149 (MQ=255)
                                cATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTc                                                                                                                      >  5:158560/1‑149 (MQ=255)
                                        tCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTcc                                                                                                              >  8:86503/1‑149 (MQ=255)
                                          cATTGTCCTGATGATAGCGGGCGTGCCAATGGTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCTTATCGGCCAGTGTCTGCCCGATATGCCGCGGCTAGGTGCCCAGTa                                                                                                                          >  1:181700/1‑135 (MQ=255)
                                          cATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTa                                                                                                                          >  1:175752/1‑135 (MQ=255)
                                          cATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTa                                                                                                                          <  2:175752/135‑1 (MQ=255)
                                          cATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTccc                                                                                                             >  1:363580/1‑148 (MQ=255)
                                               tCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGGCAGACACTGGCCGatat                                                                                                                                               <  6:142233/109‑1 (MQ=39)
                                               tCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGGCAGACACTGGCCGatat                                                                                                                                               >  5:142233/1‑109 (MQ=39)
                                                   gatgatAGCGGGTGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGGCAGACACTGGCCGATATGc                                                                                                                                             >  7:32232/1‑107 (MQ=38)
                                                   gatgatAGCGGGTGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGGCAGACACTGGCCGATATGc                                                                                                                                             <  8:32232/107‑1 (MQ=38)
                                                    atgatAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTc                                                                                                   <  6:155666/148‑1 (MQ=255)
                                                                       tGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTgg                                                                               <  2:65588/149‑1 (MQ=255)
                                                                           gTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGc                                                                           >  1:391681/1‑149 (MQ=255)
                                                                                           cTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATccgcc                                                           <  2:343805/149‑1 (MQ=255)
                                                                                           cTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGAGTCTGCCCGATATGCCGCGGCAAGGTGCCAAGTAGGTCGGTAGTCACGATAAACTTCCCCAGCCCCAGAAACGCAGGCAGCTCGAGCATGATccgcc                                                           >  7:325179/1‑149 (MQ=255)
                                                                                            tCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATccgccg                                                          >  7:70792/1‑149 (MQ=255)
                                                                                              cACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGtt                                                        >  5:164871/1‑149 (MQ=255)
                                                                                               aCTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTc                                                       <  7:86503/149‑1 (MQ=255)
                                                                                                 ttCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGAtt                                                               >  4:253633/1‑138 (MQ=255)
                                                                                                 ttCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGAtt                                                               <  3:253633/139‑2 (MQ=255)
                                                                                                 ttCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGa                                                     >  2:268120/1‑149 (MQ=255)
                                                                                                 ttCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGa                                                     >  7:85166/1‑149 (MQ=255)
                                                                                                   cGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGAt                                                    <  2:363580/148‑1 (MQ=255)
                                                                                                    gAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATcc                                                  >  5:122234/1‑149 (MQ=255)
                                                                                                    gAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATcc                                                  >  4:154913/1‑149 (MQ=255)
                                                                                                    gAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATc                                                   <  7:88463/148‑1 (MQ=255)
                                                                                                          gCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGccc                                            <  8:85166/149‑1 (MQ=255)
                                                                                                                 tGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGGATGATCCGCCGTTCGATCCCGGCCCGCGCCaa                                     >  7:162336/1‑149 (MQ=255)
                                                                                                                  ggACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTTTCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAAt                                    >  2:383864/1‑149 (MQ=255)
                                                                                                                    aCCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATcc                                  >  8:115315/1‑149 (MQ=255)
                                                                                                                        gTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCt                              >  3:259721/1‑149 (MQ=255)
                                                                                                                        ctAGCCCATGCATATCGGCCAGTGTCTGCCCGCTATGCCGCGGCAAGGTGCCCACTAGGTCGGTAGTCCCGCTTATCTTCCCCAGCCCGAGAAACGCTCCCAGCTCGACCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCt                              <  2:358311/148‑1 (MQ=255)
                                                                                                                         tAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGc                               <  8:70792/147‑1 (MQ=255)
                                                                                                                                tGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCcagcag                      <  6:122234/149‑1 (MQ=255)
                                                                                                                                         gCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGccc             >  5:110132/1‑149 (MQ=255)
                                                                                                                                             gtgtCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTg         <  1:268120/149‑1 (MQ=255)
                                                                                                                                             gtgtCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGAATGATCCGCAGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGACCGGTg         >  3:269932/1‑149 (MQ=255)
                                                                                                                                              tgtCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGc        <  6:158560/149‑1 (MQ=255)
                                                                                                                                                   gCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCCATCCCGCTTCCAGCAGCTTCTGCCCGGTGCCGaa    >  6:126820/1‑148 (MQ=255)
                                                                                                                                                   gCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGCCGaaa   >  1:67295/1‑149 (MQ=255)
                                                                                                                                                    cccGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGCCGaaa   >  4:237955/1‑148 (MQ=255)
                                                                                                                                                    cccGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGCCGAAAc  <  6:333391/149‑1 (MQ=255)
                                                                                                                                                    cccGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGCCGAAAc  >  8:246693/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACTGGAACAATCCGCGAACGACCTCCCTCAACCATCGATTTCCATTGTCCTGATGATAGCGGGCGTGCCAATGCTGTTTGACCGTGAATCCCTCCACTTCGAACGGGCAGTCATGGACCTGTAGCCCATGCATATCGGCCAGTGTCTGCCCGATATGCCGCGGCAAGGTGCCCAGTAGGTCGGTAGTCCCGATTATCTTCCCCAGCCCGAGAAACGCTGGCAGCTCGAGCATGATCCGCCGTTCGATCCCGGCCCGCGCCAATCCCGCTTCCAGCAGCTTCTGCCCGGTGCCGAAAC  >  NC_002947/3393509‑3393805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑