Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,451,344 C→A 5.1% L701F (TTG→TTT)  PP_3069 ← outer membrane autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,451,3440CA 5.1% 258.6 / 3.1 79L701F (TTG→TTT) PP_3069outer membrane autotransporter
Reads supporting (aligned to +/- strand):  ref base C (33/42);  new base A (2/2);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

CAAAAGGCTGCAACAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCGGGTGTATTGCAA  >  NC_002947/3451199‑3451490
                                                                                                                                                  |                                                                                                                                                   
cAAAAGGCTGCAACAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AAc                                                                                                                                                 >  2:234776/1‑149 (MQ=255)
 aaaaGGCTGCAACAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACt                                                                                                                                                <  1:348563/149‑1 (MQ=255)
 aaaaGGCTGCAACAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACt                                                                                                                                                <  4:206483/149‑1 (MQ=255)
   aaGGCTGCAACAGGCGCCCGCTGCTCCCCGTGAGCGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATA‑AACt                                                                                                                                                >  7:53941/1‑147 (MQ=255)
   aaGGCTGCAACAGGCGCCCGCTGCTCCCCGTGAGCGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATA‑AACt                                                                                                                                                >  7:47727/1‑147 (MQ=255)
      gCTGCAACAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCt                                                                                                                                           >  7:319545/1‑149 (MQ=255)
        tGCAACAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTg                                                                                                                                         >  7:186915/1‑149 (MQ=255)
          cAACAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTg                                                                                                                                         <  1:129430/147‑1 (MQ=255)
           aaCAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAAGACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCg                                                                                                                                       >  1:96970/1‑149 (MQ=255)
           aaCAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCgg                                                                                                                                      >  5:243032/1‑149 (MQ=255)
                   cccGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCAcgc                                                                                                                              <  8:319545/149‑1 (MQ=255)
                    ccGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCAcgcg                                                                                                                             >  4:110534/1‑149 (MQ=255)
                    ccGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCAcgcg                                                                                                                             >  1:270846/1‑149 (MQ=255)
                     cGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACgcgc                                                                                                                            >  7:269581/1‑149 (MQ=255)
                          ctCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAg                                                                                                                         <  1:172767/147‑1 (MQ=255)
                           tCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAgcg                                                                                                                       >  8:120306/1‑148 (MQ=255)
                             ccccTGAACGCACCCCCCAGGCGCACGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTCATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTc                                                                                                                    <  8:145272/149‑1 (MQ=255)
                             cccGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGa                                                                                                                                              >  4:199309/1‑123 (MQ=255)
                             cccGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGa                                                                                                                                              <  3:199309/123‑1 (MQ=255)
                              ccGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCg                                                                                                                   <  5:208127/149‑1 (MQ=255)
                                   aaCGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTGAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATCAAACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACa                                                                                                               <  1:70078/149‑1 (MQ=255)
                                    aCGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAgg                                                                                                             <  4:350324/149‑1 (MQ=255)
                                          ccTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATg                                                                                                       >  5:343479/1‑149 (MQ=255)
                                           cTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATgg                                                                                                      <  2:276462/149‑1 (MQ=255)
                                            tCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATggg                                                                                                     >  8:145873/1‑149 (MQ=255)
                                            tCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATggg                                                                                                     >  4:53646/1‑149 (MQ=255)
                                              cAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCTTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATggg                                                                                                     <  2:113980/147‑1 (MQ=255)
                                              cAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATggg                                                                                                     <  7:238290/147‑1 (MQ=255)
                                              cAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATA‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATggg                                                                                                     <  8:53941/147‑1 (MQ=255)
                                              cAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATA‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATggg                                                                                                     <  8:47727/147‑1 (MQ=255)
                                                      ggCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGAt                                                                                           <  3:121048/149‑1 (MQ=255)
                                                        cccAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTc                                                                                         <  8:105687/149‑1 (MQ=255)
                                                        cccAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGAACTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTc                                                                                         >  1:44370/1‑149 (MQ=255)
                                                          cAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTc                                                                                         >  7:323359/1‑147 (MQ=255)
                                                          cAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCa                                                                                       <  7:145873/149‑1 (MQ=255)
                                                            a‑aCGGCCGGTCAGCTCGCCCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTcccgg                                                                                     >  8:294926/1‑146 (MQ=255)
                                                                ggCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGaa                                                                                  <  3:344507/149‑1 (MQ=255)
                                                                 gCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAg                                                                                 >  6:371343/1‑149 (MQ=255)
                                                                   cGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCt                                                                               >  4:118018/1‑149 (MQ=255)
                                                                     gTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGt                                                                             <  3:53646/149‑1 (MQ=255)
                                                                     gTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGt                                                                             >  3:206707/1‑149 (MQ=255)
                                                                        aGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTcc                                                                           <  3:110534/148‑1 (MQ=255)
                                                                        aGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTc                                                                            >  3:177628/1‑147 (MQ=255)
                                                                         gCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAg                                                                         <  7:230824/149‑1 (MQ=255)
                                                                         gCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCAGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAg                                                                         >  4:375719/1‑149 (MQ=255)
                                                                              aCCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCGCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTg                                                                    <  7:294926/149‑1 (MQ=255)
                                                                               ccTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTgg                                                                   <  8:319304/149‑1 (MQ=255)
                                                                                   ggCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGcc                                                                 <  2:33473/147‑1 (MQ=255)
                                                                                   ggCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGAACTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGcc                                                                 <  2:44370/147‑1 (MQ=255)
                                                                                     cGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCg                                                             <  8:269581/149‑1 (MQ=255)
                                                                                      gTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGa                                                            <  6:343479/149‑1 (MQ=255)
                                                                                        cATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAgg                                                          >  4:280287/1‑149 (MQ=255)
                                                                                         aTGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGt                                                         >  5:187354/1‑149 (MQ=255)
                                                                                          tGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCCGCGCGCCCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCCCGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGtt                                                        >  5:232825/1‑149 (MQ=255)
                                                                                                aTGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCgt                                                  <  2:270846/149‑1 (MQ=255)
                                                                                                   cgcgAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTc                                               >  2:261180/1‑149 (MQ=255)
                                                                                                   cgcgAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCAGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTc                                               <  3:375719/149‑1 (MQ=255)
                                                                                                    gcgAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCg                                              >  7:111733/1‑149 (MQ=255)
                                                                                                      gagcCGCTGGCGCGGGCGCTTTCCAGCGCGACCGTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGcc                                            <  8:111733/149‑1 (MQ=255)
                                                                                                          cGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGc                                        <  3:118018/149‑1 (MQ=255)
                                                                                                            cTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTc                                      >  2:237151/1‑149 (MQ=255)
                                                                                                             tGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGt                                                         <  4:178798/129‑1 (MQ=255)
                                                                                                             tGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGt                                                         >  3:178798/1‑129 (MQ=255)
                                                                                                             tGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCg                                     >  3:236344/1‑149 (MQ=255)
                                                                                                                 gcgGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGc                                 <  1:157551/149‑1 (MQ=255)
                                                                                                                 gcgGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGc                                 <  1:162611/149‑1 (MQ=255)
                                                                                                                 gcgGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACACGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGc                                 >  6:196256/1‑149 (MQ=255)
                                                                                                                     gcgcTTTCCAGAGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGgc                             <  8:186915/149‑1 (MQ=255)
                                                                                                                         tttCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCgg                         >  5:397171/1‑149 (MQ=255)
                                                                                                                               gcgcGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATcc                   <  2:96970/149‑1 (MQ=255)
                                                                                                                                     ccTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCgg             <  6:243032/149‑1 (MQ=255)
                                                                                                                                     ccTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCACGCGTGGCCGTCGAGGTTCAGCGTGGCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCgg             <  4:206707/149‑1 (MQ=255)
                                                                                                                                       ttttGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGcgcg                          <  6:238446/134‑1 (MQ=255)
                                                                                                                                       ttttGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGcgcg                          >  5:238446/1‑134 (MQ=255)
                                                                                                                                       ttttGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCGGgt           >  4:388374/1‑149 (MQ=255)
                                                                                                                                         ttGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCGGgtg          <  7:120306/148‑1 (MQ=255)
                                                                                                                                         ttGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCGGgt           <  1:234776/147‑1 (MQ=255)
                                                                                                                                           gcgcAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCGGGTGTa        <  3:280287/148‑1 (MQ=255)
                                                                                                                                                aTC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCGGGTGTATTGCaa  <  6:397171/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CAAAAGGCTGCAACAGGCGCCCGCTGCTCCCCGTGAACGCACCCTCCAGGCGCAGGCCCAA‑ACGGCCGGTCAGCTCGACCTGGGCGTCATGACTGATGCGCGAGACGCTGTCGCGGGCGCTTTCCAGCGCGACCTTTTGCGCAATC‑AACTGAGCTTGCGGTTCCACGCGCCAGCGCTCGGACAGGGTGATGGGGTAGCCCGATTCCAGTGAAGCTGTCCAGGCGTGGCCGTCGAGGTTCAGCGTGTCGCCACGCTCGGAGCGGGCGCGGCCATCCAGGCGGGTGTATTGCAA  >  NC_002947/3451199‑3451490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑