Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,422,811 T→A 11.4% A323A (GCT→GCA) ‡ PP_3038 → TraC domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,422,8110TA11.4% 157.1 / 11.0 62A323A (GCT→GCA) ‡PP_3038TraC domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (28/26);  new base A (2/5);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

TGCGGTGGTTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATGCG  >  NC_002947/3422667‑3422954
                                                                                                                                                |                                                                                                                                               
tgcGGTGGTTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCg                                                                                                                                             >  3:270811/1‑149 (MQ=255)
tgcGGTGGTTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCg                                                                                                                                             >  2:48845/1‑149 (MQ=255)
    gtggtTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTgg                                                                                                                                         >  5:288529/1‑149 (MQ=255)
     gggtTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGc                                                                                                                                        <  8:316605/148‑1 (MQ=255)
      ggtTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCCCGGCAGTGATCAAGCCACAGGCTCCCGGGGGCt                                                                                                                                       >  8:167814/1‑149 (MQ=255)
      ggtTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCt                                                                                                                                       <  8:306019/149‑1 (MQ=255)
       gtTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGCtt                                                                                                                                      <  4:165789/149‑2 (MQ=255)
          gtgggtggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGCTTGa                                                                                                                                    <  5:136456/148‑1 (MQ=255)
           tgggtggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCACTGATCAAGCCACCGGCTACCGGTGGCTGGaa                                                                                                                                   <  6:343464/148‑1 (MQ=255)
           tgggtggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCAATGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGaa                                                                                                                                   <  6:128645/148‑1 (MQ=255)
             ggtggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGa                                                                                                                                <  7:6552/149‑1 (MQ=255)
              gtggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGAc                                                                                                                               <  4:270811/149‑1 (MQ=255)
                  ccAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGc                                                                                                                           >  7:301513/1‑149 (MQ=255)
                  ccAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGc                                                                                                                           <  3:376511/149‑1 (MQ=255)
                       tAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGcc                                                                                                                      <  5:38277/149‑1 (MQ=255)
                         gTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCa                                                                                                                     <  1:163301/148‑1 (MQ=255)
                         gTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAg                                                                                                                    <  2:195557/149‑1 (MQ=255)
                         gTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAg                                                                                                                    <  2:321874/149‑1 (MQ=255)
                          tCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGCCCTGCATTGCGCTGAAGGCCTTTAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGCAAGGACATGCTCGCCat                                                                                                                    >  7:139066/1‑147 (MQ=255)
                            ggCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGa                                                                                                                  <  2:201401/148‑1 (MQ=255)
                                  aTCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGagag                                                                                                           >  7:36833/1‑149 (MQ=255)
                                     ttctCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGCttg                                                                                                                                     <  3:142534/120‑3 (MQ=255)
                                     ttctCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGCttg                                                                                                                                     >  4:142534/1‑118 (MQ=255)
                                          ggCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGcc                                                                                                    <  7:244039/148‑1 (MQ=255)
                                           gCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGTCCt                                                                                                  >  7:359241/1‑149 (MQ=255)
                                              gAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGAt                                                                                               >  1:216776/1‑149 (MQ=255)
                                                gCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCg                                                                                             >  2:36935/1‑149 (MQ=255)
                                                      ggTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCata                                                                                       <  1:36935/149‑1 (MQ=255)
                                                       gTTAAATGGACCGACTGCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCatat                                                                                      <  8:139066/149‑1 (MQ=255)
                                                             tGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGaa                                                                                <  3:282160/149‑1 (MQ=255)
                                                               gACCGACTTCAACGACCTGCATTGCTCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACg                                                                              >  6:46616/1‑149 (MQ=255)
                                                               gACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGCt                                                                                                                                       >  1:6721/1‑92 (MQ=255)
                                                               gACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGCt                                                                                                                                       <  2:6721/92‑1 (MQ=255)
                                                                         aaCGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGAt                                                                    <  1:48845/149‑1 (MQ=255)
                                                                         aaCGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGAt                                                                    >  6:249675/1‑149 (MQ=255)
                                                                             aCCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTc                                                                            >  7:314057/1‑137 (MQ=255)
                                                                             aCCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTc                                                                            <  8:314057/137‑1 (MQ=255)
                                                                             aCCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTg                                                                >  1:185445/1‑149 (MQ=255)
                                                                               cTGCATTGCGCTGACGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGc                                                              <  7:167814/149‑1 (MQ=255)
                                                                               cTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGc                                                              >  3:22825/1‑149 (MQ=255)
                                                                               cTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGc                                                              <  6:288529/149‑1 (MQ=255)
                                                                                tGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCCACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGc                                                              >  1:98396/1‑148 (MQ=255)
                                                                                   aTTGCGATGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAat                                                          >  6:254302/1‑149 (MQ=255)
                                                                                          tGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCAGATGCAGAACGTCGAACTGATCCTGGCCAATGATGAgc                                                   >  8:16749/1‑149 (MQ=255)
                                                                                           gAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAgcg                                                  >  1:271693/1‑149 (MQ=255)
                                                                                                      ggCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATGGCCCATATGCAGAACTTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCgggg                                       <  2:216776/149‑1 (MQ=255)
                                                                                                          gTTCGCCGGCAAGTCACGGCAGTGTTCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAatg                                                         >  7:241968/1‑127 (MQ=255)
                                                                                                          gTTCGCCGGCAAGTCACGGCAGTGTTCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAatg                                                         <  8:241968/127‑1 (MQ=255)
                                                                                                          gTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGAt                                   >  2:277087/1‑149 (MQ=255)
                                                                                                               ccGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGtt                              <  1:182250/149‑1 (MQ=255)
                                                                                                                       tCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGTCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGcc                      <  8:359241/149‑1 (MQ=255)
                                                                                                                              aGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCt               >  6:337730/1‑149 (MQ=255)
                                                                                                                               gTGATCAAGCCACCGGCTACCGGTGGCTGGAACGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATCAGCGCTGGAGCGCGGTGAACGGTTACAACGCCTTCAGCt               >  7:125044/1‑148 (MQ=255)
                                                                                                                                  aTCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGc                                                              >  2:356575/1‑98 (MQ=255)
                                                                                                                                  aTCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGc                                                              <  1:356575/98‑1 (MQ=255)
                                                                                                                                  aTCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTcc             >  7:65236/1‑147 (MQ=255)
                                                                                                                                    cAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTCATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGTTTACAACGCCTTCAGCTCCAAg          >  3:33361/1‑148 (MQ=255)
                                                                                                                                       gCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCa      >  4:269866/1‑149 (MQ=255)
                                                                                                                                         cACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAgat               >  3:56792/1‑136 (MQ=255)
                                                                                                                                          aCCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATgc   >  7:334379/1‑149 (MQ=255)
                                                                                                                                          aCCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATgc   <  5:249675/149‑1 (MQ=255)
                                                                                                                                           ccGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATgcg  <  8:301513/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TGCGGTGGTTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATGCG  >  NC_002947/3422667‑3422954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑