Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 34,269 G→C 5.2% D236H (GAT→CAT)  czrSA → metal‑sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294734,2690GC 5.2% 245.6 / 2.5 76D236H (GAT→CAT) czrSAmetal‑sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (40/32);  new base C (2/2);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

CGCAGAGGCCTGCTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCGGGAGGTTCTGGAGTCGTGCA  >  NC_002947/34122‑34416
                                                                                                                                                   |                                                                                                                                                   
cgcAGAGGCCTGCTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGga                                                                                                                                                    <  5:82798/149‑1 (MQ=255)
 gcAGAGGCCTGCTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGgat                                                                                                                                                   >  5:209144/1‑149 (MQ=255)
  cAGAGGCCTGCTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGgat                                                                                                                                                   >  2:183316/1‑148 (MQ=255)
  cAGAGGCCTGCTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTACCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGgat                                                                                                                                                   >  2:135966/1‑148 (MQ=255)
       gCCTGCTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATggg                                                                                                                                             <  1:183316/149‑1 (MQ=255)
       gCCTGCTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATggg                                                                                                                                             <  8:262346/149‑1 (MQ=255)
            cTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATCCAGGCGAGCCAGCatgatgttgat                                                                                                                                          <  5:35774/147‑6 (MQ=255)
             tACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTcag                                                                                                                                       >  1:29238/1‑149 (MQ=255)
                cACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTcagcag                                                                                                                                    >  6:108076/1‑149 (MQ=255)
                cACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATCCAGGCGAGCCAGCatgatgttgat                                                                                                                                          >  8:284703/1‑138 (MQ=255)
                cACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATCCAGGCGAGCCAGCatgatgttgat                                                                                                                                          <  7:284703/143‑6 (MQ=255)
                     aCCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGctc                                                                                                                                 <  6:209144/147‑1 (MQ=255)
                     aCCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCtctc                                                                                                                               >  3:98540/1‑149 (MQ=255)
                     aCCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCtctc                                                                                                                               >  3:80037/1‑149 (MQ=255)
                      ccAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCg                                                                                                                              <  4:80037/149‑1 (MQ=255)
                       cAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCg                                                                                                                              >  8:52388/1‑148 (MQ=255)
                        aaGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGCCCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCa                                                                                                                            >  5:378587/1‑149 (MQ=255)
                              cTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGtttt                                                                                                                       <  2:29238/148‑1 (MQ=255)
                               tCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCt                                                                                                                     >  1:125251/1‑149 (MQ=255)
                                 aaGATCGCCTCGAAAATCTCCACCGCGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGCACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTgc                                                                                                                   >  5:72736/1‑148 (MQ=255)
                                   gATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTgac                                                                                                                  >  5:189657/1‑148 (MQ=255)
                                          tCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCa                                                                                                                            <  4:177061/131‑1 (MQ=255)
                                          tCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCa                                                                                                                            >  3:177061/1‑131 (MQ=255)
                                          tCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGc                                                                                                          >  6:131550/1‑149 (MQ=255)
                                                    cAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACTGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACCACCTTGCTCACGAACtt                                                                                                >  7:267088/1‑149 (MQ=255)
                                                     aaCCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGGAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTc                                                                                               >  1:79992/1‑149 (MQ=255)
                                                     aaCCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCATAAATCAATATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGTACTTc                                                                                               <  7:143836/149‑1 (MQ=255)
                                                     aaCCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTc                                                                                               <  3:135351/149‑1 (MQ=255)
                                                     aaCCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTc                                                                                               >  4:135351/1‑149 (MQ=255)
                                                       ccGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGa                                                                                             <  1:369491/149‑1 (MQ=255)
                                                        cGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAg                                                                                            <  6:189657/149‑1 (MQ=255)
                                                        cGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAg                                                                                            >  2:130778/1‑149 (MQ=255)
                                                             gCCTCGACCATCGCCTTCCAACTGATGGGATGCTGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCAcc                                                                                        <  1:346182/148‑1 (MQ=255)
                                                                  gACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTc                                                                                  <  2:125251/149‑1 (MQ=255)
                                                                     cATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGAAGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCaa                                                                               >  6:220824/1‑149 (MQ=255)
                                                                      aTCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAAc                                                                              <  1:307494/149‑1 (MQ=255)
                                                                       tCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAAcc                                                                             >  1:334563/1‑149 (MQ=255)
                                                                        cGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCt                                                                            >  6:89248/1‑149 (MQ=255)
                                                                          ccTTCCAACTGATGGGATGCCTGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGTCTCGCCTTGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTTCAACCTGa                                                                          <  7:153004/149‑1 (MQ=255)
                                                                            ttCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATg                                                                        >  5:342286/1‑149 (MQ=255)
                                                                              ccAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTAACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTgggcc                                                                       <  8:245821/148‑5 (MQ=255)
                                                                               cAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATggg                                                                      >  8:39443/1‑148 (MQ=255)
                                                                                aaCTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGc                                                                     >  2:31211/1‑148 (MQ=255)
                                                                                aaCTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGc                                                                     >  1:267528/1‑148 (MQ=255)
                                                                                aaCTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACAAACTTCGAGCACCGCTTTCCAACCTGATGGGc                                                                     >  4:383536/1‑148 (MQ=255)
                                                                                 aCTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCaa                                                                   <  3:121626/149‑1 (MQ=255)
                                                                                   tGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAgg                                                                 >  5:157104/1‑149 (MQ=255)
                                                                                     aTGGGATGCCTGCAGATCTAAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACGTTGCTCTCGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAgg                                                                 <  2:375838/147‑1 (MQ=255)
                                                                                       gggATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGcc                                                               >  2:257407/1‑147 (MQ=255)
                                                                                        ggATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAg                                                            <  4:98540/149‑1 (MQ=255)
                                                                                        ggATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACAAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCa                                                             <  3:383536/148‑1 (MQ=255)
                                                                                         gATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGTCAAGGcaacct                                                           >  8:238024/1‑144 (MQ=255)
                                                                                         gATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAgg                                                           >  1:211757/1‑149 (MQ=255)
                                                                                          aTGCCGGCTGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCACTCGCAGATTTCTGACGACCTTGCTCACGAACTTCGAGCTCCGGTTTCGAACATGATGGGCAAGGCCAAGGt                                                          >  7:304399/1‑149 (MQ=255)
                                                                                          aTGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGt                                                          >  4:267090/1‑149 (MQ=255)
                                                                                               ggCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGCCCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCt                                                     >  5:280093/1‑149 (MQ=255)
                                                                                               ggCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCt                                                     >  4:194812/1‑149 (MQ=255)
                                                                                                   gAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGAc                                                 <  2:79992/149‑1 (MQ=255)
                                                                                                   gAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGAc                                                 >  6:293056/1‑149 (MQ=255)
                                                                                                    aaCTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGCCCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGAc                                                 >  7:174870/1‑148 (MQ=255)
                                                                                                      cTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGAc                                                 >  7:240020/1‑146 (MQ=255)
                                                                                                         aaaaTGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCagag                                            >  4:83455/1‑148 (MQ=255)
                                                                                                         aaaaTGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCaga                                             <  3:194812/147‑1 (MQ=255)
                                                                                                               atCGCCCACGGCAGCAACATGATGCTTGCTCGGCTGGATGATGGGGTGCAGCAGCTCTCGCAGTTTTGTGACGACCTTGATCTCGAACTTCGAGCACCGCTTTCCACCCTGATGGGCAAGGCGCAGGTTGCCTTGACGAGAgatcgatc                                     <  8:304399/148‑1 (MQ=255)
                                                                                                                  gCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCg                                    <  1:130778/147‑1 (MQ=255)
                                                                                                                  gCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGc                                   <  2:211757/148‑1 (MQ=255)
                                                                                                                   ccGACGGCATCAACATCATGCTGGCTCGCGTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCtt                                 <  5:293056/149‑1 (MQ=255)
                                                                                                                   ccGAAGGCATCTACTTCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTGGCAGTTTTCTGACGACCTTTCTCACTAACTTCGAGCACCGCTTTCGAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCtt                                 <  5:400222/149‑1 (MQ=255)
                                                                                                                      aCGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCACCCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTcc                              <  6:72736/149‑1 (MQ=255)
                                                                                                                        ggCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGa                            <  7:52388/149‑1 (MQ=255)
                                                                                                                        ggCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGa                            <  6:378587/149‑1 (MQ=255)
                                                                                                                             ccccatcatCCAGGCGAGCCAGCATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTAcc                       >  1:23521/4‑149 (MQ=255)
                                                                                                                              aaCATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCg                      >  3:194600/1‑149 (MQ=255)
                                                                                                                               aCATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCgg                     >  1:20587/1‑149 (MQ=255)
                                                                                                                                 atcatGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATTGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCgg                     >  4:274351/1‑147 (MQ=255)
                                                                                                                                  tcatGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCGGGAg                  <  5:131550/149‑1 (MQ=255)
                                                                                                                                     tGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCGGGAGGtt               >  2:127823/1‑149 (MQ=255)
                                                                                                                                            tCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCGGGAGGTTCTGGAGt        >  2:41814/1‑149 (MQ=255)
                                                                                                                                               ccTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCGGGAGGTTCTGGAGTCGt     <  3:274351/149‑1 (MQ=255)
                                                                                                                                                cTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCGGGAGGTTCTGGAGTCGTg    <  6:157104/149‑1 (MQ=255)
                                                                                                                                                   gatgatGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCGGGAGGTTCTGGAGTCGTGca  >  1:317827/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCAGAGGCCTGCTACCACTGACCAAGTTCCTCAAGATCGCCTCGAAAATCTCAACCGAGAGCCTCGACCATCGCCTTCCAACTGATGGGATGCCGGCAGAACTCAAAATGCTCGCCGACGGCATCAACATCATGCTGGCTCGCCTGGATGATGGGGTTCAGCAGCTCTCGCAGTTTTCTGACGACCTTGCTCACGAACTTCGAGCACCGCTTTCCAACCTGATGGGCAAGGCCCAGGTTGCCTTGACCAGAGAACGATCGCTTTCCGAGTACCGGGAGGTTCTGGAGTCGTGCA  >  NC_002947/34122‑34416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑